Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00910 and ABZR86_RS00915 are separated by 244 nucleotides ABZR86_RS00915 and ABZR86_RS00920 are separated by 98 nucleotides ABZR86_RS00920 and ABZR86_RS00925 are separated by 24 nucleotides ABZR86_RS00925 and ABZR86_RS00930 are separated by 185 nucleotides
ABZR86_RS00910: ABZR86_RS00910 - excinuclease ABC subunit UvrA, at 178,367 to 181,339
_RS00910
ABZR86_RS00915: ABZR86_RS00915 - DUF2059 domain-containing protein, at 181,584 to 182,303
_RS00915
ABZR86_RS00920: ABZR86_RS00920 - 50S ribosomal protein L21, at 182,402 to 182,716
_RS00920
ABZR86_RS00925: ABZR86_RS00925 - 50S ribosomal protein L27, at 182,741 to 182,998
_RS00925
ABZR86_RS00930: ABZR86_RS00930 - Obg family GTPase CgtA, at 183,184 to 184,254
_RS00930
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 180.588 kb on + strand, within ABZR86_RS00910 at 181.005 kb on + strand, within ABZR86_RS00910 at 181.020 kb on + strand, within ABZR86_RS00910 at 181.020 kb on + strand, within ABZR86_RS00910 at 181.020 kb on + strand, within ABZR86_RS00910 at 181.021 kb on - strand, within ABZR86_RS00910 at 181.021 kb on - strand, within ABZR86_RS00910 at 181.021 kb on - strand, within ABZR86_RS00910 at 181.026 kb on + strand, within ABZR86_RS00910 at 181.027 kb on - strand, within ABZR86_RS00910 at 181.027 kb on - strand, within ABZR86_RS00910 at 181.027 kb on - strand, within ABZR86_RS00910 at 181.162 kb on - strand at 181.182 kb on + strand at 181.183 kb on - strand at 181.203 kb on + strand at 181.203 kb on + strand at 181.203 kb on + strand at 181.203 kb on + strand at 181.203 kb on + strand at 181.203 kb on + strand at 181.203 kb on + strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.204 kb on - strand at 181.382 kb on + strand at 181.383 kb on - strand at 181.383 kb on - strand at 181.384 kb on + strand at 181.384 kb on + strand at 181.405 kb on + strand at 181.472 kb on + strand at 181.489 kb on + strand at 181.489 kb on + strand at 181.489 kb on + strand at 181.489 kb on + strand at 181.490 kb on - strand at 181.490 kb on - strand at 181.493 kb on - strand at 181.493 kb on - strand at 181.518 kb on - strand at 181.630 kb on + strand at 181.630 kb on + strand at 181.630 kb on + strand at 181.630 kb on + strand at 181.630 kb on + strand at 181.631 kb on - strand at 181.631 kb on - strand at 181.631 kb on - strand at 181.734 kb on + strand, within ABZR86_RS00915 at 181.734 kb on + strand, within ABZR86_RS00915 at 181.735 kb on - strand, within ABZR86_RS00915 at 181.735 kb on - strand, within ABZR86_RS00915 at 181.747 kb on - strand, within ABZR86_RS00915 at 181.747 kb on - strand, within ABZR86_RS00915 at 181.747 kb on - strand, within ABZR86_RS00915 at 181.797 kb on + strand, within ABZR86_RS00915 at 181.797 kb on + strand, within ABZR86_RS00915 at 181.797 kb on + strand, within ABZR86_RS00915 at 181.798 kb on - strand, within ABZR86_RS00915 at 181.798 kb on - strand, within ABZR86_RS00915 at 181.798 kb on - strand, within ABZR86_RS00915 at 181.798 kb on - strand, within ABZR86_RS00915 at 181.798 kb on - strand, within ABZR86_RS00915 at 181.846 kb on - strand, within ABZR86_RS00915 at 182.301 kb on + strand at 183.060 kb on + strand at 183.060 kb on + strand at 183.060 kb on + strand at 183.060 kb on + strand at 183.061 kb on - strand at 183.074 kb on + strand at 183.081 kb on + strand at 183.081 kb on + strand at 183.081 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.085 kb on + strand at 183.086 kb on - strand at 183.086 kb on - strand at 183.086 kb on - strand at 183.086 kb on - strand at 183.086 kb on - strand at 183.086 kb on - strand at 183.112 kb on + strand at 183.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 180,588 + ABZR86_RS00910 0.75 +0.2 181,005 + ABZR86_RS00910 0.89 +0.7 181,020 + ABZR86_RS00910 0.89 -0.2 181,020 + ABZR86_RS00910 0.89 -1.4 181,020 + ABZR86_RS00910 0.89 -0.2 181,021 - ABZR86_RS00910 0.89 +0.5 181,021 - ABZR86_RS00910 0.89 +0.6 181,021 - ABZR86_RS00910 0.89 -0.1 181,026 + ABZR86_RS00910 0.89 -0.3 181,027 - ABZR86_RS00910 0.89 -2.8 181,027 - ABZR86_RS00910 0.89 +0.5 181,027 - ABZR86_RS00910 0.89 +0.5 181,162 - -0.7 181,182 + +1.0 181,183 - +1.7 181,203 + -2.3 181,203 + +0.1 181,203 + +0.1 181,203 + +0.0 181,203 + -0.2 181,203 + +1.3 181,203 + +1.6 181,204 - +0.3 181,204 - +0.3 181,204 - +0.6 181,204 - -1.0 181,204 - +0.5 181,204 - +0.0 181,204 - +0.8 181,204 - +0.0 181,204 - +0.6 181,382 + -0.1 181,383 - -1.2 181,383 - -0.7 181,384 + +0.6 181,384 + +4.9 181,405 + -0.9 181,472 + +1.1 181,489 + -0.2 181,489 + +0.1 181,489 + -0.5 181,489 + -0.7 181,490 - +0.1 181,490 - +0.4 181,493 - -0.2 181,493 - -0.0 181,518 - +0.7 181,630 + +0.4 181,630 + +0.4 181,630 + +0.6 181,630 + +0.9 181,630 + -0.6 181,631 - -0.2 181,631 - +0.0 181,631 - +1.1 181,734 + ABZR86_RS00915 0.21 +0.1 181,734 + ABZR86_RS00915 0.21 +0.0 181,735 - ABZR86_RS00915 0.21 +1.2 181,735 - ABZR86_RS00915 0.21 +0.3 181,747 - ABZR86_RS00915 0.23 +0.7 181,747 - ABZR86_RS00915 0.23 +0.4 181,747 - ABZR86_RS00915 0.23 +0.5 181,797 + ABZR86_RS00915 0.30 +0.1 181,797 + ABZR86_RS00915 0.30 +1.2 181,797 + ABZR86_RS00915 0.30 -0.1 181,798 - ABZR86_RS00915 0.30 +0.5 181,798 - ABZR86_RS00915 0.30 +0.3 181,798 - ABZR86_RS00915 0.30 -0.2 181,798 - ABZR86_RS00915 0.30 +0.9 181,798 - ABZR86_RS00915 0.30 +3.0 181,846 - ABZR86_RS00915 0.36 -0.3 182,301 + -1.0 183,060 + -0.6 183,060 + -1.4 183,060 + +0.2 183,060 + +2.5 183,061 - +8.4 183,074 + +0.4 183,081 + -0.4 183,081 + +2.1 183,081 + -2.3 183,085 + +0.2 183,085 + +0.1 183,085 + +1.0 183,085 + -0.6 183,085 + -0.8 183,085 + +0.9 183,085 + -1.0 183,085 + -0.2 183,085 + -1.2 183,085 + -1.5 183,085 + +0.5 183,086 - +9.7 183,086 - +8.5 183,086 - +9.0 183,086 - +8.5 183,086 - +9.5 183,086 - +8.8 183,112 + -0.1 183,112 + -0.1
Or see this region's nucleotide sequence