Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1483 and SMa5009 are separated by 60 nucleotides SMa5009 and SMa1485 overlap by 4 nucleotides SMa1485 and SMa1487 are separated by 228 nucleotides SMa1487 and SMa1488 overlap by 4 nucleotides
SMa1483: SMa1483 - dehydrogenase, at 815,138 to 817,558
SMa1483
SMa5009: SMa5009 - hypothetical protein, at 817,619 to 817,969
SMa5009
SMa1485: SMa1485 - Permease, MFS, at 817,966 to 819,255
SMa1485
SMa1487: SMa1487 - cytochrome C, at 819,484 to 819,852
SMa1487
SMa1488: SMa1488 - oxidoreductase, at 819,849 to 822,092
SMa1488
Position (kb) 817
818
819
820 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 817.187 kb on + strand, within SMa1483 at 817.188 kb on - strand, within SMa1483 at 817.404 kb on - strand at 817.406 kb on - strand at 817.597 kb on - strand at 817.731 kb on + strand at 817.731 kb on + strand, within SMa5009 at 817.732 kb on - strand, within SMa5009 at 817.761 kb on + strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.765 kb on - strand, within SMa5009 at 817.834 kb on - strand, within SMa5009 at 817.858 kb on - strand, within SMa5009 at 817.858 kb on - strand, within SMa5009 at 817.973 kb on - strand at 818.420 kb on + strand, within SMa1485 at 818.420 kb on + strand, within SMa1485 at 818.753 kb on + strand, within SMa1485 at 818.754 kb on - strand, within SMa1485 at 818.754 kb on - strand, within SMa1485 at 818.940 kb on - strand, within SMa1485 at 819.108 kb on + strand, within SMa1485 at 819.109 kb on - strand, within SMa1485 at 819.255 kb on + strand at 819.255 kb on + strand at 819.255 kb on + strand at 819.256 kb on - strand at 819.256 kb on - strand at 819.268 kb on + strand at 819.270 kb on + strand at 819.270 kb on + strand at 819.271 kb on - strand at 819.309 kb on + strand at 819.309 kb on + strand at 819.309 kb on + strand at 819.309 kb on + strand at 819.310 kb on - strand at 819.341 kb on + strand at 819.372 kb on - strand at 819.372 kb on - strand at 819.372 kb on - strand at 819.424 kb on - strand at 819.424 kb on - strand at 819.485 kb on + strand at 819.485 kb on + strand at 819.485 kb on + strand at 819.495 kb on + strand at 819.630 kb on + strand, within SMa1487 at 819.631 kb on - strand, within SMa1487 at 819.631 kb on - strand, within SMa1487 at 819.644 kb on + strand, within SMa1487 at 819.644 kb on + strand, within SMa1487 at 819.645 kb on - strand, within SMa1487 at 820.054 kb on + strand at 820.084 kb on + strand, within SMa1488 at 820.084 kb on + strand, within SMa1488 at 820.085 kb on - strand, within SMa1488
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 817,187 + SMa1483 0.85 +0.2 817,188 - SMa1483 0.85 +0.8 817,404 - +0.7 817,406 - -2.2 817,597 - +4.8 817,731 + +1.1 817,731 + SMa5009 0.32 +1.5 817,732 - SMa5009 0.32 +0.3 817,761 + SMa5009 0.40 -0.3 817,762 - SMa5009 0.41 +0.8 817,762 - SMa5009 0.41 -1.8 817,762 - SMa5009 0.41 +0.5 817,764 + SMa5009 0.41 -0.3 817,764 + SMa5009 0.41 +0.4 817,764 + SMa5009 0.41 -0.3 817,764 + SMa5009 0.41 -0.5 817,764 + SMa5009 0.41 +0.1 817,764 + SMa5009 0.41 +0.4 817,764 + SMa5009 0.41 -1.0 817,765 - SMa5009 0.42 +0.6 817,834 - SMa5009 0.61 -0.4 817,858 - SMa5009 0.68 -0.7 817,858 - SMa5009 0.68 -0.1 817,973 - +1.3 818,420 + SMa1485 0.35 -0.5 818,420 + SMa1485 0.35 -1.5 818,753 + SMa1485 0.61 +1.3 818,754 - SMa1485 0.61 -2.0 818,754 - SMa1485 0.61 -0.6 818,940 - SMa1485 0.76 +0.1 819,108 + SMa1485 0.89 -1.9 819,109 - SMa1485 0.89 +0.6 819,255 + -0.8 819,255 + -1.0 819,255 + -2.5 819,256 - -0.7 819,256 - -1.0 819,268 + +0.2 819,270 + -0.5 819,270 + -0.8 819,271 - +0.2 819,309 + +0.1 819,309 + +2.2 819,309 + -0.5 819,309 + -0.1 819,310 - -1.3 819,341 + +0.3 819,372 - -0.1 819,372 - -1.7 819,372 - +0.3 819,424 - -0.7 819,424 - -0.5 819,485 + +0.3 819,485 + -0.6 819,485 + +0.2 819,495 + -0.0 819,630 + SMa1487 0.40 -0.5 819,631 - SMa1487 0.40 -0.4 819,631 - SMa1487 0.40 -0.8 819,644 + SMa1487 0.43 +1.1 819,644 + SMa1487 0.43 +0.3 819,645 - SMa1487 0.44 +2.1 820,054 + -0.1 820,084 + SMa1488 0.10 +0.4 820,084 + SMa1488 0.10 -0.0 820,085 - SMa1488 0.11 +0.1
Or see this region's nucleotide sequence