Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1306 and SMa1308 overlap by 1 nucleotides SMa1308 and SMa1310 are separated by 4 nucleotides SMa1310 and SMa1311 overlap by 4 nucleotides SMa1311 and SMa1313 are separated by 229 nucleotides SMa1313 and SMa1315 overlap by 1 nucleotides
SMa1306: SMa1306 - VirB9 type IV secretion protein, at 713,420 to 714,274
SMa1306
SMa1308: SMa1308 - VirB8 type IV secretion protein, at 714,274 to 714,945
SMa1308
SMa1310: SMa1310 - VirB7-like protein, at 714,950 to 715,264
SMa1310
SMa1311: SMa1311 - VirB6 type IV secretion protein, at 715,261 to 716,202
SMa1311
SMa1313: SMa1313 - VirB5 type IV secretion protein, at 716,432 to 717,133
SMa1313
SMa1315: SMa1315 - VirB4 type IV secretion protein, at 717,133 to 719,511
SMa1315
Position (kb) 715
716
717 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 714.266 kb on - strand at 714.368 kb on + strand, within SMa1308 at 714.437 kb on + strand, within SMa1308 at 714.516 kb on + strand, within SMa1308 at 714.516 kb on + strand, within SMa1308 at 714.633 kb on - strand, within SMa1308 at 714.633 kb on - strand, within SMa1308 at 714.653 kb on + strand, within SMa1308 at 714.653 kb on + strand, within SMa1308 at 714.832 kb on + strand, within SMa1308 at 714.917 kb on + strand at 714.917 kb on + strand at 714.917 kb on + strand at 714.918 kb on - strand at 714.970 kb on - strand at 715.091 kb on - strand, within SMa1310 at 715.223 kb on - strand, within SMa1310 at 715.223 kb on - strand, within SMa1310 at 715.392 kb on - strand, within SMa1311 at 715.392 kb on - strand, within SMa1311 at 715.445 kb on + strand, within SMa1311 at 715.445 kb on + strand, within SMa1311 at 715.446 kb on - strand, within SMa1311 at 715.446 kb on - strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.455 kb on - strand, within SMa1311 at 715.455 kb on - strand, within SMa1311 at 715.455 kb on - strand, within SMa1311 at 715.509 kb on + strand, within SMa1311 at 715.509 kb on + strand, within SMa1311 at 715.509 kb on + strand, within SMa1311 at 715.510 kb on - strand, within SMa1311 at 715.510 kb on - strand, within SMa1311 at 715.556 kb on - strand, within SMa1311 at 715.722 kb on + strand, within SMa1311 at 715.722 kb on + strand, within SMa1311 at 715.725 kb on - strand, within SMa1311 at 715.943 kb on + strand, within SMa1311 at 715.943 kb on + strand, within SMa1311 at 715.943 kb on + strand, within SMa1311 at 715.944 kb on - strand, within SMa1311 at 715.944 kb on - strand, within SMa1311 at 716.061 kb on - strand, within SMa1311 at 716.061 kb on - strand, within SMa1311 at 716.072 kb on + strand, within SMa1311 at 716.192 kb on + strand at 716.192 kb on + strand at 716.192 kb on + strand at 716.192 kb on + strand at 716.193 kb on - strand at 716.193 kb on - strand at 716.193 kb on - strand at 716.365 kb on + strand at 716.366 kb on - strand at 716.366 kb on - strand at 716.402 kb on + strand at 716.402 kb on + strand at 716.547 kb on - strand, within SMa1313 at 716.679 kb on + strand, within SMa1313 at 716.745 kb on + strand, within SMa1313 at 716.745 kb on + strand, within SMa1313 at 716.773 kb on - strand, within SMa1313 at 716.779 kb on + strand, within SMa1313 at 716.779 kb on + strand, within SMa1313 at 716.780 kb on - strand, within SMa1313 at 717.116 kb on - strand at 717.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 714,266 - -0.1 714,368 + SMa1308 0.14 +1.2 714,437 + SMa1308 0.24 -0.5 714,516 + SMa1308 0.36 -2.2 714,516 + SMa1308 0.36 -1.3 714,633 - SMa1308 0.53 +0.3 714,633 - SMa1308 0.53 +0.8 714,653 + SMa1308 0.56 +0.1 714,653 + SMa1308 0.56 -0.4 714,832 + SMa1308 0.83 +0.9 714,917 + +0.2 714,917 + -0.2 714,917 + +0.1 714,918 - +1.7 714,970 - +0.7 715,091 - SMa1310 0.45 -0.2 715,223 - SMa1310 0.87 +0.6 715,223 - SMa1310 0.87 +0.2 715,392 - SMa1311 0.14 -1.7 715,392 - SMa1311 0.14 -1.5 715,445 + SMa1311 0.20 -1.6 715,445 + SMa1311 0.20 -0.3 715,446 - SMa1311 0.20 -0.5 715,446 - SMa1311 0.20 -0.3 715,454 + SMa1311 0.20 +0.2 715,454 + SMa1311 0.20 +0.1 715,454 + SMa1311 0.20 -1.0 715,454 + SMa1311 0.20 +0.9 715,454 + SMa1311 0.20 +1.6 715,455 - SMa1311 0.21 -0.7 715,455 - SMa1311 0.21 -0.1 715,455 - SMa1311 0.21 -0.7 715,509 + SMa1311 0.26 -2.2 715,509 + SMa1311 0.26 -0.5 715,509 + SMa1311 0.26 +0.5 715,510 - SMa1311 0.26 -0.5 715,510 - SMa1311 0.26 +0.4 715,556 - SMa1311 0.31 +0.2 715,722 + SMa1311 0.49 +0.0 715,722 + SMa1311 0.49 +0.0 715,725 - SMa1311 0.49 -0.1 715,943 + SMa1311 0.72 -1.3 715,943 + SMa1311 0.72 -0.7 715,943 + SMa1311 0.72 +1.1 715,944 - SMa1311 0.73 -1.8 715,944 - SMa1311 0.73 -0.9 716,061 - SMa1311 0.85 -0.4 716,061 - SMa1311 0.85 +0.5 716,072 + SMa1311 0.86 +2.1 716,192 + -0.1 716,192 + +2.0 716,192 + -1.2 716,192 + -2.2 716,193 - +2.9 716,193 - +1.1 716,193 - +0.9 716,365 + -0.1 716,366 - +0.5 716,366 - +0.6 716,402 + +0.9 716,402 + -1.8 716,547 - SMa1313 0.16 +3.2 716,679 + SMa1313 0.35 +0.5 716,745 + SMa1313 0.45 -0.0 716,745 + SMa1313 0.45 +0.1 716,773 - SMa1313 0.49 +0.7 716,779 + SMa1313 0.49 -0.1 716,779 + SMa1313 0.49 +0.3 716,780 - SMa1313 0.50 -0.8 717,116 - +1.1 717,116 - -0.2
Or see this region's nucleotide sequence