Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05680 and ABZR86_RS05685 are separated by 79 nucleotides ABZR86_RS05685 and ABZR86_RS05690 overlap by 4 nucleotides ABZR86_RS05690 and ABZR86_RS05695 overlap by 8 nucleotides ABZR86_RS05695 and ABZR86_RS05700 are separated by 80 nucleotides
ABZR86_RS05680: ABZR86_RS05680 - ferric iron uptake transcriptional regulator, at 1,285,324 to 1,285,743
_RS05680
ABZR86_RS05685: ABZR86_RS05685 - outer membrane protein assembly factor BamE, at 1,285,823 to 1,286,284
_RS05685
ABZR86_RS05690: ABZR86_RS05690 - RnfH family protein, at 1,286,281 to 1,286,562
_RS05690
ABZR86_RS05695: ABZR86_RS05695 - type II toxin-antitoxin system RatA family toxin, at 1,286,555 to 1,286,986
_RS05695
ABZR86_RS05700: ABZR86_RS05700 - SsrA-binding protein SmpB, at 1,287,067 to 1,287,555
_RS05700
Position (kb)
1286
1287 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1285.782 kb on - strand at 1285.786 kb on + strand at 1285.786 kb on + strand at 1285.786 kb on + strand at 1285.786 kb on + strand at 1285.786 kb on + strand at 1285.786 kb on + strand at 1285.786 kb on + strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.787 kb on - strand at 1285.892 kb on + strand, within ABZR86_RS05685 at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.282 kb on + strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.283 kb on - strand at 1286.532 kb on - strand, within ABZR86_RS05690 at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.562 kb on + strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1286.563 kb on - strand at 1287.078 kb on + strand at 1287.139 kb on + strand, within ABZR86_RS05700 at 1287.140 kb on - strand, within ABZR86_RS05700 at 1287.232 kb on + strand, within ABZR86_RS05700 at 1287.232 kb on + strand, within ABZR86_RS05700 at 1287.233 kb on - strand, within ABZR86_RS05700 at 1287.418 kb on + strand, within ABZR86_RS05700 at 1287.418 kb on + strand, within ABZR86_RS05700 at 1287.418 kb on + strand, within ABZR86_RS05700 at 1287.418 kb on + strand, within ABZR86_RS05700 at 1287.419 kb on - strand, within ABZR86_RS05700 at 1287.419 kb on - strand, within ABZR86_RS05700
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,285,782 - +2.4 1,285,786 + +0.2 1,285,786 + -1.1 1,285,786 + +1.2 1,285,786 + -0.5 1,285,786 + -0.3 1,285,786 + -0.6 1,285,786 + +0.2 1,285,787 - -0.6 1,285,787 - +0.9 1,285,787 - -0.0 1,285,787 - +1.1 1,285,787 - -0.9 1,285,787 - -0.4 1,285,787 - -1.3 1,285,787 - -0.2 1,285,892 + ABZR86_RS05685 0.15 -0.4 1,286,282 + -0.3 1,286,282 + -0.2 1,286,282 + -0.9 1,286,282 + -0.1 1,286,282 + -0.3 1,286,282 + -0.3 1,286,282 + -0.0 1,286,282 + -0.4 1,286,282 + -0.1 1,286,282 + -1.2 1,286,282 + +0.8 1,286,282 + +0.2 1,286,283 - -0.4 1,286,283 - -1.3 1,286,283 - -0.9 1,286,283 - -1.5 1,286,283 - -0.3 1,286,283 - +0.8 1,286,283 - -0.2 1,286,283 - +0.2 1,286,283 - -0.9 1,286,532 - ABZR86_RS05690 0.89 -0.7 1,286,562 + +0.0 1,286,562 + +1.5 1,286,562 + -0.0 1,286,562 + -1.2 1,286,562 + -0.5 1,286,562 + +0.4 1,286,562 + +1.4 1,286,562 + -0.3 1,286,562 + -0.1 1,286,562 + -0.8 1,286,562 + -1.4 1,286,562 + -0.1 1,286,563 - +0.5 1,286,563 - +0.1 1,286,563 - +0.3 1,286,563 - -0.4 1,286,563 - -0.7 1,286,563 - -0.7 1,286,563 - +1.0 1,286,563 - -0.4 1,286,563 - +0.6 1,286,563 - -1.0 1,286,563 - -0.0 1,286,563 - -1.4 1,287,078 + +1.6 1,287,139 + ABZR86_RS05700 0.15 +1.3 1,287,140 - ABZR86_RS05700 0.15 +2.0 1,287,232 + ABZR86_RS05700 0.34 +2.6 1,287,232 + ABZR86_RS05700 0.34 -0.5 1,287,233 - ABZR86_RS05700 0.34 +1.7 1,287,418 + ABZR86_RS05700 0.72 +2.0 1,287,418 + ABZR86_RS05700 0.72 +3.0 1,287,418 + ABZR86_RS05700 0.72 +2.7 1,287,418 + ABZR86_RS05700 0.72 +1.7 1,287,419 - ABZR86_RS05700 0.72 +1.1 1,287,419 - ABZR86_RS05700 0.72 +2.6
Or see this region's nucleotide sequence