Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS02185 and ABZR86_RS02190 overlap by 4 nucleotides ABZR86_RS02190 and ABZR86_RS02195 are separated by 138 nucleotides ABZR86_RS02195 and ABZR86_RS02200 are separated by 63 nucleotides
ABZR86_RS02185: ABZR86_RS02185 - tRNA 2-thiouridine(34) synthase MnmA, at 471,021 to 472,151
_RS02185
ABZR86_RS02190: ABZR86_RS02190 - NUDIX hydrolase, at 472,148 to 472,642
_RS02190
ABZR86_RS02195: ABZR86_RS02195 - ATP-dependent Clp protease adapter ClpS, at 472,781 to 473,107
_RS02195
ABZR86_RS02200: ABZR86_RS02200 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 473,171 to 475,441
_RS02200
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 471.785 kb on - strand, within ABZR86_RS02185 at 471.899 kb on - strand, within ABZR86_RS02185 at 472.406 kb on + strand, within ABZR86_RS02190 at 472.407 kb on - strand, within ABZR86_RS02190 at 472.407 kb on - strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.519 kb on - strand, within ABZR86_RS02190 at 472.519 kb on - strand, within ABZR86_RS02190 at 472.558 kb on - strand, within ABZR86_RS02190 at 472.558 kb on - strand, within ABZR86_RS02190 at 472.558 kb on - strand, within ABZR86_RS02190 at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.654 kb on - strand at 472.654 kb on - strand at 472.654 kb on - strand at 472.654 kb on - strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.710 kb on + strand at 472.710 kb on + strand at 472.710 kb on + strand at 472.710 kb on + strand at 472.711 kb on - strand at 472.711 kb on - strand at 472.748 kb on + strand at 472.749 kb on - strand at 472.749 kb on - strand at 472.749 kb on - strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.984 kb on + strand, within ABZR86_RS02195 at 472.984 kb on + strand, within ABZR86_RS02195 at 472.984 kb on + strand, within ABZR86_RS02195 at 472.984 kb on + strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 473.057 kb on + strand, within ABZR86_RS02195 at 473.058 kb on - strand, within ABZR86_RS02195 at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.086 kb on - strand at 473.086 kb on - strand at 473.086 kb on - strand at 473.086 kb on - strand at 473.092 kb on + strand at 473.210 kb on + strand at 473.210 kb on + strand at 473.210 kb on + strand at 473.210 kb on + strand at 473.210 kb on + strand at 473.210 kb on + strand at 473.211 kb on - strand at 473.211 kb on - strand at 473.211 kb on - strand at 473.211 kb on - strand at 473.444 kb on + strand, within ABZR86_RS02200 at 473.444 kb on + strand, within ABZR86_RS02200 at 473.445 kb on - strand, within ABZR86_RS02200 at 473.531 kb on + strand, within ABZR86_RS02200 at 473.531 kb on + strand, within ABZR86_RS02200 at 473.531 kb on + strand, within ABZR86_RS02200 at 473.531 kb on + strand, within ABZR86_RS02200 at 473.531 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.576 kb on + strand, within ABZR86_RS02200 at 473.577 kb on - strand, within ABZR86_RS02200 at 473.577 kb on - strand, within ABZR86_RS02200 at 473.577 kb on - strand, within ABZR86_RS02200 at 473.577 kb on - strand, within ABZR86_RS02200 at 473.577 kb on - strand, within ABZR86_RS02200 at 473.687 kb on + strand, within ABZR86_RS02200 at 473.807 kb on + strand, within ABZR86_RS02200 at 473.807 kb on + strand, within ABZR86_RS02200 at 473.807 kb on + strand, within ABZR86_RS02200 at 473.808 kb on - strand, within ABZR86_RS02200 at 473.808 kb on - strand, within ABZR86_RS02200
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 471,785 - ABZR86_RS02185 0.68 +1.2 471,899 - ABZR86_RS02185 0.78 +0.8 472,406 + ABZR86_RS02190 0.52 -2.4 472,407 - ABZR86_RS02190 0.52 +0.0 472,407 - ABZR86_RS02190 0.52 -0.5 472,518 + ABZR86_RS02190 0.75 -0.1 472,518 + ABZR86_RS02190 0.75 +0.4 472,518 + ABZR86_RS02190 0.75 -0.4 472,518 + ABZR86_RS02190 0.75 -0.1 472,519 - ABZR86_RS02190 0.75 +0.1 472,519 - ABZR86_RS02190 0.75 -0.6 472,558 - ABZR86_RS02190 0.83 +0.9 472,558 - ABZR86_RS02190 0.83 -1.1 472,558 - ABZR86_RS02190 0.83 +1.5 472,653 + -0.9 472,653 + -0.2 472,653 + -0.9 472,653 + +0.0 472,653 + -1.2 472,653 + +0.4 472,654 - -0.6 472,654 - -1.6 472,654 - -0.4 472,654 - -0.6 472,655 + -0.5 472,655 + -0.2 472,655 + +1.3 472,655 + +0.6 472,655 + +0.2 472,655 + -0.2 472,655 + -0.4 472,655 + -0.9 472,655 + -0.2 472,656 - -1.3 472,656 - -1.5 472,656 - -0.3 472,656 - -0.1 472,656 - -0.9 472,656 - -1.5 472,656 - +0.1 472,656 - +0.5 472,656 - +0.6 472,656 - -0.0 472,656 - -0.9 472,656 - -1.1 472,656 - +0.5 472,656 - +0.8 472,656 - +0.1 472,656 - -0.5 472,710 + +0.8 472,710 + +2.2 472,710 + -0.3 472,710 + +0.3 472,711 - -2.2 472,711 - -0.6 472,748 + +0.7 472,749 - -1.4 472,749 - -1.1 472,749 - +0.1 472,750 + +0.5 472,750 + +0.4 472,750 + +0.4 472,750 + -0.6 472,750 + +0.3 472,751 - -1.7 472,751 - -0.7 472,751 - +0.4 472,751 - -0.9 472,751 - +1.0 472,984 + ABZR86_RS02195 0.62 -2.7 472,984 + ABZR86_RS02195 0.62 -1.3 472,984 + ABZR86_RS02195 0.62 -0.9 472,984 + ABZR86_RS02195 0.62 -2.6 472,985 - ABZR86_RS02195 0.62 -0.1 472,985 - ABZR86_RS02195 0.62 -0.6 472,985 - ABZR86_RS02195 0.62 -0.0 472,985 - ABZR86_RS02195 0.62 -0.2 473,057 + ABZR86_RS02195 0.84 -0.7 473,058 - ABZR86_RS02195 0.85 -0.5 473,085 + -1.3 473,085 + -1.9 473,085 + -0.8 473,085 + -0.6 473,085 + -0.8 473,085 + -0.7 473,085 + -2.9 473,085 + -1.5 473,086 - +0.7 473,086 - -1.2 473,086 - -1.2 473,086 - -0.4 473,092 + -0.1 473,210 + +1.8 473,210 + +0.4 473,210 + -0.8 473,210 + +0.5 473,210 + +1.4 473,210 + +1.6 473,211 - +0.8 473,211 - +0.7 473,211 - -1.0 473,211 - +0.8 473,444 + ABZR86_RS02200 0.12 +1.0 473,444 + ABZR86_RS02200 0.12 +0.3 473,445 - ABZR86_RS02200 0.12 +0.4 473,531 + ABZR86_RS02200 0.16 -0.3 473,531 + ABZR86_RS02200 0.16 +0.1 473,531 + ABZR86_RS02200 0.16 +0.7 473,531 + ABZR86_RS02200 0.16 +1.1 473,531 + ABZR86_RS02200 0.16 -1.3 473,576 + ABZR86_RS02200 0.18 -0.5 473,576 + ABZR86_RS02200 0.18 +0.3 473,576 + ABZR86_RS02200 0.18 +0.8 473,576 + ABZR86_RS02200 0.18 +0.4 473,576 + ABZR86_RS02200 0.18 -1.9 473,576 + ABZR86_RS02200 0.18 -0.1 473,576 + ABZR86_RS02200 0.18 +0.3 473,577 - ABZR86_RS02200 0.18 -0.0 473,577 - ABZR86_RS02200 0.18 +0.5 473,577 - ABZR86_RS02200 0.18 +0.2 473,577 - ABZR86_RS02200 0.18 +0.4 473,577 - ABZR86_RS02200 0.18 -0.5 473,687 + ABZR86_RS02200 0.23 -0.2 473,807 + ABZR86_RS02200 0.28 +2.1 473,807 + ABZR86_RS02200 0.28 -0.3 473,807 + ABZR86_RS02200 0.28 -0.2 473,808 - ABZR86_RS02200 0.28 +0.8 473,808 - ABZR86_RS02200 0.28 +0.5
Or see this region's nucleotide sequence