Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS02235 and ABZR86_RS02240 overlap by 4 nucleotides ABZR86_RS02240 and ABZR86_RS02245 are separated by 67 nucleotides ABZR86_RS02245 and ABZR86_RS02250 are separated by 71 nucleotides ABZR86_RS02250 and ABZR86_RS02255 are separated by 48 nucleotides ABZR86_RS02255 and ABZR86_RS02260 are separated by 157 nucleotides
ABZR86_RS02235: ABZR86_RS02235 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 480,904 to 481,683
_RS02235
ABZR86_RS02240: ABZR86_RS02240 - low molecular weight protein-tyrosine-phosphatase, at 481,680 to 482,147
_RS02240
ABZR86_RS02245: ABZR86_RS02245 - excinuclease ABC subunit UvrC, at 482,215 to 484,041
_RS02245
ABZR86_RS02250: ABZR86_RS02250 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 484,113 to 484,730
_RS02250
ABZR86_RS02255: ABZR86_RS02255 - tRNA-Gly, at 484,779 to 484,854
_RS02255
ABZR86_RS02260: ABZR86_RS02260 - tRNA-Gly, at 485,012 to 485,087
_RS02260
Position (kb) 482
483
484
485 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 481.718 kb on + strand at 481.718 kb on + strand at 481.719 kb on - strand at 481.923 kb on + strand, within ABZR86_RS02240 at 481.924 kb on - strand, within ABZR86_RS02240 at 482.059 kb on - strand, within ABZR86_RS02240 at 482.059 kb on - strand, within ABZR86_RS02240 at 482.062 kb on - strand, within ABZR86_RS02240 at 482.182 kb on - strand at 482.182 kb on - strand at 482.203 kb on - strand at 482.214 kb on + strand at 482.214 kb on + strand at 482.214 kb on + strand at 482.214 kb on + strand at 482.214 kb on + strand at 482.214 kb on + strand at 482.215 kb on - strand at 482.215 kb on - strand at 482.215 kb on - strand at 482.287 kb on + strand at 482.288 kb on - strand at 482.288 kb on - strand at 482.293 kb on + strand at 482.294 kb on - strand at 482.320 kb on + strand at 482.320 kb on + strand at 482.320 kb on + strand at 482.320 kb on + strand at 482.321 kb on - strand at 482.321 kb on - strand at 482.321 kb on - strand at 482.362 kb on + strand at 482.362 kb on + strand at 482.362 kb on + strand at 482.363 kb on - strand at 482.363 kb on - strand at 482.363 kb on - strand at 482.363 kb on - strand at 482.500 kb on + strand, within ABZR86_RS02245 at 482.500 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.536 kb on + strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.537 kb on - strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.542 kb on + strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.543 kb on - strand, within ABZR86_RS02245 at 482.553 kb on + strand, within ABZR86_RS02245 at 482.701 kb on + strand, within ABZR86_RS02245 at 482.701 kb on + strand, within ABZR86_RS02245 at 482.701 kb on + strand, within ABZR86_RS02245 at 482.702 kb on - strand, within ABZR86_RS02245 at 482.702 kb on - strand, within ABZR86_RS02245 at 482.702 kb on - strand, within ABZR86_RS02245 at 482.702 kb on - strand, within ABZR86_RS02245 at 483.133 kb on + strand, within ABZR86_RS02245 at 483.133 kb on + strand, within ABZR86_RS02245 at 483.133 kb on + strand, within ABZR86_RS02245 at 483.133 kb on + strand, within ABZR86_RS02245 at 483.133 kb on + strand, within ABZR86_RS02245 at 483.134 kb on - strand, within ABZR86_RS02245 at 483.134 kb on - strand, within ABZR86_RS02245 at 483.136 kb on + strand, within ABZR86_RS02245 at 483.136 kb on + strand, within ABZR86_RS02245 at 483.136 kb on + strand, within ABZR86_RS02245 at 483.136 kb on + strand, within ABZR86_RS02245 at 483.136 kb on + strand, within ABZR86_RS02245 at 483.137 kb on - strand, within ABZR86_RS02245 at 483.137 kb on - strand, within ABZR86_RS02245 at 483.137 kb on - strand, within ABZR86_RS02245 at 483.137 kb on - strand, within ABZR86_RS02245 at 483.137 kb on - strand, within ABZR86_RS02245 at 483.532 kb on + strand, within ABZR86_RS02245 at 483.533 kb on - strand, within ABZR86_RS02245 at 483.533 kb on - strand, within ABZR86_RS02245 at 483.575 kb on + strand, within ABZR86_RS02245 at 483.576 kb on - strand, within ABZR86_RS02245 at 483.576 kb on - strand, within ABZR86_RS02245 at 484.021 kb on + strand at 484.021 kb on + strand at 484.021 kb on + strand at 484.021 kb on + strand at 484.022 kb on - strand at 484.022 kb on - strand at 484.022 kb on - strand at 484.022 kb on - strand at 484.022 kb on - strand at 484.022 kb on - strand at 484.022 kb on - strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.112 kb on + strand at 484.727 kb on - strand at 484.760 kb on + strand at 484.760 kb on + strand at 484.760 kb on + strand at 484.786 kb on + strand at 484.786 kb on + strand at 484.786 kb on + strand at 484.798 kb on + strand, within ABZR86_RS02255 at 484.798 kb on + strand, within ABZR86_RS02255 at 484.798 kb on + strand, within ABZR86_RS02255 at 484.798 kb on + strand, within ABZR86_RS02255 at 484.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 481,718 + -0.4 481,718 + -0.8 481,719 - +0.0 481,923 + ABZR86_RS02240 0.52 +0.1 481,924 - ABZR86_RS02240 0.52 -0.5 482,059 - ABZR86_RS02240 0.81 -0.1 482,059 - ABZR86_RS02240 0.81 -0.6 482,062 - ABZR86_RS02240 0.82 -0.3 482,182 - -0.2 482,182 - -1.4 482,203 - -0.9 482,214 + +0.1 482,214 + -0.4 482,214 + +0.6 482,214 + -0.3 482,214 + -1.0 482,214 + -0.0 482,215 - -1.5 482,215 - -2.3 482,215 - -2.2 482,287 + -0.0 482,288 - -0.7 482,288 - -1.2 482,293 + -0.3 482,294 - +0.0 482,320 + +0.3 482,320 + -0.5 482,320 + -1.8 482,320 + +0.9 482,321 - -0.3 482,321 - -1.2 482,321 - -0.6 482,362 + -1.5 482,362 + +1.3 482,362 + -1.7 482,363 - -1.3 482,363 - -0.6 482,363 - -0.4 482,363 - -0.8 482,500 + ABZR86_RS02245 0.16 -2.3 482,500 + ABZR86_RS02245 0.16 -0.8 482,536 + ABZR86_RS02245 0.18 -0.2 482,536 + ABZR86_RS02245 0.18 -0.8 482,536 + ABZR86_RS02245 0.18 +0.7 482,536 + ABZR86_RS02245 0.18 -1.3 482,536 + ABZR86_RS02245 0.18 +0.6 482,536 + ABZR86_RS02245 0.18 -1.1 482,536 + ABZR86_RS02245 0.18 -1.6 482,537 - ABZR86_RS02245 0.18 +0.1 482,537 - ABZR86_RS02245 0.18 -1.5 482,537 - ABZR86_RS02245 0.18 -2.8 482,537 - ABZR86_RS02245 0.18 +0.2 482,537 - ABZR86_RS02245 0.18 -2.0 482,537 - ABZR86_RS02245 0.18 -1.0 482,537 - ABZR86_RS02245 0.18 -3.0 482,537 - ABZR86_RS02245 0.18 -0.2 482,537 - ABZR86_RS02245 0.18 -3.1 482,537 - ABZR86_RS02245 0.18 -1.1 482,537 - ABZR86_RS02245 0.18 -0.6 482,537 - ABZR86_RS02245 0.18 -1.3 482,537 - ABZR86_RS02245 0.18 +1.1 482,537 - ABZR86_RS02245 0.18 -1.7 482,542 + ABZR86_RS02245 0.18 -0.0 482,542 + ABZR86_RS02245 0.18 -0.3 482,542 + ABZR86_RS02245 0.18 -0.8 482,542 + ABZR86_RS02245 0.18 -1.3 482,542 + ABZR86_RS02245 0.18 -1.7 482,542 + ABZR86_RS02245 0.18 -0.5 482,542 + ABZR86_RS02245 0.18 -1.2 482,542 + ABZR86_RS02245 0.18 +0.2 482,542 + ABZR86_RS02245 0.18 +0.1 482,542 + ABZR86_RS02245 0.18 -0.2 482,542 + ABZR86_RS02245 0.18 -1.2 482,542 + ABZR86_RS02245 0.18 -0.9 482,543 - ABZR86_RS02245 0.18 -0.7 482,543 - ABZR86_RS02245 0.18 -0.3 482,543 - ABZR86_RS02245 0.18 -2.0 482,543 - ABZR86_RS02245 0.18 -1.0 482,543 - ABZR86_RS02245 0.18 -0.7 482,543 - ABZR86_RS02245 0.18 -0.6 482,543 - ABZR86_RS02245 0.18 -0.4 482,543 - ABZR86_RS02245 0.18 -1.6 482,553 + ABZR86_RS02245 0.19 -0.4 482,701 + ABZR86_RS02245 0.27 -0.7 482,701 + ABZR86_RS02245 0.27 -1.8 482,701 + ABZR86_RS02245 0.27 -1.2 482,702 - ABZR86_RS02245 0.27 -2.3 482,702 - ABZR86_RS02245 0.27 -1.4 482,702 - ABZR86_RS02245 0.27 +0.0 482,702 - ABZR86_RS02245 0.27 -1.0 483,133 + ABZR86_RS02245 0.50 +0.0 483,133 + ABZR86_RS02245 0.50 +0.4 483,133 + ABZR86_RS02245 0.50 -2.6 483,133 + ABZR86_RS02245 0.50 -0.5 483,133 + ABZR86_RS02245 0.50 -0.6 483,134 - ABZR86_RS02245 0.50 -1.0 483,134 - ABZR86_RS02245 0.50 -1.8 483,136 + ABZR86_RS02245 0.50 -0.2 483,136 + ABZR86_RS02245 0.50 -1.3 483,136 + ABZR86_RS02245 0.50 -0.2 483,136 + ABZR86_RS02245 0.50 +0.2 483,136 + ABZR86_RS02245 0.50 +0.5 483,137 - ABZR86_RS02245 0.50 -0.8 483,137 - ABZR86_RS02245 0.50 -2.5 483,137 - ABZR86_RS02245 0.50 -0.9 483,137 - ABZR86_RS02245 0.50 -3.1 483,137 - ABZR86_RS02245 0.50 +0.0 483,532 + ABZR86_RS02245 0.72 -0.6 483,533 - ABZR86_RS02245 0.72 -0.1 483,533 - ABZR86_RS02245 0.72 +0.1 483,575 + ABZR86_RS02245 0.74 -1.7 483,576 - ABZR86_RS02245 0.74 -1.3 483,576 - ABZR86_RS02245 0.74 -0.5 484,021 + -0.6 484,021 + -0.3 484,021 + -1.2 484,021 + -1.2 484,022 - -1.6 484,022 - -0.2 484,022 - -1.0 484,022 - +0.3 484,022 - -1.7 484,022 - -1.1 484,022 - -0.4 484,112 + -2.1 484,112 + -2.0 484,112 + -0.7 484,112 + +0.1 484,112 + -0.2 484,112 + -1.0 484,112 + -0.4 484,112 + -0.6 484,112 + -0.5 484,112 + -2.0 484,727 - +0.8 484,760 + +1.3 484,760 + +1.0 484,760 + +1.8 484,786 + +2.6 484,786 + +3.3 484,786 + +0.7 484,798 + ABZR86_RS02255 0.25 +3.7 484,798 + ABZR86_RS02255 0.25 +2.6 484,798 + ABZR86_RS02255 0.25 +2.3 484,798 + ABZR86_RS02255 0.25 +3.8 484,941 - +1.8
Or see this region's nucleotide sequence