Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS11870 and ABZR86_RS11875 are separated by 52 nucleotides ABZR86_RS11875 and ABZR86_RS11880 are separated by 58 nucleotides ABZR86_RS11880 and ABZR86_RS11885 overlap by 4 nucleotides ABZR86_RS11885 and ABZR86_RS11890 are separated by 95 nucleotides
ABZR86_RS11870: ABZR86_RS11870 - phospholipid-binding protein MlaC, at 2,662,447 to 2,663,115
_RS11870
ABZR86_RS11875: ABZR86_RS11875 - outer membrane lipid asymmetry maintenance protein MlaD, at 2,663,168 to 2,664,040
_RS11875
ABZR86_RS11880: ABZR86_RS11880 - lipid asymmetry maintenance ABC transporter permease subunit MlaE, at 2,664,099 to 2,664,866
_RS11880
ABZR86_RS11885: ABZR86_RS11885 - ATP-binding cassette domain-containing protein, at 2,664,863 to 2,665,696
_RS11885
ABZR86_RS11890: ABZR86_RS11890 - guanylate kinase, at 2,665,792 to 2,666,445
_RS11890
Position (kb)
2664
2665 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2663.349 kb on + strand, within ABZR86_RS11875 at 2663.349 kb on + strand, within ABZR86_RS11875 at 2663.350 kb on - strand, within ABZR86_RS11875 at 2663.350 kb on - strand, within ABZR86_RS11875 at 2663.412 kb on + strand, within ABZR86_RS11875 at 2663.562 kb on + strand, within ABZR86_RS11875 at 2663.563 kb on - strand, within ABZR86_RS11875 at 2663.563 kb on - strand, within ABZR86_RS11875 at 2663.686 kb on + strand, within ABZR86_RS11875 at 2663.687 kb on - strand, within ABZR86_RS11875 at 2663.687 kb on - strand, within ABZR86_RS11875 at 2663.687 kb on - strand, within ABZR86_RS11875 at 2663.687 kb on - strand, within ABZR86_RS11875 at 2663.691 kb on + strand, within ABZR86_RS11875 at 2663.691 kb on + strand, within ABZR86_RS11875 at 2663.706 kb on + strand, within ABZR86_RS11875 at 2663.706 kb on + strand, within ABZR86_RS11875 at 2663.707 kb on - strand, within ABZR86_RS11875 at 2663.707 kb on - strand, within ABZR86_RS11875 at 2663.707 kb on - strand, within ABZR86_RS11875 at 2663.770 kb on - strand, within ABZR86_RS11875 at 2663.770 kb on - strand, within ABZR86_RS11875 at 2663.770 kb on - strand, within ABZR86_RS11875 at 2663.918 kb on - strand, within ABZR86_RS11875 at 2663.918 kb on - strand, within ABZR86_RS11875 at 2663.918 kb on - strand, within ABZR86_RS11875 at 2663.919 kb on + strand, within ABZR86_RS11875 at 2663.919 kb on + strand, within ABZR86_RS11875 at 2663.920 kb on - strand, within ABZR86_RS11875 at 2663.920 kb on - strand, within ABZR86_RS11875 at 2663.920 kb on - strand, within ABZR86_RS11875 at 2663.971 kb on - strand at 2664.021 kb on + strand at 2664.021 kb on + strand at 2664.075 kb on + strand at 2664.075 kb on + strand at 2664.075 kb on + strand at 2664.076 kb on - strand at 2664.076 kb on - strand at 2664.076 kb on - strand at 2664.087 kb on + strand at 2664.088 kb on - strand at 2664.088 kb on - strand at 2664.178 kb on + strand, within ABZR86_RS11880 at 2664.179 kb on - strand, within ABZR86_RS11880 at 2664.179 kb on - strand, within ABZR86_RS11880 at 2664.211 kb on + strand, within ABZR86_RS11880 at 2664.592 kb on + strand, within ABZR86_RS11880 at 2664.592 kb on + strand, within ABZR86_RS11880 at 2664.898 kb on - strand at 2665.032 kb on + strand, within ABZR86_RS11885 at 2665.032 kb on + strand, within ABZR86_RS11885 at 2665.032 kb on + strand, within ABZR86_RS11885 at 2665.033 kb on - strand, within ABZR86_RS11885 at 2665.414 kb on - strand, within ABZR86_RS11885 at 2665.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 2,663,349 + ABZR86_RS11875 0.21 -4.6 2,663,349 + ABZR86_RS11875 0.21 -3.1 2,663,350 - ABZR86_RS11875 0.21 -0.3 2,663,350 - ABZR86_RS11875 0.21 -2.5 2,663,412 + ABZR86_RS11875 0.28 -1.6 2,663,562 + ABZR86_RS11875 0.45 -3.2 2,663,563 - ABZR86_RS11875 0.45 -1.1 2,663,563 - ABZR86_RS11875 0.45 -2.7 2,663,686 + ABZR86_RS11875 0.59 -2.7 2,663,687 - ABZR86_RS11875 0.59 -3.2 2,663,687 - ABZR86_RS11875 0.59 -3.0 2,663,687 - ABZR86_RS11875 0.59 -1.2 2,663,687 - ABZR86_RS11875 0.59 -0.7 2,663,691 + ABZR86_RS11875 0.60 -2.3 2,663,691 + ABZR86_RS11875 0.60 -2.8 2,663,706 + ABZR86_RS11875 0.62 -4.1 2,663,706 + ABZR86_RS11875 0.62 -1.6 2,663,707 - ABZR86_RS11875 0.62 -4.0 2,663,707 - ABZR86_RS11875 0.62 -3.4 2,663,707 - ABZR86_RS11875 0.62 -2.7 2,663,770 - ABZR86_RS11875 0.69 -2.7 2,663,770 - ABZR86_RS11875 0.69 -2.6 2,663,770 - ABZR86_RS11875 0.69 -2.4 2,663,918 - ABZR86_RS11875 0.86 -3.3 2,663,918 - ABZR86_RS11875 0.86 -1.1 2,663,918 - ABZR86_RS11875 0.86 -1.8 2,663,919 + ABZR86_RS11875 0.86 -2.3 2,663,919 + ABZR86_RS11875 0.86 -0.0 2,663,920 - ABZR86_RS11875 0.86 -0.6 2,663,920 - ABZR86_RS11875 0.86 -2.7 2,663,920 - ABZR86_RS11875 0.86 -2.9 2,663,971 - -3.3 2,664,021 + -1.6 2,664,021 + -2.4 2,664,075 + -1.4 2,664,075 + -3.2 2,664,075 + +1.6 2,664,076 - -0.1 2,664,076 - -0.7 2,664,076 - -0.4 2,664,087 + -1.9 2,664,088 - -0.6 2,664,088 - -1.5 2,664,178 + ABZR86_RS11880 0.10 -0.8 2,664,179 - ABZR86_RS11880 0.10 -1.2 2,664,179 - ABZR86_RS11880 0.10 -2.8 2,664,211 + ABZR86_RS11880 0.15 -3.3 2,664,592 + ABZR86_RS11880 0.64 -4.2 2,664,592 + ABZR86_RS11880 0.64 -3.0 2,664,898 - -0.6 2,665,032 + ABZR86_RS11885 0.20 -2.8 2,665,032 + ABZR86_RS11885 0.20 -3.5 2,665,032 + ABZR86_RS11885 0.20 -0.7 2,665,033 - ABZR86_RS11885 0.20 -2.5 2,665,414 - ABZR86_RS11885 0.66 -3.1 2,665,729 + -1.8
Or see this region's nucleotide sequence