Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS13360 and ABZR86_RS13365 are separated by 94 nucleotides ABZR86_RS13365 and ABZR86_RS13370 overlap by 4 nucleotides ABZR86_RS13370 and ABZR86_RS13375 overlap by 14 nucleotides ABZR86_RS13375 and ABZR86_RS13380 are separated by 21 nucleotides ABZR86_RS13380 and ABZR86_RS13385 are separated by 66 nucleotides
ABZR86_RS13360: ABZR86_RS13360 - TonB-dependent receptor, at 2,997,173 to 2,999,269
_RS13360
ABZR86_RS13365: ABZR86_RS13365 - cupin domain-containing protein, at 2,999,364 to 2,999,816
_RS13365
ABZR86_RS13370: ABZR86_RS13370 - twin-arginine translocase subunit TatC, at 2,999,813 to 3,000,583
_RS13370
ABZR86_RS13375: ABZR86_RS13375 - Sec-independent protein translocase protein TatB, at 3,000,570 to 3,000,962
_RS13375
ABZR86_RS13380: ABZR86_RS13380 - Sec-independent protein translocase subunit TatA, at 3,000,984 to 3,001,220
_RS13380
ABZR86_RS13385: ABZR86_RS13385 - lipid-binding SYLF domain-containing protein, at 3,001,287 to 3,001,973
_RS13385
Position (kb)
2999
3000
3001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2998.964 kb on + strand, within ABZR86_RS13360 at 2998.964 kb on + strand, within ABZR86_RS13360 at 2998.964 kb on + strand, within ABZR86_RS13360 at 2998.964 kb on + strand, within ABZR86_RS13360 at 2998.964 kb on + strand, within ABZR86_RS13360 at 2998.965 kb on - strand, within ABZR86_RS13360 at 2998.965 kb on - strand, within ABZR86_RS13360 at 2998.965 kb on - strand, within ABZR86_RS13360 at 2998.965 kb on - strand, within ABZR86_RS13360 at 2999.213 kb on + strand at 2999.213 kb on + strand at 2999.213 kb on + strand at 2999.214 kb on - strand at 2999.214 kb on - strand at 2999.214 kb on - strand at 2999.216 kb on + strand at 2999.216 kb on + strand at 2999.278 kb on + strand at 2999.278 kb on + strand at 2999.278 kb on + strand at 2999.278 kb on + strand at 2999.279 kb on - strand at 2999.279 kb on - strand at 2999.279 kb on - strand at 2999.365 kb on + strand at 2999.365 kb on + strand at 2999.366 kb on - strand at 2999.366 kb on - strand at 2999.408 kb on + strand at 2999.468 kb on - strand, within ABZR86_RS13365 at 2999.468 kb on - strand, within ABZR86_RS13365 at 2999.468 kb on - strand, within ABZR86_RS13365 at 2999.468 kb on - strand, within ABZR86_RS13365 at 2999.468 kb on - strand, within ABZR86_RS13365 at 2999.584 kb on + strand, within ABZR86_RS13365 at 2999.584 kb on + strand, within ABZR86_RS13365 at 2999.584 kb on + strand, within ABZR86_RS13365 at 2999.584 kb on + strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.585 kb on - strand, within ABZR86_RS13365 at 2999.617 kb on + strand, within ABZR86_RS13365 at 2999.617 kb on + strand, within ABZR86_RS13365 at 2999.617 kb on + strand, within ABZR86_RS13365 at 2999.617 kb on + strand, within ABZR86_RS13365 at 2999.618 kb on - strand, within ABZR86_RS13365 at 2999.681 kb on - strand, within ABZR86_RS13365 at 2999.743 kb on + strand, within ABZR86_RS13365 at 2999.759 kb on + strand, within ABZR86_RS13365 at 2999.877 kb on + strand at 3000.093 kb on + strand, within ABZR86_RS13370 at 3000.094 kb on - strand, within ABZR86_RS13370 at 3000.094 kb on - strand, within ABZR86_RS13370 at 3000.177 kb on + strand, within ABZR86_RS13370 at 3000.178 kb on - strand, within ABZR86_RS13370 at 3000.198 kb on + strand, within ABZR86_RS13370 at 3000.199 kb on - strand, within ABZR86_RS13370 at 3000.199 kb on - strand, within ABZR86_RS13370 at 3000.255 kb on + strand, within ABZR86_RS13370 at 3000.255 kb on + strand, within ABZR86_RS13370 at 3000.255 kb on + strand, within ABZR86_RS13370 at 3000.255 kb on + strand, within ABZR86_RS13370 at 3000.255 kb on + strand, within ABZR86_RS13370 at 3000.255 kb on + strand, within ABZR86_RS13370 at 3000.256 kb on - strand, within ABZR86_RS13370 at 3000.256 kb on - strand, within ABZR86_RS13370 at 3000.288 kb on + strand, within ABZR86_RS13370 at 3000.288 kb on + strand, within ABZR86_RS13370 at 3000.288 kb on + strand, within ABZR86_RS13370 at 3000.289 kb on - strand, within ABZR86_RS13370 at 3000.289 kb on - strand, within ABZR86_RS13370 at 3000.325 kb on - strand, within ABZR86_RS13370 at 3000.325 kb on - strand, within ABZR86_RS13370 at 3000.325 kb on - strand, within ABZR86_RS13370 at 3000.325 kb on - strand, within ABZR86_RS13370 at 3000.325 kb on - strand, within ABZR86_RS13370 at 3000.429 kb on + strand, within ABZR86_RS13370 at 3000.985 kb on + strand at 3000.986 kb on - strand at 3000.986 kb on - strand at 3000.986 kb on - strand at 3000.986 kb on - strand at 3001.148 kb on + strand, within ABZR86_RS13380 at 3001.199 kb on + strand at 3001.300 kb on + strand at 3001.300 kb on + strand at 3001.301 kb on - strand at 3001.301 kb on - strand at 3001.301 kb on - strand at 3001.301 kb on - strand at 3001.301 kb on - strand at 3001.393 kb on + strand, within ABZR86_RS13385 at 3001.393 kb on + strand, within ABZR86_RS13385 at 3001.394 kb on - strand, within ABZR86_RS13385 at 3001.414 kb on + strand, within ABZR86_RS13385 at 3001.414 kb on + strand, within ABZR86_RS13385 at 3001.415 kb on - strand, within ABZR86_RS13385 at 3001.415 kb on - strand, within ABZR86_RS13385 at 3001.415 kb on - strand, within ABZR86_RS13385 at 3001.415 kb on - strand, within ABZR86_RS13385 at 3001.415 kb on - strand, within ABZR86_RS13385 at 3001.415 kb on - strand, within ABZR86_RS13385 at 3001.477 kb on + strand, within ABZR86_RS13385 at 3001.478 kb on - strand, within ABZR86_RS13385 at 3001.478 kb on - strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.483 kb on + strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385 at 3001.484 kb on - strand, within ABZR86_RS13385
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 2,998,964 + ABZR86_RS13360 0.85 -0.1 2,998,964 + ABZR86_RS13360 0.85 -0.4 2,998,964 + ABZR86_RS13360 0.85 -0.5 2,998,964 + ABZR86_RS13360 0.85 +1.1 2,998,964 + ABZR86_RS13360 0.85 -0.1 2,998,965 - ABZR86_RS13360 0.85 +0.1 2,998,965 - ABZR86_RS13360 0.85 +0.5 2,998,965 - ABZR86_RS13360 0.85 -0.2 2,998,965 - ABZR86_RS13360 0.85 -0.1 2,999,213 + -1.5 2,999,213 + -0.6 2,999,213 + -0.3 2,999,214 - -0.1 2,999,214 - -1.1 2,999,214 - -0.1 2,999,216 + -0.2 2,999,216 + -0.5 2,999,278 + +0.0 2,999,278 + +0.2 2,999,278 + +0.3 2,999,278 + +4.9 2,999,279 - +0.3 2,999,279 - -0.0 2,999,279 - -0.4 2,999,365 + +0.4 2,999,365 + -0.2 2,999,366 - -0.7 2,999,366 - -0.1 2,999,408 + +0.8 2,999,468 - ABZR86_RS13365 0.23 -0.4 2,999,468 - ABZR86_RS13365 0.23 -0.4 2,999,468 - ABZR86_RS13365 0.23 -0.1 2,999,468 - ABZR86_RS13365 0.23 +0.6 2,999,468 - ABZR86_RS13365 0.23 +0.4 2,999,584 + ABZR86_RS13365 0.49 -0.1 2,999,584 + ABZR86_RS13365 0.49 -0.2 2,999,584 + ABZR86_RS13365 0.49 -0.3 2,999,584 + ABZR86_RS13365 0.49 -1.1 2,999,585 - ABZR86_RS13365 0.49 +0.1 2,999,585 - ABZR86_RS13365 0.49 -0.4 2,999,585 - ABZR86_RS13365 0.49 -1.0 2,999,585 - ABZR86_RS13365 0.49 +0.2 2,999,585 - ABZR86_RS13365 0.49 -0.6 2,999,585 - ABZR86_RS13365 0.49 -0.8 2,999,585 - ABZR86_RS13365 0.49 +0.4 2,999,585 - ABZR86_RS13365 0.49 -0.3 2,999,617 + ABZR86_RS13365 0.56 -0.5 2,999,617 + ABZR86_RS13365 0.56 +0.2 2,999,617 + ABZR86_RS13365 0.56 -0.9 2,999,617 + ABZR86_RS13365 0.56 +0.6 2,999,618 - ABZR86_RS13365 0.56 -0.5 2,999,681 - ABZR86_RS13365 0.70 +0.1 2,999,743 + ABZR86_RS13365 0.84 -0.7 2,999,759 + ABZR86_RS13365 0.87 +0.5 2,999,877 + -0.4 3,000,093 + ABZR86_RS13370 0.36 -0.4 3,000,094 - ABZR86_RS13370 0.36 -1.0 3,000,094 - ABZR86_RS13370 0.36 -1.1 3,000,177 + ABZR86_RS13370 0.47 -0.9 3,000,178 - ABZR86_RS13370 0.47 -1.7 3,000,198 + ABZR86_RS13370 0.50 +1.0 3,000,199 - ABZR86_RS13370 0.50 -1.5 3,000,199 - ABZR86_RS13370 0.50 -1.0 3,000,255 + ABZR86_RS13370 0.57 -0.9 3,000,255 + ABZR86_RS13370 0.57 -1.9 3,000,255 + ABZR86_RS13370 0.57 +0.1 3,000,255 + ABZR86_RS13370 0.57 -1.2 3,000,255 + ABZR86_RS13370 0.57 -0.7 3,000,255 + ABZR86_RS13370 0.57 -0.1 3,000,256 - ABZR86_RS13370 0.57 -1.0 3,000,256 - ABZR86_RS13370 0.57 +0.7 3,000,288 + ABZR86_RS13370 0.62 +0.6 3,000,288 + ABZR86_RS13370 0.62 +0.2 3,000,288 + ABZR86_RS13370 0.62 -1.6 3,000,289 - ABZR86_RS13370 0.62 -0.4 3,000,289 - ABZR86_RS13370 0.62 -1.3 3,000,325 - ABZR86_RS13370 0.66 -0.1 3,000,325 - ABZR86_RS13370 0.66 -1.0 3,000,325 - ABZR86_RS13370 0.66 -3.1 3,000,325 - ABZR86_RS13370 0.66 -1.9 3,000,325 - ABZR86_RS13370 0.66 -2.3 3,000,429 + ABZR86_RS13370 0.80 -1.1 3,000,985 + -1.1 3,000,986 - +0.1 3,000,986 - -0.3 3,000,986 - -0.0 3,000,986 - +0.2 3,001,148 + ABZR86_RS13380 0.69 -0.9 3,001,199 + -0.6 3,001,300 + -0.3 3,001,300 + +0.7 3,001,301 - +0.8 3,001,301 - +0.0 3,001,301 - +0.5 3,001,301 - -1.1 3,001,301 - +0.3 3,001,393 + ABZR86_RS13385 0.15 +0.1 3,001,393 + ABZR86_RS13385 0.15 -1.9 3,001,394 - ABZR86_RS13385 0.16 -0.3 3,001,414 + ABZR86_RS13385 0.18 +0.1 3,001,414 + ABZR86_RS13385 0.18 -1.0 3,001,415 - ABZR86_RS13385 0.19 -0.2 3,001,415 - ABZR86_RS13385 0.19 +0.7 3,001,415 - ABZR86_RS13385 0.19 -1.6 3,001,415 - ABZR86_RS13385 0.19 -1.0 3,001,415 - ABZR86_RS13385 0.19 -0.1 3,001,415 - ABZR86_RS13385 0.19 +0.3 3,001,477 + ABZR86_RS13385 0.28 +0.8 3,001,478 - ABZR86_RS13385 0.28 +0.2 3,001,478 - ABZR86_RS13385 0.28 -0.5 3,001,483 + ABZR86_RS13385 0.29 -0.2 3,001,483 + ABZR86_RS13385 0.29 +0.3 3,001,483 + ABZR86_RS13385 0.29 -0.3 3,001,483 + ABZR86_RS13385 0.29 +0.5 3,001,483 + ABZR86_RS13385 0.29 +0.8 3,001,483 + ABZR86_RS13385 0.29 +0.0 3,001,483 + ABZR86_RS13385 0.29 -1.0 3,001,484 - ABZR86_RS13385 0.29 -0.6 3,001,484 - ABZR86_RS13385 0.29 -0.4 3,001,484 - ABZR86_RS13385 0.29 -0.7 3,001,484 - ABZR86_RS13385 0.29 -0.6 3,001,484 - ABZR86_RS13385 0.29 +0.5 3,001,484 - ABZR86_RS13385 0.29 +0.5 3,001,484 - ABZR86_RS13385 0.29 +0.2 3,001,484 - ABZR86_RS13385 0.29 +0.2 3,001,484 - ABZR86_RS13385 0.29 -1.2 3,001,484 - ABZR86_RS13385 0.29 +0.1 3,001,484 - ABZR86_RS13385 0.29 -0.4
Or see this region's nucleotide sequence