Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00415 and ABZR86_RS00420 are separated by 76 nucleotides ABZR86_RS00420 and ABZR86_RS00425 overlap by 4 nucleotides ABZR86_RS00425 and ABZR86_RS00430 overlap by 4 nucleotides
ABZR86_RS00415: ABZR86_RS00415 - NAD(P)/FAD-dependent oxidoreductase, at 79,562 to 80,740
_RS00415
ABZR86_RS00420: ABZR86_RS00420 - NAD(P)-dependent oxidoreductase, at 80,817 to 81,695
_RS00420
ABZR86_RS00425: ABZR86_RS00425 - YaiI/YqxD family protein, at 81,692 to 82,186
_RS00425
ABZR86_RS00430: ABZR86_RS00430 - PhzF family phenazine biosynthesis protein, at 82,183 to 83,082
_RS00430
Position (kb) 80
81
82 Strain fitness (log2 ratio) -2
-1
0
1
2 at 80.104 kb on + strand, within ABZR86_RS00415 at 80.105 kb on - strand, within ABZR86_RS00415 at 80.203 kb on - strand, within ABZR86_RS00415 at 80.203 kb on - strand, within ABZR86_RS00415 at 80.294 kb on + strand, within ABZR86_RS00415 at 80.518 kb on - strand, within ABZR86_RS00415 at 80.642 kb on + strand at 80.642 kb on + strand at 80.642 kb on + strand at 80.642 kb on + strand at 80.642 kb on + strand at 80.643 kb on - strand at 80.643 kb on - strand at 80.643 kb on - strand at 80.643 kb on - strand at 80.643 kb on - strand at 80.687 kb on + strand at 80.687 kb on + strand at 80.688 kb on - strand at 80.793 kb on - strand at 80.812 kb on + strand at 80.812 kb on + strand at 80.813 kb on - strand at 81.153 kb on + strand, within ABZR86_RS00420 at 81.154 kb on - strand, within ABZR86_RS00420 at 81.209 kb on + strand, within ABZR86_RS00420 at 81.210 kb on - strand, within ABZR86_RS00420 at 81.525 kb on + strand, within ABZR86_RS00420 at 81.526 kb on - strand, within ABZR86_RS00420 at 81.694 kb on - strand at 81.694 kb on - strand at 81.694 kb on - strand at 81.700 kb on + strand at 81.700 kb on + strand at 81.701 kb on - strand at 81.701 kb on - strand at 81.701 kb on - strand at 81.701 kb on - strand at 81.701 kb on - strand at 82.113 kb on + strand, within ABZR86_RS00425 at 82.146 kb on - strand at 82.314 kb on + strand, within ABZR86_RS00430 at 82.314 kb on + strand, within ABZR86_RS00430 at 82.315 kb on - strand, within ABZR86_RS00430 at 82.315 kb on - strand, within ABZR86_RS00430 at 82.315 kb on - strand, within ABZR86_RS00430 at 82.316 kb on + strand, within ABZR86_RS00430 at 82.356 kb on + strand, within ABZR86_RS00430 at 82.357 kb on - strand, within ABZR86_RS00430 at 82.358 kb on + strand, within ABZR86_RS00430 at 82.359 kb on - strand, within ABZR86_RS00430 at 82.359 kb on - strand, within ABZR86_RS00430 at 82.359 kb on - strand, within ABZR86_RS00430 at 82.445 kb on + strand, within ABZR86_RS00430 at 82.613 kb on + strand, within ABZR86_RS00430 at 82.613 kb on + strand, within ABZR86_RS00430 at 82.614 kb on - strand, within ABZR86_RS00430 at 82.633 kb on + strand, within ABZR86_RS00430
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 80,104 + ABZR86_RS00415 0.46 +1.1 80,105 - ABZR86_RS00415 0.46 -0.7 80,203 - ABZR86_RS00415 0.54 +0.2 80,203 - ABZR86_RS00415 0.54 +0.1 80,294 + ABZR86_RS00415 0.62 -0.1 80,518 - ABZR86_RS00415 0.81 -0.6 80,642 + +0.8 80,642 + +0.5 80,642 + +0.8 80,642 + -1.1 80,642 + -0.2 80,643 - +0.5 80,643 - -0.4 80,643 - +0.7 80,643 - +0.3 80,643 - +1.8 80,687 + -0.5 80,687 + -0.2 80,688 - -0.1 80,793 - -1.1 80,812 + -0.2 80,812 + -1.2 80,813 - +0.4 81,153 + ABZR86_RS00420 0.38 -1.0 81,154 - ABZR86_RS00420 0.38 -0.7 81,209 + ABZR86_RS00420 0.45 -0.5 81,210 - ABZR86_RS00420 0.45 -0.3 81,525 + ABZR86_RS00420 0.81 -0.9 81,526 - ABZR86_RS00420 0.81 -0.5 81,694 - +0.8 81,694 - +0.5 81,694 - -1.6 81,700 + -0.4 81,700 + +0.4 81,701 - -1.7 81,701 - -0.9 81,701 - -0.6 81,701 - -0.2 81,701 - -0.4 82,113 + ABZR86_RS00425 0.85 -0.1 82,146 - +0.8 82,314 + ABZR86_RS00430 0.15 -0.6 82,314 + ABZR86_RS00430 0.15 -0.8 82,315 - ABZR86_RS00430 0.15 -0.2 82,315 - ABZR86_RS00430 0.15 -1.3 82,315 - ABZR86_RS00430 0.15 +0.6 82,316 + ABZR86_RS00430 0.15 -0.8 82,356 + ABZR86_RS00430 0.19 -0.0 82,357 - ABZR86_RS00430 0.19 -0.0 82,358 + ABZR86_RS00430 0.19 -1.3 82,359 - ABZR86_RS00430 0.20 +0.5 82,359 - ABZR86_RS00430 0.20 -1.3 82,359 - ABZR86_RS00430 0.20 -0.1 82,445 + ABZR86_RS00430 0.29 -0.2 82,613 + ABZR86_RS00430 0.48 +0.3 82,613 + ABZR86_RS00430 0.48 +0.4 82,614 - ABZR86_RS00430 0.48 +0.3 82,633 + ABZR86_RS00430 0.50 -1.8
Or see this region's nucleotide sequence