Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05545 and ABZR86_RS05550 are separated by 7 nucleotides ABZR86_RS05550 and ABZR86_RS05555 overlap by 4 nucleotides ABZR86_RS05555 and ABZR86_RS05560 are separated by 180 nucleotides ABZR86_RS05560 and ABZR86_RS05565 are separated by 69 nucleotides
ABZR86_RS05545: ABZR86_RS05545 - glycosyltransferase family 4 protein, at 1,254,519 to 1,255,553
_RS05545
ABZR86_RS05550: ABZR86_RS05550 - lipopolysaccharide assembly protein LapB, at 1,255,561 to 1,256,733
_RS05550
ABZR86_RS05555: ABZR86_RS05555 - lipopolysaccharide assembly protein LapA domain-containing protein, at 1,256,730 to 1,256,990
_RS05555
ABZR86_RS05560: ABZR86_RS05560 - integration host factor subunit beta, at 1,257,171 to 1,257,482
_RS05560
ABZR86_RS05565: ABZR86_RS05565 - 30S ribosomal protein S1, at 1,257,552 to 1,259,225
_RS05565
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1254.565 kb on + strand at 1254.565 kb on + strand at 1254.565 kb on + strand at 1254.604 kb on + strand at 1254.604 kb on + strand at 1254.604 kb on + strand at 1254.604 kb on + strand at 1254.604 kb on + strand at 1254.605 kb on - strand at 1254.605 kb on - strand at 1254.694 kb on + strand, within ABZR86_RS05545 at 1254.694 kb on + strand, within ABZR86_RS05545 at 1254.694 kb on + strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.695 kb on - strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.701 kb on + strand, within ABZR86_RS05545 at 1254.702 kb on - strand, within ABZR86_RS05545 at 1254.702 kb on - strand, within ABZR86_RS05545 at 1254.702 kb on - strand, within ABZR86_RS05545 at 1254.702 kb on - strand, within ABZR86_RS05545 at 1254.745 kb on + strand, within ABZR86_RS05545 at 1254.745 kb on + strand, within ABZR86_RS05545 at 1254.745 kb on + strand, within ABZR86_RS05545 at 1254.822 kb on + strand, within ABZR86_RS05545 at 1254.964 kb on + strand, within ABZR86_RS05545 at 1254.965 kb on - strand, within ABZR86_RS05545 at 1254.965 kb on - strand, within ABZR86_RS05545 at 1255.221 kb on + strand, within ABZR86_RS05545 at 1255.222 kb on - strand, within ABZR86_RS05545 at 1255.222 kb on - strand, within ABZR86_RS05545 at 1255.468 kb on + strand at 1255.468 kb on + strand at 1255.469 kb on - strand at 1255.504 kb on - strand at 1255.504 kb on - strand at 1255.504 kb on - strand at 1255.667 kb on + strand at 1255.884 kb on + strand, within ABZR86_RS05550 at 1255.953 kb on - strand, within ABZR86_RS05550 at 1256.180 kb on + strand, within ABZR86_RS05550 at 1256.180 kb on + strand, within ABZR86_RS05550 at 1256.378 kb on + strand, within ABZR86_RS05550 at 1256.378 kb on + strand, within ABZR86_RS05550 at 1256.378 kb on + strand, within ABZR86_RS05550 at 1256.379 kb on - strand, within ABZR86_RS05550 at 1256.379 kb on - strand, within ABZR86_RS05550 at 1256.379 kb on - strand, within ABZR86_RS05550 at 1256.537 kb on + strand, within ABZR86_RS05550 at 1256.538 kb on - strand, within ABZR86_RS05550 at 1256.595 kb on - strand, within ABZR86_RS05550 at 1256.720 kb on + strand at 1256.721 kb on - strand at 1256.721 kb on - strand at 1256.721 kb on - strand at 1257.081 kb on + strand at 1257.082 kb on - strand at 1257.082 kb on - strand at 1257.152 kb on + strand at 1257.152 kb on + strand at 1257.152 kb on + strand at 1257.172 kb on + strand at 1257.173 kb on - strand at 1257.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,254,565 + -1.7 1,254,565 + -0.4 1,254,565 + -0.9 1,254,604 + +1.0 1,254,604 + -1.5 1,254,604 + -0.4 1,254,604 + -0.0 1,254,604 + +0.1 1,254,605 - +1.1 1,254,605 - -0.9 1,254,694 + ABZR86_RS05545 0.17 -2.5 1,254,694 + ABZR86_RS05545 0.17 +0.4 1,254,694 + ABZR86_RS05545 0.17 -2.3 1,254,695 - ABZR86_RS05545 0.17 -0.7 1,254,695 - ABZR86_RS05545 0.17 +0.2 1,254,695 - ABZR86_RS05545 0.17 +0.6 1,254,695 - ABZR86_RS05545 0.17 -0.5 1,254,695 - ABZR86_RS05545 0.17 -0.7 1,254,695 - ABZR86_RS05545 0.17 -0.3 1,254,695 - ABZR86_RS05545 0.17 +1.6 1,254,701 + ABZR86_RS05545 0.18 -1.3 1,254,701 + ABZR86_RS05545 0.18 -0.4 1,254,701 + ABZR86_RS05545 0.18 -0.9 1,254,701 + ABZR86_RS05545 0.18 -1.0 1,254,701 + ABZR86_RS05545 0.18 -0.3 1,254,701 + ABZR86_RS05545 0.18 -0.8 1,254,701 + ABZR86_RS05545 0.18 +0.2 1,254,702 - ABZR86_RS05545 0.18 -1.6 1,254,702 - ABZR86_RS05545 0.18 -0.1 1,254,702 - ABZR86_RS05545 0.18 -0.0 1,254,702 - ABZR86_RS05545 0.18 +0.4 1,254,745 + ABZR86_RS05545 0.22 -1.1 1,254,745 + ABZR86_RS05545 0.22 -1.5 1,254,745 + ABZR86_RS05545 0.22 +0.0 1,254,822 + ABZR86_RS05545 0.29 +0.3 1,254,964 + ABZR86_RS05545 0.43 +0.1 1,254,965 - ABZR86_RS05545 0.43 +2.2 1,254,965 - ABZR86_RS05545 0.43 -0.4 1,255,221 + ABZR86_RS05545 0.68 -1.7 1,255,222 - ABZR86_RS05545 0.68 +0.5 1,255,222 - ABZR86_RS05545 0.68 -1.5 1,255,468 + +0.1 1,255,468 + +0.1 1,255,469 - +0.5 1,255,504 - -0.0 1,255,504 - -0.2 1,255,504 - +0.1 1,255,667 + -1.3 1,255,884 + ABZR86_RS05550 0.28 -1.2 1,255,953 - ABZR86_RS05550 0.33 -0.9 1,256,180 + ABZR86_RS05550 0.53 +0.9 1,256,180 + ABZR86_RS05550 0.53 -0.0 1,256,378 + ABZR86_RS05550 0.70 -2.0 1,256,378 + ABZR86_RS05550 0.70 -0.6 1,256,378 + ABZR86_RS05550 0.70 -1.3 1,256,379 - ABZR86_RS05550 0.70 +1.0 1,256,379 - ABZR86_RS05550 0.70 +0.3 1,256,379 - ABZR86_RS05550 0.70 -0.9 1,256,537 + ABZR86_RS05550 0.83 +1.6 1,256,538 - ABZR86_RS05550 0.83 +0.8 1,256,595 - ABZR86_RS05550 0.88 +1.1 1,256,720 + -0.6 1,256,721 - -0.1 1,256,721 - +1.2 1,256,721 - +1.4 1,257,081 + +0.8 1,257,082 - +0.1 1,257,082 - +0.3 1,257,152 + +0.8 1,257,152 + +0.3 1,257,152 + -1.5 1,257,172 + -0.4 1,257,173 - -0.2 1,257,554 - -0.9
Or see this region's nucleotide sequence