Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08435 and ABZR86_RS08440 are separated by 341 nucleotides ABZR86_RS08440 and ABZR86_RS08445 are separated by 108 nucleotides
ABZR86_RS08435: ABZR86_RS08435 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 1,881,999 to 1,884,608
_RS08435
ABZR86_RS08440: ABZR86_RS08440 - TerC family protein, at 1,884,950 to 1,885,636
_RS08440
ABZR86_RS08445: ABZR86_RS08445 - acetate kinase, at 1,885,745 to 1,887,133
_RS08445
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1884.503 kb on - strand at 1884.562 kb on + strand at 1884.608 kb on + strand at 1884.608 kb on + strand at 1884.608 kb on + strand at 1884.608 kb on + strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.609 kb on - strand at 1884.686 kb on - strand at 1884.686 kb on - strand at 1884.690 kb on + strand at 1884.690 kb on + strand at 1884.691 kb on - strand at 1884.733 kb on + strand at 1884.733 kb on + strand at 1884.780 kb on + strand at 1884.780 kb on + strand at 1884.780 kb on + strand at 1884.780 kb on + strand at 1884.780 kb on + strand at 1884.781 kb on - strand at 1884.942 kb on + strand at 1884.942 kb on + strand at 1884.943 kb on - strand at 1884.943 kb on - strand at 1884.943 kb on - strand at 1884.943 kb on - strand at 1884.943 kb on - strand at 1884.943 kb on - strand at 1884.943 kb on - strand at 1885.145 kb on + strand, within ABZR86_RS08440 at 1885.146 kb on - strand, within ABZR86_RS08440 at 1885.146 kb on - strand, within ABZR86_RS08440 at 1885.449 kb on - strand, within ABZR86_RS08440 at 1885.449 kb on - strand, within ABZR86_RS08440 at 1885.449 kb on - strand, within ABZR86_RS08440 at 1885.592 kb on + strand at 1885.592 kb on + strand at 1885.592 kb on + strand at 1885.592 kb on + strand at 1885.593 kb on - strand at 1885.749 kb on + strand at 1885.749 kb on + strand at 1885.749 kb on + strand at 1885.749 kb on + strand at 1885.749 kb on + strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1885.750 kb on - strand at 1886.094 kb on + strand, within ABZR86_RS08445 at 1886.133 kb on + strand, within ABZR86_RS08445 at 1886.134 kb on - strand, within ABZR86_RS08445 at 1886.298 kb on + strand, within ABZR86_RS08445 at 1886.298 kb on + strand, within ABZR86_RS08445 at 1886.298 kb on + strand, within ABZR86_RS08445 at 1886.299 kb on - strand, within ABZR86_RS08445 at 1886.299 kb on - strand, within ABZR86_RS08445 at 1886.299 kb on - strand, within ABZR86_RS08445 at 1886.301 kb on + strand, within ABZR86_RS08445 at 1886.335 kb on - strand, within ABZR86_RS08445 at 1886.448 kb on + strand, within ABZR86_RS08445 at 1886.449 kb on - strand, within ABZR86_RS08445 at 1886.449 kb on - strand, within ABZR86_RS08445 at 1886.449 kb on - strand, within ABZR86_RS08445 at 1886.577 kb on + strand, within ABZR86_RS08445 at 1886.622 kb on + strand, within ABZR86_RS08445
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,884,503 - +3.8 1,884,562 + +3.2 1,884,608 + +1.2 1,884,608 + +2.5 1,884,608 + +2.4 1,884,608 + +2.1 1,884,609 - +0.3 1,884,609 - -0.6 1,884,609 - +1.3 1,884,609 - +0.3 1,884,609 - -0.5 1,884,609 - +0.5 1,884,609 - -0.1 1,884,609 - +1.3 1,884,609 - +0.9 1,884,609 - -0.5 1,884,609 - +0.2 1,884,609 - +0.0 1,884,609 - +0.9 1,884,609 - +0.1 1,884,609 - -0.9 1,884,609 - +0.3 1,884,609 - +0.7 1,884,609 - +0.5 1,884,686 - +0.8 1,884,686 - +1.0 1,884,690 + +2.6 1,884,690 + +0.7 1,884,691 - +0.7 1,884,733 + -0.1 1,884,733 + +0.2 1,884,780 + +0.9 1,884,780 + -0.2 1,884,780 + -1.1 1,884,780 + -0.9 1,884,780 + +0.5 1,884,781 - +0.3 1,884,942 + +0.3 1,884,942 + +0.2 1,884,943 - +0.8 1,884,943 - -0.5 1,884,943 - +1.0 1,884,943 - +1.1 1,884,943 - -0.3 1,884,943 - -0.0 1,884,943 - +0.8 1,885,145 + ABZR86_RS08440 0.28 +1.8 1,885,146 - ABZR86_RS08440 0.29 +0.7 1,885,146 - ABZR86_RS08440 0.29 +3.2 1,885,449 - ABZR86_RS08440 0.73 -0.1 1,885,449 - ABZR86_RS08440 0.73 +1.4 1,885,449 - ABZR86_RS08440 0.73 +1.0 1,885,592 + -0.2 1,885,592 + +1.0 1,885,592 + +1.0 1,885,592 + -0.2 1,885,593 - +0.2 1,885,749 + -1.4 1,885,749 + -0.3 1,885,749 + +0.9 1,885,749 + +0.6 1,885,749 + +0.2 1,885,750 - -0.4 1,885,750 - -0.3 1,885,750 - +1.1 1,885,750 - +0.1 1,885,750 - +1.1 1,885,750 - -0.2 1,885,750 - -0.7 1,885,750 - +0.8 1,886,094 + ABZR86_RS08445 0.25 -1.1 1,886,133 + ABZR86_RS08445 0.28 +1.0 1,886,134 - ABZR86_RS08445 0.28 +1.1 1,886,298 + ABZR86_RS08445 0.40 +0.5 1,886,298 + ABZR86_RS08445 0.40 +0.6 1,886,298 + ABZR86_RS08445 0.40 +1.0 1,886,299 - ABZR86_RS08445 0.40 +0.3 1,886,299 - ABZR86_RS08445 0.40 +0.4 1,886,299 - ABZR86_RS08445 0.40 -1.7 1,886,301 + ABZR86_RS08445 0.40 -0.1 1,886,335 - ABZR86_RS08445 0.42 -0.2 1,886,448 + ABZR86_RS08445 0.51 +0.2 1,886,449 - ABZR86_RS08445 0.51 +2.0 1,886,449 - ABZR86_RS08445 0.51 +2.1 1,886,449 - ABZR86_RS08445 0.51 +0.1 1,886,577 + ABZR86_RS08445 0.60 -0.0 1,886,622 + ABZR86_RS08445 0.63 +0.4
Or see this region's nucleotide sequence