Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS07520 and ABZR86_RS07525 are separated by 46 nucleotides ABZR86_RS07525 and ABZR86_RS07530 are separated by 8 nucleotides ABZR86_RS07530 and ABZR86_RS07535 are separated by 5 nucleotides
ABZR86_RS07520: ABZR86_RS07520 - thiamine pyrophosphate-dependent enzyme, at 1,657,361 to 1,659,628
_RS07520
ABZR86_RS07525: ABZR86_RS07525 - hypothetical protein, at 1,659,675 to 1,660,151
_RS07525
ABZR86_RS07530: ABZR86_RS07530 - GNAT family N-acetyltransferase, at 1,660,160 to 1,660,720
_RS07530
ABZR86_RS07535: ABZR86_RS07535 - acyl-CoA dehydrogenase family protein, at 1,660,726 to 1,661,895
_RS07535
Position (kb)
1659
1660
1661 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1658.799 kb on + strand, within ABZR86_RS07520 at 1658.921 kb on + strand, within ABZR86_RS07520 at 1658.952 kb on + strand, within ABZR86_RS07520 at 1658.953 kb on - strand, within ABZR86_RS07520 at 1658.953 kb on - strand, within ABZR86_RS07520 at 1659.294 kb on + strand, within ABZR86_RS07520 at 1659.295 kb on - strand, within ABZR86_RS07520 at 1659.375 kb on + strand, within ABZR86_RS07520 at 1659.375 kb on + strand, within ABZR86_RS07520 at 1659.375 kb on + strand, within ABZR86_RS07520 at 1659.376 kb on - strand, within ABZR86_RS07520 at 1659.376 kb on - strand, within ABZR86_RS07520 at 1659.376 kb on - strand, within ABZR86_RS07520 at 1659.376 kb on - strand, within ABZR86_RS07520 at 1659.542 kb on + strand at 1659.542 kb on + strand at 1659.542 kb on + strand at 1659.542 kb on + strand at 1659.543 kb on - strand at 1659.673 kb on + strand at 1659.674 kb on - strand at 1659.807 kb on + strand, within ABZR86_RS07525 at 1659.807 kb on + strand, within ABZR86_RS07525 at 1659.807 kb on + strand, within ABZR86_RS07525 at 1659.816 kb on + strand, within ABZR86_RS07525 at 1659.816 kb on + strand, within ABZR86_RS07525 at 1659.816 kb on + strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.817 kb on - strand, within ABZR86_RS07525 at 1659.949 kb on + strand, within ABZR86_RS07525 at 1659.949 kb on + strand, within ABZR86_RS07525 at 1659.949 kb on + strand, within ABZR86_RS07525 at 1659.950 kb on - strand, within ABZR86_RS07525 at 1659.950 kb on - strand, within ABZR86_RS07525 at 1659.950 kb on - strand, within ABZR86_RS07525 at 1660.386 kb on + strand, within ABZR86_RS07530 at 1660.387 kb on - strand, within ABZR86_RS07530 at 1660.593 kb on + strand, within ABZR86_RS07530 at 1660.593 kb on + strand, within ABZR86_RS07530 at 1660.594 kb on - strand, within ABZR86_RS07530 at 1660.594 kb on - strand, within ABZR86_RS07530 at 1660.705 kb on + strand at 1660.793 kb on + strand at 1660.793 kb on + strand at 1660.793 kb on + strand at 1660.794 kb on - strand at 1660.794 kb on - strand at 1660.846 kb on - strand, within ABZR86_RS07535 at 1661.087 kb on + strand, within ABZR86_RS07535 at 1661.088 kb on - strand, within ABZR86_RS07535
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,658,799 + ABZR86_RS07520 0.63 +0.6 1,658,921 + ABZR86_RS07520 0.69 -0.0 1,658,952 + ABZR86_RS07520 0.70 -0.4 1,658,953 - ABZR86_RS07520 0.70 -0.8 1,658,953 - ABZR86_RS07520 0.70 +0.1 1,659,294 + ABZR86_RS07520 0.85 -0.2 1,659,295 - ABZR86_RS07520 0.85 +0.0 1,659,375 + ABZR86_RS07520 0.89 -0.2 1,659,375 + ABZR86_RS07520 0.89 +1.0 1,659,375 + ABZR86_RS07520 0.89 +0.9 1,659,376 - ABZR86_RS07520 0.89 -0.4 1,659,376 - ABZR86_RS07520 0.89 -0.6 1,659,376 - ABZR86_RS07520 0.89 -0.5 1,659,376 - ABZR86_RS07520 0.89 -0.5 1,659,542 + -0.0 1,659,542 + -0.1 1,659,542 + +0.1 1,659,542 + +0.2 1,659,543 - +0.3 1,659,673 + -0.9 1,659,674 - -0.7 1,659,807 + ABZR86_RS07525 0.28 +0.2 1,659,807 + ABZR86_RS07525 0.28 -0.9 1,659,807 + ABZR86_RS07525 0.28 -0.2 1,659,816 + ABZR86_RS07525 0.30 -0.1 1,659,816 + ABZR86_RS07525 0.30 -0.6 1,659,816 + ABZR86_RS07525 0.30 +1.9 1,659,817 - ABZR86_RS07525 0.30 -0.0 1,659,817 - ABZR86_RS07525 0.30 -0.2 1,659,817 - ABZR86_RS07525 0.30 +0.1 1,659,817 - ABZR86_RS07525 0.30 -0.3 1,659,817 - ABZR86_RS07525 0.30 +0.4 1,659,817 - ABZR86_RS07525 0.30 -1.3 1,659,817 - ABZR86_RS07525 0.30 +0.2 1,659,949 + ABZR86_RS07525 0.57 +0.2 1,659,949 + ABZR86_RS07525 0.57 +0.4 1,659,949 + ABZR86_RS07525 0.57 -0.1 1,659,950 - ABZR86_RS07525 0.58 -0.2 1,659,950 - ABZR86_RS07525 0.58 +2.5 1,659,950 - ABZR86_RS07525 0.58 +0.5 1,660,386 + ABZR86_RS07530 0.40 -0.7 1,660,387 - ABZR86_RS07530 0.40 -1.1 1,660,593 + ABZR86_RS07530 0.77 +0.5 1,660,593 + ABZR86_RS07530 0.77 -0.9 1,660,594 - ABZR86_RS07530 0.77 -0.5 1,660,594 - ABZR86_RS07530 0.77 +0.0 1,660,705 + +0.2 1,660,793 + -0.4 1,660,793 + -2.1 1,660,793 + -0.7 1,660,794 - +0.7 1,660,794 - -0.2 1,660,846 - ABZR86_RS07535 0.10 -0.1 1,661,087 + ABZR86_RS07535 0.31 -0.6 1,661,088 - ABZR86_RS07535 0.31 +0.1
Or see this region's nucleotide sequence