Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08425 and ABZR86_RS08430 are separated by 99 nucleotides ABZR86_RS08430 and ABZR86_RS08435 are separated by 2 nucleotides
ABZR86_RS08425: ABZR86_RS08425 - bifunctional 2-methylcitrate dehydratase/aconitate hydratase, at 1,879,246 to 1,880,697
_RS08425
ABZR86_RS08430: ABZR86_RS08430 - 2-methylaconitate cis-trans isomerase PrpF, at 1,880,797 to 1,881,996
_RS08430
ABZR86_RS08435: ABZR86_RS08435 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 1,881,999 to 1,884,608
_RS08435
Position (kb)
1880
1881
1882 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1879.830 kb on - strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.862 kb on + strand, within ABZR86_RS08425 at 1879.863 kb on - strand, within ABZR86_RS08425 at 1879.863 kb on - strand, within ABZR86_RS08425 at 1879.863 kb on - strand, within ABZR86_RS08425 at 1879.916 kb on + strand, within ABZR86_RS08425 at 1879.916 kb on + strand, within ABZR86_RS08425 at 1879.917 kb on - strand, within ABZR86_RS08425 at 1879.917 kb on - strand, within ABZR86_RS08425 at 1879.919 kb on + strand, within ABZR86_RS08425 at 1879.919 kb on + strand, within ABZR86_RS08425 at 1879.919 kb on + strand, within ABZR86_RS08425 at 1879.919 kb on + strand, within ABZR86_RS08425 at 1880.220 kb on - strand, within ABZR86_RS08425 at 1880.316 kb on - strand, within ABZR86_RS08425 at 1880.316 kb on - strand, within ABZR86_RS08425 at 1880.576 kb on + strand at 1880.576 kb on + strand at 1880.577 kb on - strand at 1880.577 kb on - strand at 1880.592 kb on - strand at 1880.592 kb on - strand at 1880.592 kb on - strand at 1880.592 kb on - strand at 1880.592 kb on - strand at 1880.627 kb on + strand at 1880.627 kb on + strand at 1880.627 kb on + strand at 1880.627 kb on + strand at 1880.628 kb on - strand at 1880.628 kb on - strand at 1880.628 kb on - strand at 1880.628 kb on - strand at 1880.637 kb on + strand at 1880.691 kb on + strand at 1881.024 kb on + strand, within ABZR86_RS08430 at 1881.186 kb on + strand, within ABZR86_RS08430 at 1881.186 kb on + strand, within ABZR86_RS08430 at 1881.290 kb on + strand, within ABZR86_RS08430 at 1881.726 kb on - strand, within ABZR86_RS08430 at 1881.944 kb on + strand at 1881.945 kb on - strand at 1881.945 kb on - strand at 1882.063 kb on + strand at 1882.370 kb on - strand, within ABZR86_RS08435 at 1882.414 kb on + strand, within ABZR86_RS08435 at 1882.415 kb on - strand, within ABZR86_RS08435 at 1882.415 kb on - strand, within ABZR86_RS08435 at 1882.415 kb on - strand, within ABZR86_RS08435 at 1882.415 kb on - strand, within ABZR86_RS08435 at 1882.579 kb on + strand, within ABZR86_RS08435 at 1882.621 kb on + strand, within ABZR86_RS08435 at 1882.754 kb on - strand, within ABZR86_RS08435 at 1882.771 kb on + strand, within ABZR86_RS08435 at 1882.771 kb on + strand, within ABZR86_RS08435 at 1882.771 kb on + strand, within ABZR86_RS08435 at 1882.772 kb on - strand, within ABZR86_RS08435 at 1882.849 kb on + strand, within ABZR86_RS08435 at 1882.850 kb on - strand, within ABZR86_RS08435
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,879,830 - ABZR86_RS08425 0.40 -1.1 1,879,862 + ABZR86_RS08425 0.42 +0.7 1,879,862 + ABZR86_RS08425 0.42 -0.7 1,879,862 + ABZR86_RS08425 0.42 +0.5 1,879,862 + ABZR86_RS08425 0.42 +0.9 1,879,862 + ABZR86_RS08425 0.42 -0.9 1,879,862 + ABZR86_RS08425 0.42 +0.2 1,879,862 + ABZR86_RS08425 0.42 -0.5 1,879,863 - ABZR86_RS08425 0.42 +0.0 1,879,863 - ABZR86_RS08425 0.42 +2.3 1,879,863 - ABZR86_RS08425 0.42 -0.2 1,879,916 + ABZR86_RS08425 0.46 +0.1 1,879,916 + ABZR86_RS08425 0.46 -0.5 1,879,917 - ABZR86_RS08425 0.46 -0.0 1,879,917 - ABZR86_RS08425 0.46 +0.1 1,879,919 + ABZR86_RS08425 0.46 +0.2 1,879,919 + ABZR86_RS08425 0.46 +0.3 1,879,919 + ABZR86_RS08425 0.46 -0.1 1,879,919 + ABZR86_RS08425 0.46 +0.6 1,880,220 - ABZR86_RS08425 0.67 +0.6 1,880,316 - ABZR86_RS08425 0.74 +0.1 1,880,316 - ABZR86_RS08425 0.74 +0.1 1,880,576 + -1.1 1,880,576 + -0.6 1,880,577 - +0.1 1,880,577 - -0.1 1,880,592 - +0.7 1,880,592 - -0.5 1,880,592 - +0.2 1,880,592 - +0.0 1,880,592 - +0.9 1,880,627 + +1.0 1,880,627 + -0.4 1,880,627 + +0.7 1,880,627 + +0.3 1,880,628 - -0.3 1,880,628 - -0.2 1,880,628 - -0.9 1,880,628 - +1.1 1,880,637 + +0.5 1,880,691 + -0.3 1,881,024 + ABZR86_RS08430 0.19 +2.3 1,881,186 + ABZR86_RS08430 0.32 +2.6 1,881,186 + ABZR86_RS08430 0.32 +3.0 1,881,290 + ABZR86_RS08430 0.41 +3.4 1,881,726 - ABZR86_RS08430 0.77 +2.5 1,881,944 + +3.4 1,881,945 - +2.9 1,881,945 - +2.7 1,882,063 + +2.8 1,882,370 - ABZR86_RS08435 0.14 +1.7 1,882,414 + ABZR86_RS08435 0.16 +4.0 1,882,415 - ABZR86_RS08435 0.16 +3.1 1,882,415 - ABZR86_RS08435 0.16 +2.8 1,882,415 - ABZR86_RS08435 0.16 +3.5 1,882,415 - ABZR86_RS08435 0.16 +1.7 1,882,579 + ABZR86_RS08435 0.22 +3.1 1,882,621 + ABZR86_RS08435 0.24 +3.2 1,882,754 - ABZR86_RS08435 0.29 +3.2 1,882,771 + ABZR86_RS08435 0.30 +2.9 1,882,771 + ABZR86_RS08435 0.30 +3.4 1,882,771 + ABZR86_RS08435 0.30 +2.5 1,882,772 - ABZR86_RS08435 0.30 +2.9 1,882,849 + ABZR86_RS08435 0.33 +2.2 1,882,850 - ABZR86_RS08435 0.33 +3.1
Or see this region's nucleotide sequence