Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05565 and ABZR86_RS05570 are separated by 155 nucleotides ABZR86_RS05570 and ABZR86_RS05575 are separated by 107 nucleotides ABZR86_RS05575 and ABZR86_RS05580 are separated by 66 nucleotides
ABZR86_RS05565: ABZR86_RS05565 - 30S ribosomal protein S1, at 1,257,552 to 1,259,225
_RS05565
ABZR86_RS05570: ABZR86_RS05570 - (d)CMP kinase, at 1,259,381 to 1,260,073
_RS05570
ABZR86_RS05575: ABZR86_RS05575 - acyl-CoA-binding protein, at 1,260,181 to 1,260,441
_RS05575
ABZR86_RS05580: ABZR86_RS05580 - NADPH-dependent 2,4-dienoyl-CoA reductase, at 1,260,508 to 1,262,520
_RS05580
Position (kb)
1259
1260
1261 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1259.316 kb on - strand at 1259.316 kb on - strand at 1259.316 kb on - strand at 1259.316 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.375 kb on - strand at 1259.453 kb on - strand, within ABZR86_RS05570 at 1259.516 kb on - strand, within ABZR86_RS05570 at 1259.516 kb on - strand, within ABZR86_RS05570 at 1259.587 kb on - strand, within ABZR86_RS05570 at 1259.634 kb on + strand, within ABZR86_RS05570 at 1259.634 kb on + strand, within ABZR86_RS05570 at 1259.634 kb on + strand, within ABZR86_RS05570 at 1259.635 kb on - strand, within ABZR86_RS05570 at 1259.635 kb on - strand, within ABZR86_RS05570 at 1259.635 kb on - strand, within ABZR86_RS05570 at 1259.635 kb on - strand, within ABZR86_RS05570 at 1259.950 kb on - strand, within ABZR86_RS05570 at 1260.100 kb on + strand at 1260.101 kb on - strand at 1260.105 kb on + strand at 1260.106 kb on - strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.135 kb on + strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.136 kb on - strand at 1260.139 kb on + strand at 1260.139 kb on + strand at 1260.139 kb on + strand at 1260.139 kb on + strand at 1260.140 kb on - strand at 1260.140 kb on - strand at 1260.140 kb on - strand at 1260.140 kb on - strand at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.274 kb on + strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.275 kb on - strand, within ABZR86_RS05575 at 1260.283 kb on + strand, within ABZR86_RS05575 at 1260.283 kb on + strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.284 kb on - strand, within ABZR86_RS05575 at 1260.355 kb on + strand, within ABZR86_RS05575 at 1260.356 kb on - strand, within ABZR86_RS05575 at 1260.356 kb on - strand, within ABZR86_RS05575 at 1260.356 kb on - strand, within ABZR86_RS05575 at 1260.356 kb on - strand, within ABZR86_RS05575 at 1260.409 kb on + strand, within ABZR86_RS05575 at 1260.409 kb on + strand, within ABZR86_RS05575 at 1260.409 kb on + strand, within ABZR86_RS05575 at 1260.409 kb on + strand, within ABZR86_RS05575 at 1260.410 kb on - strand, within ABZR86_RS05575 at 1260.479 kb on + strand at 1260.479 kb on + strand at 1260.479 kb on + strand at 1260.480 kb on - strand at 1260.480 kb on - strand at 1260.507 kb on + strand at 1260.507 kb on + strand at 1260.507 kb on + strand at 1260.508 kb on - strand at 1260.508 kb on - strand at 1260.508 kb on - strand at 1260.508 kb on - strand at 1260.508 kb on - strand at 1260.508 kb on - strand at 1260.515 kb on - strand at 1260.515 kb on - strand at 1260.515 kb on - strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.549 kb on + strand at 1260.550 kb on - strand at 1260.550 kb on - strand at 1260.550 kb on - strand at 1260.550 kb on - strand at 1260.550 kb on - strand at 1260.550 kb on - strand at 1260.550 kb on - strand at 1260.616 kb on + strand at 1260.616 kb on + strand at 1260.617 kb on - strand at 1260.617 kb on - strand at 1260.617 kb on - strand at 1260.617 kb on - strand at 1260.634 kb on + strand at 1260.634 kb on + strand at 1260.634 kb on + strand at 1260.634 kb on + strand at 1260.635 kb on - strand at 1260.835 kb on + strand, within ABZR86_RS05580 at 1260.836 kb on - strand, within ABZR86_RS05580 at 1260.974 kb on - strand, within ABZR86_RS05580 at 1261.006 kb on + strand, within ABZR86_RS05580 at 1261.006 kb on + strand, within ABZR86_RS05580 at 1261.006 kb on + strand, within ABZR86_RS05580 at 1261.007 kb on - strand, within ABZR86_RS05580 at 1261.007 kb on - strand, within ABZR86_RS05580 at 1261.007 kb on - strand, within ABZR86_RS05580
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,259,316 - -1.3 1,259,316 - +0.1 1,259,316 - +0.5 1,259,316 - -0.3 1,259,375 - -1.8 1,259,375 - +0.1 1,259,375 - -0.4 1,259,375 - -0.7 1,259,375 - -1.1 1,259,375 - +0.3 1,259,375 - -0.0 1,259,375 - +0.7 1,259,453 - ABZR86_RS05570 0.10 -0.8 1,259,516 - ABZR86_RS05570 0.19 +0.1 1,259,516 - ABZR86_RS05570 0.19 -1.3 1,259,587 - ABZR86_RS05570 0.30 -2.4 1,259,634 + ABZR86_RS05570 0.37 -1.1 1,259,634 + ABZR86_RS05570 0.37 -1.3 1,259,634 + ABZR86_RS05570 0.37 -1.6 1,259,635 - ABZR86_RS05570 0.37 -0.9 1,259,635 - ABZR86_RS05570 0.37 +3.1 1,259,635 - ABZR86_RS05570 0.37 -0.4 1,259,635 - ABZR86_RS05570 0.37 -1.6 1,259,950 - ABZR86_RS05570 0.82 +0.6 1,260,100 + -0.7 1,260,101 - -1.7 1,260,105 + -0.2 1,260,106 - +0.4 1,260,135 + -2.4 1,260,135 + -1.1 1,260,135 + -0.7 1,260,135 + +0.4 1,260,135 + +0.2 1,260,135 + +0.1 1,260,135 + -0.1 1,260,135 + +0.6 1,260,136 - +1.2 1,260,136 - -0.6 1,260,136 - +1.0 1,260,136 - +0.3 1,260,136 - +0.9 1,260,136 - +1.3 1,260,136 - +0.8 1,260,136 - +0.4 1,260,136 - -0.7 1,260,139 + -1.3 1,260,139 + +0.2 1,260,139 + +0.2 1,260,139 + +1.4 1,260,140 - -0.5 1,260,140 - -0.8 1,260,140 - +0.4 1,260,140 - +0.7 1,260,274 + ABZR86_RS05575 0.36 -0.5 1,260,274 + ABZR86_RS05575 0.36 -0.2 1,260,274 + ABZR86_RS05575 0.36 -0.2 1,260,274 + ABZR86_RS05575 0.36 +0.9 1,260,274 + ABZR86_RS05575 0.36 -0.9 1,260,274 + ABZR86_RS05575 0.36 +0.9 1,260,274 + ABZR86_RS05575 0.36 +0.8 1,260,274 + ABZR86_RS05575 0.36 +2.1 1,260,274 + ABZR86_RS05575 0.36 +0.2 1,260,274 + ABZR86_RS05575 0.36 -1.2 1,260,274 + ABZR86_RS05575 0.36 -0.2 1,260,274 + ABZR86_RS05575 0.36 +1.4 1,260,275 - ABZR86_RS05575 0.36 -1.3 1,260,275 - ABZR86_RS05575 0.36 +0.2 1,260,275 - ABZR86_RS05575 0.36 -1.1 1,260,275 - ABZR86_RS05575 0.36 +1.8 1,260,275 - ABZR86_RS05575 0.36 +2.5 1,260,275 - ABZR86_RS05575 0.36 +0.6 1,260,275 - ABZR86_RS05575 0.36 +0.5 1,260,275 - ABZR86_RS05575 0.36 +0.8 1,260,283 + ABZR86_RS05575 0.39 +0.0 1,260,283 + ABZR86_RS05575 0.39 +0.5 1,260,284 - ABZR86_RS05575 0.39 +0.2 1,260,284 - ABZR86_RS05575 0.39 +2.0 1,260,284 - ABZR86_RS05575 0.39 -0.6 1,260,284 - ABZR86_RS05575 0.39 +0.6 1,260,284 - ABZR86_RS05575 0.39 +0.4 1,260,284 - ABZR86_RS05575 0.39 +0.2 1,260,284 - ABZR86_RS05575 0.39 -0.4 1,260,284 - ABZR86_RS05575 0.39 -0.2 1,260,284 - ABZR86_RS05575 0.39 -1.4 1,260,355 + ABZR86_RS05575 0.67 -0.4 1,260,356 - ABZR86_RS05575 0.67 +0.7 1,260,356 - ABZR86_RS05575 0.67 +1.3 1,260,356 - ABZR86_RS05575 0.67 -1.1 1,260,356 - ABZR86_RS05575 0.67 +0.7 1,260,409 + ABZR86_RS05575 0.87 +0.4 1,260,409 + ABZR86_RS05575 0.87 +0.1 1,260,409 + ABZR86_RS05575 0.87 +0.2 1,260,409 + ABZR86_RS05575 0.87 -0.9 1,260,410 - ABZR86_RS05575 0.88 +0.1 1,260,479 + +0.3 1,260,479 + -0.5 1,260,479 + -1.1 1,260,480 - -0.7 1,260,480 - +0.8 1,260,507 + -2.4 1,260,507 + +1.5 1,260,507 + +0.9 1,260,508 - +1.6 1,260,508 - -0.4 1,260,508 - -0.7 1,260,508 - -0.1 1,260,508 - -0.3 1,260,508 - -2.0 1,260,515 - -0.2 1,260,515 - +0.6 1,260,515 - +0.6 1,260,549 + -0.4 1,260,549 + -0.7 1,260,549 + -1.0 1,260,549 + +0.1 1,260,549 + +0.1 1,260,549 + +1.2 1,260,549 + -0.3 1,260,549 + +0.7 1,260,549 + +0.9 1,260,550 - -0.4 1,260,550 - -0.7 1,260,550 - -0.2 1,260,550 - +1.2 1,260,550 - -0.1 1,260,550 - +0.2 1,260,550 - -0.1 1,260,616 + +0.3 1,260,616 + -2.3 1,260,617 - -1.7 1,260,617 - -0.2 1,260,617 - -0.9 1,260,617 - +0.0 1,260,634 + +0.8 1,260,634 + -1.2 1,260,634 + -0.1 1,260,634 + -2.7 1,260,635 - -0.1 1,260,835 + ABZR86_RS05580 0.16 +0.2 1,260,836 - ABZR86_RS05580 0.16 -0.5 1,260,974 - ABZR86_RS05580 0.23 +0.2 1,261,006 + ABZR86_RS05580 0.25 -0.4 1,261,006 + ABZR86_RS05580 0.25 -0.2 1,261,006 + ABZR86_RS05580 0.25 -1.1 1,261,007 - ABZR86_RS05580 0.25 +0.1 1,261,007 - ABZR86_RS05580 0.25 -1.1 1,261,007 - ABZR86_RS05580 0.25 -0.1
Or see this region's nucleotide sequence