Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS14250 and ABZR86_RS14255 are separated by 5 nucleotides ABZR86_RS14255 and ABZR86_RS14260 are separated by 3 nucleotides ABZR86_RS14260 and ABZR86_RS14265 overlap by 4 nucleotides
ABZR86_RS14250: ABZR86_RS14250 - PhzF family phenazine biosynthesis isomerase, at 3,172,022 to 3,172,870
_RS14250
ABZR86_RS14255: ABZR86_RS14255 - pyridoxal 5'-phosphate synthase, at 3,172,876 to 3,173,505
_RS14255
ABZR86_RS14260: ABZR86_RS14260 - hypothetical protein, at 3,173,509 to 3,173,874
_RS14260
ABZR86_RS14265: ABZR86_RS14265 - anthranilate synthase family protein, at 3,173,871 to 3,175,730
_RS14265
Position (kb)
3173
3174 Strain fitness (log2 ratio)
-2
-1
0
1 at 3172.863 kb on + strand at 3172.863 kb on + strand at 3172.864 kb on - strand at 3172.864 kb on - strand at 3172.864 kb on - strand at 3172.983 kb on - strand, within ABZR86_RS14255 at 3173.216 kb on + strand, within ABZR86_RS14255 at 3173.216 kb on + strand, within ABZR86_RS14255 at 3173.495 kb on + strand at 3173.808 kb on - strand, within ABZR86_RS14260 at 3173.808 kb on - strand, within ABZR86_RS14260 at 3173.808 kb on - strand, within ABZR86_RS14260 at 3173.859 kb on + strand at 3173.865 kb on + strand at 3173.866 kb on - strand at 3174.079 kb on + strand, within ABZR86_RS14265 at 3174.080 kb on - strand, within ABZR86_RS14265
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 3,172,863 + -0.6 3,172,863 + +0.1 3,172,864 - -0.2 3,172,864 - -0.3 3,172,864 - +0.1 3,172,983 - ABZR86_RS14255 0.17 +0.3 3,173,216 + ABZR86_RS14255 0.54 -0.0 3,173,216 + ABZR86_RS14255 0.54 +1.2 3,173,495 + -0.0 3,173,808 - ABZR86_RS14260 0.82 -0.5 3,173,808 - ABZR86_RS14260 0.82 -1.1 3,173,808 - ABZR86_RS14260 0.82 -1.7 3,173,859 + +0.2 3,173,865 + +0.2 3,173,866 - -0.1 3,174,079 + ABZR86_RS14265 0.11 +0.8 3,174,080 - ABZR86_RS14265 0.11 -2.2
Or see this region's nucleotide sequence