Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS14545 and ABZR86_RS14550 are separated by 119 nucleotides ABZR86_RS14550 and ABZR86_RS14555 are separated by 153 nucleotides ABZR86_RS14555 and ABZR86_RS14560 are separated by 172 nucleotides
ABZR86_RS14545: ABZR86_RS14545 - pyridoxal-phosphate dependent enzyme, at 3,257,551 to 3,258,501
_RS14545
ABZR86_RS14550: ABZR86_RS14550 - Lrp/AsnC family transcriptional regulator, at 3,258,621 to 3,259,091
_RS14550
ABZR86_RS14555: ABZR86_RS14555 - pirin family protein, at 3,259,245 to 3,259,946
_RS14555
ABZR86_RS14560: ABZR86_RS14560 - LysR substrate-binding domain-containing protein, at 3,260,119 to 3,261,036
_RS14560
Position (kb)
3259
3260 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3258.433 kb on - strand at 3258.539 kb on + strand at 3258.539 kb on + strand at 3258.539 kb on + strand at 3258.539 kb on + strand at 3258.540 kb on - strand at 3258.540 kb on - strand at 3258.540 kb on - strand at 3258.921 kb on + strand, within ABZR86_RS14550 at 3258.922 kb on - strand, within ABZR86_RS14550 at 3258.942 kb on + strand, within ABZR86_RS14550 at 3258.942 kb on + strand, within ABZR86_RS14550 at 3258.978 kb on + strand, within ABZR86_RS14550 at 3259.089 kb on + strand at 3259.090 kb on - strand at 3259.090 kb on - strand at 3259.143 kb on + strand at 3259.143 kb on + strand at 3259.143 kb on + strand at 3259.143 kb on + strand at 3259.143 kb on + strand at 3259.144 kb on - strand at 3259.144 kb on - strand at 3259.144 kb on - strand at 3259.152 kb on + strand at 3259.152 kb on + strand at 3259.152 kb on + strand at 3259.153 kb on - strand at 3259.153 kb on - strand at 3259.166 kb on + strand at 3259.246 kb on + strand at 3259.388 kb on - strand, within ABZR86_RS14555 at 3259.450 kb on + strand, within ABZR86_RS14555 at 3259.450 kb on + strand, within ABZR86_RS14555 at 3259.451 kb on - strand, within ABZR86_RS14555 at 3259.451 kb on - strand, within ABZR86_RS14555 at 3259.456 kb on + strand, within ABZR86_RS14555 at 3259.457 kb on - strand, within ABZR86_RS14555 at 3259.558 kb on + strand, within ABZR86_RS14555 at 3259.687 kb on + strand, within ABZR86_RS14555 at 3259.688 kb on - strand, within ABZR86_RS14555 at 3259.747 kb on + strand, within ABZR86_RS14555 at 3259.747 kb on + strand, within ABZR86_RS14555 at 3259.747 kb on + strand, within ABZR86_RS14555 at 3259.747 kb on + strand, within ABZR86_RS14555 at 3259.748 kb on - strand, within ABZR86_RS14555 at 3259.748 kb on - strand, within ABZR86_RS14555 at 3259.748 kb on - strand, within ABZR86_RS14555 at 3259.748 kb on - strand, within ABZR86_RS14555 at 3259.844 kb on + strand, within ABZR86_RS14555 at 3259.845 kb on - strand, within ABZR86_RS14555 at 3260.085 kb on + strand at 3260.085 kb on + strand at 3260.224 kb on + strand, within ABZR86_RS14560 at 3260.225 kb on - strand, within ABZR86_RS14560 at 3260.593 kb on + strand, within ABZR86_RS14560 at 3260.594 kb on - strand, within ABZR86_RS14560 at 3260.699 kb on - strand, within ABZR86_RS14560 at 3260.851 kb on + strand, within ABZR86_RS14560 at 3260.851 kb on + strand, within ABZR86_RS14560 at 3260.852 kb on - strand, within ABZR86_RS14560 at 3260.926 kb on + strand, within ABZR86_RS14560 at 3260.926 kb on + strand, within ABZR86_RS14560 at 3260.926 kb on + strand, within ABZR86_RS14560 at 3260.927 kb on - strand, within ABZR86_RS14560 at 3260.927 kb on - strand, within ABZR86_RS14560 at 3260.927 kb on - strand, within ABZR86_RS14560 at 3260.927 kb on - strand, within ABZR86_RS14560
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 3,258,433 - +1.0 3,258,539 + -0.1 3,258,539 + +0.1 3,258,539 + -1.2 3,258,539 + +0.0 3,258,540 - +0.6 3,258,540 - -0.2 3,258,540 - +0.9 3,258,921 + ABZR86_RS14550 0.64 -0.1 3,258,922 - ABZR86_RS14550 0.64 -0.2 3,258,942 + ABZR86_RS14550 0.68 -0.0 3,258,942 + ABZR86_RS14550 0.68 +0.3 3,258,978 + ABZR86_RS14550 0.76 +0.4 3,259,089 + -0.2 3,259,090 - -0.2 3,259,090 - +0.9 3,259,143 + -0.9 3,259,143 + +0.3 3,259,143 + +1.4 3,259,143 + -2.3 3,259,143 + -0.1 3,259,144 - -0.4 3,259,144 - -0.9 3,259,144 - -0.2 3,259,152 + +1.8 3,259,152 + +0.8 3,259,152 + -0.9 3,259,153 - -0.6 3,259,153 - -0.1 3,259,166 + +0.5 3,259,246 + +1.0 3,259,388 - ABZR86_RS14555 0.20 -0.8 3,259,450 + ABZR86_RS14555 0.29 -0.2 3,259,450 + ABZR86_RS14555 0.29 -1.0 3,259,451 - ABZR86_RS14555 0.29 -0.1 3,259,451 - ABZR86_RS14555 0.29 -1.3 3,259,456 + ABZR86_RS14555 0.30 +0.2 3,259,457 - ABZR86_RS14555 0.30 -0.1 3,259,558 + ABZR86_RS14555 0.45 -0.4 3,259,687 + ABZR86_RS14555 0.63 +0.5 3,259,688 - ABZR86_RS14555 0.63 +1.2 3,259,747 + ABZR86_RS14555 0.72 -0.7 3,259,747 + ABZR86_RS14555 0.72 +0.4 3,259,747 + ABZR86_RS14555 0.72 +0.8 3,259,747 + ABZR86_RS14555 0.72 -0.8 3,259,748 - ABZR86_RS14555 0.72 -0.4 3,259,748 - ABZR86_RS14555 0.72 -0.4 3,259,748 - ABZR86_RS14555 0.72 -0.6 3,259,748 - ABZR86_RS14555 0.72 -0.0 3,259,844 + ABZR86_RS14555 0.85 -2.4 3,259,845 - ABZR86_RS14555 0.85 -0.4 3,260,085 + +0.4 3,260,085 + -0.5 3,260,224 + ABZR86_RS14560 0.11 -0.3 3,260,225 - ABZR86_RS14560 0.12 -1.0 3,260,593 + ABZR86_RS14560 0.52 -0.8 3,260,594 - ABZR86_RS14560 0.52 -0.7 3,260,699 - ABZR86_RS14560 0.63 +0.2 3,260,851 + ABZR86_RS14560 0.80 -0.3 3,260,851 + ABZR86_RS14560 0.80 +0.1 3,260,852 - ABZR86_RS14560 0.80 -0.2 3,260,926 + ABZR86_RS14560 0.88 -0.2 3,260,926 + ABZR86_RS14560 0.88 -0.7 3,260,926 + ABZR86_RS14560 0.88 -0.1 3,260,927 - ABZR86_RS14560 0.88 -0.9 3,260,927 - ABZR86_RS14560 0.88 -0.5 3,260,927 - ABZR86_RS14560 0.88 +0.2 3,260,927 - ABZR86_RS14560 0.88 +0.1
Or see this region's nucleotide sequence