Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05050 and ABZR86_RS05055 are separated by 10 nucleotides ABZR86_RS05055 and ABZR86_RS05060 are separated by 13 nucleotides ABZR86_RS05060 and ABZR86_RS05065 are separated by 49 nucleotides
ABZR86_RS05050: ABZR86_RS05050 - energy transducer TonB, at 1,139,943 to 1,140,605
_RS05050
ABZR86_RS05055: ABZR86_RS05055 - biopolymer transporter ExbD, at 1,140,616 to 1,141,026
_RS05055
ABZR86_RS05060: ABZR86_RS05060 - DUF2341 domain-containing protein, at 1,141,040 to 1,142,830
_RS05060
ABZR86_RS05065: ABZR86_RS05065 - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 1,142,880 to 1,144,460
_RS05065
Position (kb)
1141
1142
1143 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1140.187 kb on + strand, within ABZR86_RS05050 at 1140.188 kb on - strand, within ABZR86_RS05050 at 1140.707 kb on + strand, within ABZR86_RS05055 at 1140.708 kb on - strand, within ABZR86_RS05055 at 1140.959 kb on + strand, within ABZR86_RS05055 at 1140.959 kb on + strand, within ABZR86_RS05055 at 1140.959 kb on + strand, within ABZR86_RS05055 at 1140.960 kb on - strand, within ABZR86_RS05055 at 1140.960 kb on - strand, within ABZR86_RS05055 at 1141.018 kb on + strand at 1141.018 kb on + strand at 1141.153 kb on - strand at 1141.153 kb on - strand at 1141.153 kb on - strand at 1141.153 kb on - strand at 1141.158 kb on + strand at 1141.491 kb on + strand, within ABZR86_RS05060 at 1141.980 kb on + strand, within ABZR86_RS05060 at 1142.151 kb on + strand, within ABZR86_RS05060 at 1142.152 kb on - strand, within ABZR86_RS05060 at 1142.152 kb on - strand, within ABZR86_RS05060 at 1142.392 kb on - strand, within ABZR86_RS05060 at 1142.392 kb on - strand, within ABZR86_RS05060 at 1142.460 kb on + strand, within ABZR86_RS05060 at 1142.461 kb on - strand, within ABZR86_RS05060 at 1142.461 kb on - strand, within ABZR86_RS05060 at 1142.461 kb on - strand, within ABZR86_RS05060 at 1142.463 kb on + strand, within ABZR86_RS05060 at 1142.463 kb on + strand, within ABZR86_RS05060 at 1142.463 kb on + strand, within ABZR86_RS05060 at 1142.464 kb on - strand, within ABZR86_RS05060 at 1142.464 kb on - strand, within ABZR86_RS05060 at 1142.560 kb on - strand, within ABZR86_RS05060 at 1142.823 kb on + strand at 1142.824 kb on - strand at 1142.882 kb on - strand at 1142.959 kb on + strand at 1142.960 kb on - strand at 1143.112 kb on + strand, within ABZR86_RS05065 at 1143.319 kb on + strand, within ABZR86_RS05065 at 1143.320 kb on - strand, within ABZR86_RS05065 at 1143.320 kb on - strand, within ABZR86_RS05065 at 1143.472 kb on + strand, within ABZR86_RS05065 at 1143.493 kb on + strand, within ABZR86_RS05065 at 1143.493 kb on + strand, within ABZR86_RS05065 at 1143.494 kb on - strand, within ABZR86_RS05065 at 1143.494 kb on - strand, within ABZR86_RS05065 at 1143.494 kb on - strand, within ABZR86_RS05065 at 1143.494 kb on - strand, within ABZR86_RS05065 at 1143.688 kb on + strand, within ABZR86_RS05065 at 1143.734 kb on - strand, within ABZR86_RS05065 at 1143.745 kb on + strand, within ABZR86_RS05065 at 1143.745 kb on + strand, within ABZR86_RS05065 at 1143.745 kb on + strand, within ABZR86_RS05065 at 1143.745 kb on + strand, within ABZR86_RS05065 at 1143.745 kb on + strand, within ABZR86_RS05065 at 1143.746 kb on - strand, within ABZR86_RS05065 at 1143.746 kb on - strand, within ABZR86_RS05065 at 1143.746 kb on - strand, within ABZR86_RS05065 at 1143.746 kb on - strand, within ABZR86_RS05065 at 1143.784 kb on + strand, within ABZR86_RS05065 at 1143.784 kb on + strand, within ABZR86_RS05065 at 1143.784 kb on + strand, within ABZR86_RS05065 at 1143.784 kb on + strand, within ABZR86_RS05065 at 1143.785 kb on - strand, within ABZR86_RS05065 at 1143.785 kb on - strand, within ABZR86_RS05065 at 1143.785 kb on - strand, within ABZR86_RS05065 at 1143.785 kb on - strand, within ABZR86_RS05065 at 1143.785 kb on - strand, within ABZR86_RS05065
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,140,187 + ABZR86_RS05050 0.37 +0.4 1,140,188 - ABZR86_RS05050 0.37 +0.1 1,140,707 + ABZR86_RS05055 0.22 -0.2 1,140,708 - ABZR86_RS05055 0.22 +0.0 1,140,959 + ABZR86_RS05055 0.83 +0.2 1,140,959 + ABZR86_RS05055 0.83 +0.3 1,140,959 + ABZR86_RS05055 0.83 -0.9 1,140,960 - ABZR86_RS05055 0.84 +0.6 1,140,960 - ABZR86_RS05055 0.84 -0.4 1,141,018 + -0.7 1,141,018 + +2.6 1,141,153 - -0.2 1,141,153 - +1.0 1,141,153 - -0.2 1,141,153 - +0.8 1,141,158 + +0.2 1,141,491 + ABZR86_RS05060 0.25 -0.5 1,141,980 + ABZR86_RS05060 0.52 +0.0 1,142,151 + ABZR86_RS05060 0.62 +1.6 1,142,152 - ABZR86_RS05060 0.62 -0.5 1,142,152 - ABZR86_RS05060 0.62 +0.1 1,142,392 - ABZR86_RS05060 0.75 -0.2 1,142,392 - ABZR86_RS05060 0.75 +0.6 1,142,460 + ABZR86_RS05060 0.79 -0.0 1,142,461 - ABZR86_RS05060 0.79 +0.4 1,142,461 - ABZR86_RS05060 0.79 -0.5 1,142,461 - ABZR86_RS05060 0.79 -0.3 1,142,463 + ABZR86_RS05060 0.79 -0.1 1,142,463 + ABZR86_RS05060 0.79 +0.3 1,142,463 + ABZR86_RS05060 0.79 +0.1 1,142,464 - ABZR86_RS05060 0.80 +0.1 1,142,464 - ABZR86_RS05060 0.80 -0.8 1,142,560 - ABZR86_RS05060 0.85 +0.4 1,142,823 + -0.3 1,142,824 - -0.4 1,142,882 - -0.9 1,142,959 + +2.4 1,142,960 - +0.3 1,143,112 + ABZR86_RS05065 0.15 -0.1 1,143,319 + ABZR86_RS05065 0.28 +1.1 1,143,320 - ABZR86_RS05065 0.28 -0.0 1,143,320 - ABZR86_RS05065 0.28 +0.0 1,143,472 + ABZR86_RS05065 0.37 +0.1 1,143,493 + ABZR86_RS05065 0.39 +3.3 1,143,493 + ABZR86_RS05065 0.39 -0.6 1,143,494 - ABZR86_RS05065 0.39 -0.2 1,143,494 - ABZR86_RS05065 0.39 -1.4 1,143,494 - ABZR86_RS05065 0.39 -0.9 1,143,494 - ABZR86_RS05065 0.39 -1.1 1,143,688 + ABZR86_RS05065 0.51 -0.5 1,143,734 - ABZR86_RS05065 0.54 +0.1 1,143,745 + ABZR86_RS05065 0.55 -0.2 1,143,745 + ABZR86_RS05065 0.55 +0.3 1,143,745 + ABZR86_RS05065 0.55 -1.6 1,143,745 + ABZR86_RS05065 0.55 +0.3 1,143,745 + ABZR86_RS05065 0.55 +0.4 1,143,746 - ABZR86_RS05065 0.55 -0.1 1,143,746 - ABZR86_RS05065 0.55 +0.1 1,143,746 - ABZR86_RS05065 0.55 +0.7 1,143,746 - ABZR86_RS05065 0.55 -0.1 1,143,784 + ABZR86_RS05065 0.57 -1.5 1,143,784 + ABZR86_RS05065 0.57 +0.2 1,143,784 + ABZR86_RS05065 0.57 -1.8 1,143,784 + ABZR86_RS05065 0.57 -1.5 1,143,785 - ABZR86_RS05065 0.57 +0.0 1,143,785 - ABZR86_RS05065 0.57 -0.3 1,143,785 - ABZR86_RS05065 0.57 -0.5 1,143,785 - ABZR86_RS05065 0.57 +0.3 1,143,785 - ABZR86_RS05065 0.57 -0.1
Or see this region's nucleotide sequence