Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00065 and ABZR86_RS00070 are separated by 94 nucleotides ABZR86_RS00070 and ABZR86_RS00075 are separated by 99 nucleotides ABZR86_RS00075 and ABZR86_RS00080 are separated by 111 nucleotides ABZR86_RS00080 and ABZR86_RS00085 are separated by 100 nucleotides ABZR86_RS00085 and ABZR86_RS00090 are separated by 55 nucleotides
ABZR86_RS00065: ABZR86_RS00065 - hypothetical protein, at 13,680 to 14,177
_RS00065
ABZR86_RS00070: ABZR86_RS00070 - hypothetical protein, at 14,272 to 14,730
_RS00070
ABZR86_RS00075: ABZR86_RS00075 - glucose 1-dehydrogenase, at 14,830 to 15,576
_RS00075
ABZR86_RS00080: ABZR86_RS00080 - helix-turn-helix domain-containing protein, at 15,688 to 16,065
_RS00080
ABZR86_RS00085: ABZR86_RS00085 - hypothetical protein, at 16,166 to 16,315
_RS00085
ABZR86_RS00090: ABZR86_RS00090 - AraC family transcriptional regulator, at 16,371 to 17,195
_RS00090
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-1
0
1 at 13.838 kb on - strand, within ABZR86_RS00065 at 14.161 kb on - strand at 14.208 kb on - strand at 14.245 kb on - strand at 14.245 kb on - strand at 14.271 kb on + strand at 14.271 kb on + strand at 14.272 kb on - strand at 14.272 kb on - strand at 14.272 kb on - strand at 14.272 kb on - strand at 14.272 kb on - strand at 14.325 kb on - strand, within ABZR86_RS00070 at 14.333 kb on - strand, within ABZR86_RS00070 at 14.545 kb on + strand, within ABZR86_RS00070 at 14.546 kb on - strand, within ABZR86_RS00070 at 14.611 kb on - strand, within ABZR86_RS00070 at 14.651 kb on + strand, within ABZR86_RS00070 at 14.820 kb on - strand at 14.820 kb on - strand at 14.853 kb on - strand at 14.853 kb on - strand at 15.116 kb on + strand, within ABZR86_RS00075 at 15.467 kb on + strand, within ABZR86_RS00075 at 15.468 kb on - strand, within ABZR86_RS00075 at 15.468 kb on - strand, within ABZR86_RS00075 at 15.675 kb on + strand at 15.675 kb on + strand at 15.675 kb on + strand at 15.675 kb on + strand at 15.676 kb on - strand at 15.676 kb on - strand at 15.676 kb on - strand at 15.698 kb on + strand at 15.699 kb on - strand at 15.775 kb on + strand, within ABZR86_RS00080 at 15.775 kb on + strand, within ABZR86_RS00080 at 15.775 kb on + strand, within ABZR86_RS00080 at 15.776 kb on - strand, within ABZR86_RS00080 at 15.856 kb on - strand, within ABZR86_RS00080 at 15.856 kb on - strand, within ABZR86_RS00080 at 15.856 kb on - strand, within ABZR86_RS00080 at 15.997 kb on + strand, within ABZR86_RS00080 at 15.998 kb on - strand, within ABZR86_RS00080 at 15.998 kb on - strand, within ABZR86_RS00080 at 16.224 kb on + strand, within ABZR86_RS00085 at 16.224 kb on + strand, within ABZR86_RS00085 at 16.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 13,838 - ABZR86_RS00065 0.32 -1.2 14,161 - -0.8 14,208 - -0.5 14,245 - -1.0 14,245 - -1.4 14,271 + +0.2 14,271 + +1.0 14,272 - -0.5 14,272 - +0.5 14,272 - +0.3 14,272 - +0.2 14,272 - -0.4 14,325 - ABZR86_RS00070 0.12 +0.6 14,333 - ABZR86_RS00070 0.13 +0.2 14,545 + ABZR86_RS00070 0.59 -1.1 14,546 - ABZR86_RS00070 0.60 +1.0 14,611 - ABZR86_RS00070 0.74 -0.3 14,651 + ABZR86_RS00070 0.83 +0.2 14,820 - -1.0 14,820 - +0.1 14,853 - +0.6 14,853 - +0.0 15,116 + ABZR86_RS00075 0.38 +0.2 15,467 + ABZR86_RS00075 0.85 -0.6 15,468 - ABZR86_RS00075 0.85 -0.9 15,468 - ABZR86_RS00075 0.85 -0.9 15,675 + +0.0 15,675 + +0.3 15,675 + -0.3 15,675 + +0.2 15,676 - -0.4 15,676 - -0.3 15,676 - +0.1 15,698 + -0.4 15,699 - -0.1 15,775 + ABZR86_RS00080 0.23 +0.0 15,775 + ABZR86_RS00080 0.23 +0.3 15,775 + ABZR86_RS00080 0.23 -0.6 15,776 - ABZR86_RS00080 0.23 +1.2 15,856 - ABZR86_RS00080 0.44 -0.8 15,856 - ABZR86_RS00080 0.44 +0.7 15,856 - ABZR86_RS00080 0.44 -0.3 15,997 + ABZR86_RS00080 0.82 +0.2 15,998 - ABZR86_RS00080 0.82 -0.0 15,998 - ABZR86_RS00080 0.82 +1.0 16,224 + ABZR86_RS00085 0.39 +1.0 16,224 + ABZR86_RS00085 0.39 +0.4 16,409 - +0.3
Or see this region's nucleotide sequence