Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS04905 and ABZR86_RS04910 overlap by 1 nucleotides ABZR86_RS04910 and ABZR86_RS04915 are separated by 4 nucleotides ABZR86_RS04915 and ABZR86_RS04920 are separated by 26 nucleotides ABZR86_RS04920 and ABZR86_RS04925 overlap by 4 nucleotides
ABZR86_RS04905: ABZR86_RS04905 - hypothetical protein, at 1,098,995 to 1,099,468
_RS04905
ABZR86_RS04910: ABZR86_RS04910 - DUF4150 domain-containing protein, at 1,099,468 to 1,099,866
_RS04910
ABZR86_RS04915: ABZR86_RS04915 - DUF3540 domain-containing protein, at 1,099,871 to 1,100,410
_RS04915
ABZR86_RS04920: ABZR86_RS04920 - pentapeptide repeat-containing protein, at 1,100,437 to 1,101,519
_RS04920
ABZR86_RS04925: ABZR86_RS04925 - DUF2169 domain-containing protein, at 1,101,516 to 1,104,104
_RS04925
Position (kb) 1100
1101
1102 Strain fitness (log2 ratio) -1
0
1 at 1099.836 kb on + strand at 1099.837 kb on - strand at 1099.995 kb on + strand, within ABZR86_RS04915 at 1099.996 kb on - strand, within ABZR86_RS04915 at 1099.996 kb on - strand, within ABZR86_RS04915 at 1100.494 kb on - strand at 1101.170 kb on - strand, within ABZR86_RS04920 at 1101.658 kb on + strand at 1101.718 kb on + strand at 1101.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 1,099,836 + -0.5 1,099,837 - -0.5 1,099,995 + ABZR86_RS04915 0.23 +0.1 1,099,996 - ABZR86_RS04915 0.23 +0.7 1,099,996 - ABZR86_RS04915 0.23 -1.4 1,100,494 - -0.4 1,101,170 - ABZR86_RS04920 0.68 -1.0 1,101,658 + -0.1 1,101,718 + +0.9 1,101,719 - -0.9
Or see this region's nucleotide sequence