Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2253 and SMa2255 are separated by 2 nucleotides SMa2255 and SMa2257 overlap by 4 nucleotides SMa2257 and SMa2259 are separated by 6 nucleotides
SMa2253: SMa2253 - hypothetical protein, at 1,263,295 to 1,263,672
SMa2253
SMa2255: SMa2255 - hypothetical protein, at 1,263,675 to 1,264,013
SMa2255
SMa2257: SMa2257 - integrase-like protein, at 1,264,010 to 1,265,167
SMa2257
SMa2259: SMa2259 - hypothetical protein, at 1,265,174 to 1,265,599
SMa2259
Position (kb)
1264
1265
1266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1263.584 kb on + strand, within SMa2253 at 1263.585 kb on - strand, within SMa2253 at 1263.689 kb on - strand at 1263.689 kb on - strand at 1263.694 kb on + strand at 1263.694 kb on + strand at 1263.694 kb on + strand at 1263.695 kb on - strand at 1263.695 kb on - strand at 1263.762 kb on + strand, within SMa2255 at 1263.763 kb on - strand, within SMa2255 at 1263.785 kb on + strand, within SMa2255 at 1263.787 kb on + strand, within SMa2255 at 1263.788 kb on - strand, within SMa2255 at 1263.788 kb on - strand, within SMa2255 at 1263.788 kb on - strand, within SMa2255 at 1263.788 kb on - strand, within SMa2255 at 1263.961 kb on - strand, within SMa2255 at 1263.963 kb on - strand, within SMa2255 at 1263.963 kb on - strand, within SMa2255 at 1263.963 kb on - strand, within SMa2255 at 1263.963 kb on - strand, within SMa2255 at 1263.963 kb on - strand, within SMa2255 at 1263.987 kb on + strand at 1263.987 kb on + strand at 1263.987 kb on + strand at 1264.171 kb on + strand, within SMa2257 at 1264.171 kb on + strand, within SMa2257 at 1264.171 kb on + strand, within SMa2257 at 1264.171 kb on + strand, within SMa2257 at 1264.171 kb on + strand, within SMa2257 at 1264.172 kb on - strand, within SMa2257 at 1264.600 kb on + strand, within SMa2257 at 1264.601 kb on - strand, within SMa2257 at 1264.636 kb on - strand, within SMa2257 at 1264.636 kb on - strand, within SMa2257 at 1264.636 kb on - strand, within SMa2257 at 1265.027 kb on + strand, within SMa2257 at 1265.114 kb on + strand at 1265.118 kb on - strand at 1265.172 kb on - strand at 1265.172 kb on - strand at 1265.248 kb on + strand, within SMa2259 at 1265.249 kb on - strand, within SMa2259 at 1265.380 kb on - strand, within SMa2259 at 1265.380 kb on - strand, within SMa2259 at 1265.380 kb on - strand, within SMa2259 at 1265.380 kb on - strand, within SMa2259 at 1265.525 kb on + strand, within SMa2259 at 1265.526 kb on - strand, within SMa2259 at 1265.578 kb on - strand at 1265.634 kb on + strand at 1265.634 kb on + strand at 1265.635 kb on - strand at 1265.635 kb on - strand at 1265.819 kb on + strand at 1265.931 kb on - strand at 1266.051 kb on + strand at 1266.095 kb on - strand at 1266.095 kb on - strand at 1266.117 kb on + strand at 1266.117 kb on + strand at 1266.118 kb on - strand at 1266.147 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 1,263,584 + SMa2253 0.76 +1.3 1,263,585 - SMa2253 0.77 -0.3 1,263,689 - -2.0 1,263,689 - -1.9 1,263,694 + -0.1 1,263,694 + +0.7 1,263,694 + -1.1 1,263,695 - -0.0 1,263,695 - -1.2 1,263,762 + SMa2255 0.26 +0.6 1,263,763 - SMa2255 0.26 +0.7 1,263,785 + SMa2255 0.32 -0.4 1,263,787 + SMa2255 0.33 -0.6 1,263,788 - SMa2255 0.33 +0.4 1,263,788 - SMa2255 0.33 -0.1 1,263,788 - SMa2255 0.33 -1.3 1,263,788 - SMa2255 0.33 -0.1 1,263,961 - SMa2255 0.84 -3.3 1,263,963 - SMa2255 0.85 +0.5 1,263,963 - SMa2255 0.85 -0.0 1,263,963 - SMa2255 0.85 +0.1 1,263,963 - SMa2255 0.85 +0.8 1,263,963 - SMa2255 0.85 -2.8 1,263,987 + -0.0 1,263,987 + -1.0 1,263,987 + -0.4 1,264,171 + SMa2257 0.14 +2.0 1,264,171 + SMa2257 0.14 -1.9 1,264,171 + SMa2257 0.14 -0.8 1,264,171 + SMa2257 0.14 -1.2 1,264,171 + SMa2257 0.14 +1.1 1,264,172 - SMa2257 0.14 +0.2 1,264,600 + SMa2257 0.51 -2.2 1,264,601 - SMa2257 0.51 -0.2 1,264,636 - SMa2257 0.54 -0.7 1,264,636 - SMa2257 0.54 -1.0 1,264,636 - SMa2257 0.54 -0.4 1,265,027 + SMa2257 0.88 +0.1 1,265,114 + +0.4 1,265,118 - -0.2 1,265,172 - +0.6 1,265,172 - -1.7 1,265,248 + SMa2259 0.17 +0.6 1,265,249 - SMa2259 0.18 +0.7 1,265,380 - SMa2259 0.48 -0.8 1,265,380 - SMa2259 0.48 -0.9 1,265,380 - SMa2259 0.48 -0.8 1,265,380 - SMa2259 0.48 -0.2 1,265,525 + SMa2259 0.82 +0.2 1,265,526 - SMa2259 0.83 -1.7 1,265,578 - +0.3 1,265,634 + +0.4 1,265,634 + -0.4 1,265,635 - +1.3 1,265,635 - +0.4 1,265,819 + -0.7 1,265,931 - +1.1 1,266,051 + -0.6 1,266,095 - +0.3 1,266,095 - -1.9 1,266,117 + +0.0 1,266,117 + -0.7 1,266,118 - +0.7 1,266,147 + -0.4
Or see this region's nucleotide sequence