Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08405 and ABZR86_RS08410 are separated by 0 nucleotides ABZR86_RS08410 and ABZR86_RS08415 are separated by 79 nucleotides ABZR86_RS08415 and ABZR86_RS08420 are separated by 321 nucleotides
ABZR86_RS08405: ABZR86_RS08405 - LysR family transcriptional regulator, at 1,874,789 to 1,875,658
_RS08405
ABZR86_RS08410: ABZR86_RS08410 - pseudouridine synthase, at 1,875,659 to 1,876,435
_RS08410
ABZR86_RS08415: ABZR86_RS08415 - OmpA family protein, at 1,876,515 to 1,877,621
_RS08415
ABZR86_RS08420: ABZR86_RS08420 - OmpA family protein, at 1,877,943 to 1,879,058
_RS08420
Position (kb)
1876
1877
1878 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1875.571 kb on + strand, within ABZR86_RS08405 at 1875.571 kb on + strand, within ABZR86_RS08405 at 1875.571 kb on + strand, within ABZR86_RS08405 at 1875.572 kb on - strand at 1875.572 kb on - strand at 1875.572 kb on - strand at 1875.572 kb on - strand at 1875.578 kb on + strand at 1875.578 kb on + strand at 1875.578 kb on + strand at 1875.578 kb on + strand at 1875.601 kb on + strand at 1875.633 kb on + strand at 1875.966 kb on + strand, within ABZR86_RS08410 at 1875.966 kb on + strand, within ABZR86_RS08410 at 1875.966 kb on + strand, within ABZR86_RS08410 at 1875.966 kb on + strand, within ABZR86_RS08410 at 1875.967 kb on - strand, within ABZR86_RS08410 at 1875.967 kb on - strand, within ABZR86_RS08410 at 1875.967 kb on - strand, within ABZR86_RS08410 at 1876.111 kb on - strand, within ABZR86_RS08410 at 1876.111 kb on - strand, within ABZR86_RS08410 at 1876.111 kb on - strand, within ABZR86_RS08410 at 1876.179 kb on + strand, within ABZR86_RS08410 at 1876.180 kb on - strand, within ABZR86_RS08410 at 1876.696 kb on + strand, within ABZR86_RS08415 at 1877.272 kb on + strand, within ABZR86_RS08415 at 1877.302 kb on + strand, within ABZR86_RS08415 at 1877.441 kb on - strand, within ABZR86_RS08415 at 1877.483 kb on - strand, within ABZR86_RS08415 at 1877.737 kb on - strand at 1877.737 kb on - strand at 1877.758 kb on + strand at 1877.758 kb on + strand at 1877.758 kb on + strand at 1877.771 kb on - strand at 1877.777 kb on + strand at 1877.777 kb on + strand at 1877.777 kb on + strand at 1877.778 kb on - strand at 1877.778 kb on - strand at 1877.790 kb on + strand at 1877.812 kb on - strand at 1877.875 kb on - strand at 1877.875 kb on - strand at 1877.875 kb on - strand at 1877.944 kb on + strand at 1877.945 kb on - strand at 1877.945 kb on - strand at 1877.945 kb on - strand at 1877.945 kb on - strand at 1878.080 kb on - strand, within ABZR86_RS08420 at 1878.080 kb on - strand, within ABZR86_RS08420 at 1878.135 kb on - strand, within ABZR86_RS08420 at 1878.149 kb on + strand, within ABZR86_RS08420 at 1878.362 kb on + strand, within ABZR86_RS08420 at 1878.568 kb on + strand, within ABZR86_RS08420
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,875,571 + ABZR86_RS08405 0.90 +0.8 1,875,571 + ABZR86_RS08405 0.90 -0.2 1,875,571 + ABZR86_RS08405 0.90 +0.2 1,875,572 - -2.0 1,875,572 - +0.4 1,875,572 - -0.6 1,875,572 - +0.0 1,875,578 + +0.5 1,875,578 + -0.3 1,875,578 + +0.6 1,875,578 + -1.1 1,875,601 + -0.3 1,875,633 + -2.0 1,875,966 + ABZR86_RS08410 0.40 +0.5 1,875,966 + ABZR86_RS08410 0.40 +0.0 1,875,966 + ABZR86_RS08410 0.40 -0.4 1,875,966 + ABZR86_RS08410 0.40 -0.4 1,875,967 - ABZR86_RS08410 0.40 -0.7 1,875,967 - ABZR86_RS08410 0.40 -0.6 1,875,967 - ABZR86_RS08410 0.40 -0.2 1,876,111 - ABZR86_RS08410 0.58 -0.0 1,876,111 - ABZR86_RS08410 0.58 -0.8 1,876,111 - ABZR86_RS08410 0.58 -1.1 1,876,179 + ABZR86_RS08410 0.67 -1.1 1,876,180 - ABZR86_RS08410 0.67 +0.2 1,876,696 + ABZR86_RS08415 0.16 -2.3 1,877,272 + ABZR86_RS08415 0.68 +1.6 1,877,302 + ABZR86_RS08415 0.71 -0.7 1,877,441 - ABZR86_RS08415 0.84 -2.8 1,877,483 - ABZR86_RS08415 0.87 +0.4 1,877,737 - -0.7 1,877,737 - -0.6 1,877,758 + +0.2 1,877,758 + +0.1 1,877,758 + -1.5 1,877,771 - -0.8 1,877,777 + -1.3 1,877,777 + -1.8 1,877,777 + -2.7 1,877,778 - -0.3 1,877,778 - +1.9 1,877,790 + -1.1 1,877,812 - -2.7 1,877,875 - -0.6 1,877,875 - +0.6 1,877,875 - +0.7 1,877,944 + +0.2 1,877,945 - -0.5 1,877,945 - +0.2 1,877,945 - -0.1 1,877,945 - -0.1 1,878,080 - ABZR86_RS08420 0.12 +0.7 1,878,080 - ABZR86_RS08420 0.12 -0.3 1,878,135 - ABZR86_RS08420 0.17 -0.5 1,878,149 + ABZR86_RS08420 0.18 +0.6 1,878,362 + ABZR86_RS08420 0.38 +0.2 1,878,568 + ABZR86_RS08420 0.56 -1.3
Or see this region's nucleotide sequence