Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00660 and ABZR86_RS00665 are separated by 83 nucleotides ABZR86_RS00665 and ABZR86_RS00670 are separated by 20 nucleotides ABZR86_RS00670 and ABZR86_RS00675 are separated by 15 nucleotides ABZR86_RS00675 and ABZR86_RS00680 are separated by 64 nucleotides
ABZR86_RS00660: ABZR86_RS00660 - recombinase RecA, at 129,643 to 130,677
_RS00660
ABZR86_RS00665: ABZR86_RS00665 - sulfur transferase domain-containing protein, at 130,761 to 131,312
_RS00665
ABZR86_RS00670: ABZR86_RS00670 - hypothetical protein, at 131,333 to 131,488
_RS00670
ABZR86_RS00675: ABZR86_RS00675 - regulatory protein RecX, at 131,504 to 132,025
_RS00675
ABZR86_RS00680: ABZR86_RS00680 - alanine--tRNA ligase, at 132,090 to 134,726
_RS00680
Position (kb) 131
132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 130.511 kb on - strand, within ABZR86_RS00660 at 130.516 kb on + strand, within ABZR86_RS00660 at 130.516 kb on + strand, within ABZR86_RS00660 at 130.516 kb on + strand, within ABZR86_RS00660 at 130.516 kb on + strand, within ABZR86_RS00660 at 130.517 kb on - strand, within ABZR86_RS00660 at 130.695 kb on + strand at 130.695 kb on + strand at 130.695 kb on + strand at 130.695 kb on + strand at 130.695 kb on + strand at 130.695 kb on + strand at 130.696 kb on - strand at 130.696 kb on - strand at 130.696 kb on - strand at 130.696 kb on - strand at 130.696 kb on - strand at 130.715 kb on + strand at 130.715 kb on + strand at 130.716 kb on - strand at 130.716 kb on - strand at 130.746 kb on + strand at 130.747 kb on - strand at 130.747 kb on - strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.767 kb on - strand at 130.767 kb on - strand at 130.767 kb on - strand at 130.767 kb on - strand at 130.771 kb on + strand at 130.771 kb on + strand at 130.771 kb on + strand at 130.771 kb on + strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.772 kb on - strand at 130.784 kb on + strand at 131.049 kb on + strand, within ABZR86_RS00665 at 131.049 kb on + strand, within ABZR86_RS00665 at 131.049 kb on + strand, within ABZR86_RS00665 at 131.049 kb on + strand, within ABZR86_RS00665 at 131.049 kb on + strand, within ABZR86_RS00665 at 131.049 kb on + strand, within ABZR86_RS00665 at 131.049 kb on + strand, within ABZR86_RS00665 at 131.050 kb on - strand, within ABZR86_RS00665 at 131.050 kb on - strand, within ABZR86_RS00665 at 131.050 kb on - strand, within ABZR86_RS00665 at 131.050 kb on - strand, within ABZR86_RS00665 at 131.050 kb on - strand, within ABZR86_RS00665 at 131.139 kb on + strand, within ABZR86_RS00665 at 131.140 kb on - strand, within ABZR86_RS00665 at 131.140 kb on - strand, within ABZR86_RS00665 at 131.140 kb on - strand, within ABZR86_RS00665 at 131.140 kb on - strand, within ABZR86_RS00665 at 131.358 kb on + strand, within ABZR86_RS00670 at 131.497 kb on - strand at 131.509 kb on + strand at 131.510 kb on - strand at 131.510 kb on - strand at 131.654 kb on + strand, within ABZR86_RS00675 at 131.706 kb on - strand, within ABZR86_RS00675 at 131.763 kb on - strand, within ABZR86_RS00675 at 131.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 130,511 - ABZR86_RS00660 0.84 -3.3 130,516 + ABZR86_RS00660 0.84 -1.0 130,516 + ABZR86_RS00660 0.84 -0.4 130,516 + ABZR86_RS00660 0.84 -2.3 130,516 + ABZR86_RS00660 0.84 -2.0 130,517 - ABZR86_RS00660 0.84 -1.8 130,695 + +0.5 130,695 + -0.5 130,695 + -0.1 130,695 + +0.7 130,695 + +0.5 130,695 + -0.4 130,696 - +0.3 130,696 - -0.6 130,696 - -0.5 130,696 - +0.2 130,696 - -0.7 130,715 + +0.4 130,715 + +1.5 130,716 - +0.2 130,716 - -0.7 130,746 + +0.2 130,747 - -0.4 130,747 - -1.0 130,766 + -1.2 130,766 + +0.2 130,766 + -0.5 130,766 + -0.8 130,766 + +0.2 130,766 + -1.3 130,766 + -0.3 130,767 - -1.3 130,767 - -0.2 130,767 - -1.3 130,767 - +0.5 130,771 + +0.2 130,771 + +0.4 130,771 + -0.8 130,771 + +0.9 130,772 - -0.9 130,772 - +1.3 130,772 - -1.2 130,772 - +0.4 130,772 - +0.3 130,772 - -0.3 130,772 - -0.1 130,772 - +0.6 130,784 + +0.4 131,049 + ABZR86_RS00665 0.52 -0.6 131,049 + ABZR86_RS00665 0.52 -0.1 131,049 + ABZR86_RS00665 0.52 +1.8 131,049 + ABZR86_RS00665 0.52 +1.2 131,049 + ABZR86_RS00665 0.52 -0.1 131,049 + ABZR86_RS00665 0.52 -1.4 131,049 + ABZR86_RS00665 0.52 -0.7 131,050 - ABZR86_RS00665 0.52 +0.7 131,050 - ABZR86_RS00665 0.52 -0.2 131,050 - ABZR86_RS00665 0.52 -0.0 131,050 - ABZR86_RS00665 0.52 +0.1 131,050 - ABZR86_RS00665 0.52 -1.7 131,139 + ABZR86_RS00665 0.68 -1.2 131,140 - ABZR86_RS00665 0.69 -0.4 131,140 - ABZR86_RS00665 0.69 +1.1 131,140 - ABZR86_RS00665 0.69 -0.7 131,140 - ABZR86_RS00665 0.69 -0.6 131,358 + ABZR86_RS00670 0.16 +0.0 131,497 - -0.2 131,509 + -1.4 131,510 - +0.0 131,510 - -1.9 131,654 + ABZR86_RS00675 0.29 -2.7 131,706 - ABZR86_RS00675 0.39 -2.6 131,763 - ABZR86_RS00675 0.50 -1.2 131,973 + -1.4
Or see this region's nucleotide sequence