Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS10835 and ABZR86_RS10840 are separated by 4 nucleotides ABZR86_RS10840 and ABZR86_RS10845 overlap by 4 nucleotides ABZR86_RS10845 and ABZR86_RS10850 are separated by 51 nucleotides
ABZR86_RS10835: ABZR86_RS10835 - potassium transporter Kup, at 2,404,337 to 2,406,238
_RS10835
ABZR86_RS10840: ABZR86_RS10840 - Holliday junction branch migration protein RuvA, at 2,406,243 to 2,406,836
_RS10840
ABZR86_RS10845: ABZR86_RS10845 - crossover junction endodeoxyribonuclease RuvC, at 2,406,833 to 2,407,351
_RS10845
ABZR86_RS10850: ABZR86_RS10850 - YebC/PmpR family DNA-binding transcriptional regulator, at 2,407,403 to 2,408,143
_RS10850
Position (kb)
2406
2407 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2406.154 kb on - strand at 2406.253 kb on - strand at 2406.686 kb on - strand, within ABZR86_RS10840 at 2406.730 kb on + strand, within ABZR86_RS10840 at 2406.731 kb on - strand, within ABZR86_RS10840 at 2406.731 kb on - strand, within ABZR86_RS10840 at 2406.761 kb on - strand, within ABZR86_RS10840 at 2406.963 kb on - strand, within ABZR86_RS10845 at 2407.305 kb on - strand at 2407.386 kb on + strand at 2407.386 kb on + strand at 2407.387 kb on - strand at 2407.387 kb on - strand at 2407.437 kb on + strand at 2407.438 kb on - strand at 2407.438 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 2,406,154 - +1.1 2,406,253 - -0.6 2,406,686 - ABZR86_RS10840 0.75 -1.1 2,406,730 + ABZR86_RS10840 0.82 -1.1 2,406,731 - ABZR86_RS10840 0.82 -1.8 2,406,731 - ABZR86_RS10840 0.82 -1.7 2,406,761 - ABZR86_RS10840 0.87 -3.6 2,406,963 - ABZR86_RS10845 0.25 -0.7 2,407,305 - -0.7 2,407,386 + -0.2 2,407,386 + +0.5 2,407,387 - -0.2 2,407,387 - -0.9 2,407,437 + -1.4 2,407,438 - -0.7 2,407,438 - -1.6
Or see this region's nucleotide sequence