Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08835 and ABZR86_RS08840 are separated by 122 nucleotides ABZR86_RS08840 and ABZR86_RS08845 are separated by 84 nucleotides ABZR86_RS08845 and ABZR86_RS08850 are separated by 75 nucleotides
ABZR86_RS08835: ABZR86_RS08835 - cytochrome c biogenesis protein CcsA, at 1,974,517 to 1,975,311
_RS08835
ABZR86_RS08840: ABZR86_RS08840 - glutathione S-transferase N-terminal domain-containing protein, at 1,975,434 to 1,976,042
_RS08840
ABZR86_RS08845: ABZR86_RS08845 - DNA repair protein RadA, at 1,976,127 to 1,977,506
_RS08845
ABZR86_RS08850: ABZR86_RS08850 - alanine racemase, at 1,977,582 to 1,978,670
_RS08850
Position (kb) 1976
1977
1978 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1975.309 kb on + strand at 1975.309 kb on + strand at 1975.309 kb on + strand at 1975.309 kb on + strand at 1975.309 kb on + strand at 1975.310 kb on - strand at 1975.310 kb on - strand at 1975.310 kb on - strand at 1975.310 kb on - strand at 1975.359 kb on - strand at 1975.397 kb on + strand at 1975.398 kb on - strand at 1975.418 kb on - strand at 1975.651 kb on + strand, within ABZR86_RS08840 at 1975.652 kb on - strand, within ABZR86_RS08840 at 1975.652 kb on - strand, within ABZR86_RS08840 at 1975.652 kb on - strand, within ABZR86_RS08840 at 1975.758 kb on + strand, within ABZR86_RS08840 at 1975.758 kb on + strand, within ABZR86_RS08840 at 1975.758 kb on + strand, within ABZR86_RS08840 at 1975.758 kb on + strand, within ABZR86_RS08840 at 1975.759 kb on - strand, within ABZR86_RS08840 at 1975.759 kb on - strand, within ABZR86_RS08840 at 1975.833 kb on + strand, within ABZR86_RS08840 at 1975.834 kb on - strand, within ABZR86_RS08840 at 1975.834 kb on - strand, within ABZR86_RS08840 at 1975.834 kb on - strand, within ABZR86_RS08840 at 1975.834 kb on - strand, within ABZR86_RS08840 at 1975.834 kb on - strand, within ABZR86_RS08840 at 1976.446 kb on + strand, within ABZR86_RS08845 at 1976.446 kb on + strand, within ABZR86_RS08845 at 1976.447 kb on - strand, within ABZR86_RS08845 at 1976.782 kb on + strand, within ABZR86_RS08845 at 1976.783 kb on - strand, within ABZR86_RS08845 at 1976.783 kb on - strand, within ABZR86_RS08845 at 1976.783 kb on - strand, within ABZR86_RS08845 at 1976.783 kb on - strand, within ABZR86_RS08845 at 1977.127 kb on + strand, within ABZR86_RS08845 at 1977.127 kb on + strand, within ABZR86_RS08845 at 1977.128 kb on - strand, within ABZR86_RS08845 at 1977.128 kb on - strand, within ABZR86_RS08845 at 1977.128 kb on - strand, within ABZR86_RS08845 at 1977.484 kb on + strand at 1977.485 kb on - strand at 1977.485 kb on - strand at 1977.540 kb on - strand at 1977.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,975,309 + +0.1 1,975,309 + -0.1 1,975,309 + -0.0 1,975,309 + +0.7 1,975,309 + -0.2 1,975,310 - +1.0 1,975,310 - +0.1 1,975,310 - +1.0 1,975,310 - -0.1 1,975,359 - -0.4 1,975,397 + +0.6 1,975,398 - -0.4 1,975,418 - -0.1 1,975,651 + ABZR86_RS08840 0.36 -0.2 1,975,652 - ABZR86_RS08840 0.36 -0.1 1,975,652 - ABZR86_RS08840 0.36 +0.3 1,975,652 - ABZR86_RS08840 0.36 -0.1 1,975,758 + ABZR86_RS08840 0.53 -0.1 1,975,758 + ABZR86_RS08840 0.53 +2.2 1,975,758 + ABZR86_RS08840 0.53 +0.7 1,975,758 + ABZR86_RS08840 0.53 +0.9 1,975,759 - ABZR86_RS08840 0.53 -0.2 1,975,759 - ABZR86_RS08840 0.53 +0.6 1,975,833 + ABZR86_RS08840 0.66 -1.2 1,975,834 - ABZR86_RS08840 0.66 -0.4 1,975,834 - ABZR86_RS08840 0.66 +1.0 1,975,834 - ABZR86_RS08840 0.66 +0.1 1,975,834 - ABZR86_RS08840 0.66 -0.1 1,975,834 - ABZR86_RS08840 0.66 +0.2 1,976,446 + ABZR86_RS08845 0.23 -1.3 1,976,446 + ABZR86_RS08845 0.23 -2.5 1,976,447 - ABZR86_RS08845 0.23 -1.2 1,976,782 + ABZR86_RS08845 0.47 -1.7 1,976,783 - ABZR86_RS08845 0.48 -2.5 1,976,783 - ABZR86_RS08845 0.48 -3.1 1,976,783 - ABZR86_RS08845 0.48 -1.7 1,976,783 - ABZR86_RS08845 0.48 -2.0 1,977,127 + ABZR86_RS08845 0.72 +0.7 1,977,127 + ABZR86_RS08845 0.72 -1.4 1,977,128 - ABZR86_RS08845 0.73 -3.4 1,977,128 - ABZR86_RS08845 0.73 -3.2 1,977,128 - ABZR86_RS08845 0.73 -2.0 1,977,484 + -1.4 1,977,485 - -1.6 1,977,485 - -2.2 1,977,540 - -0.1 1,977,540 - -1.6
Or see this region's nucleotide sequence