Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0144 and SMa0146 are separated by 5 nucleotides SMa0146 and SMa0148 are separated by 140 nucleotides SMa0148 and SMa0149 are separated by 184 nucleotides SMa0149 and SMa0150 are separated by 33 nucleotides
SMa0144: SMa0144 - hypothetical protein, at 79,504 to 80,289
SMa0144
SMa0146: SMa0146 - hypothetical protein, at 80,295 to 80,819
SMa0146
SMa0148: SMa0148 - hypothetical protein, at 80,960 to 81,673
SMa0148
SMa0149: SMa0149 - transcription elongation factor regulatory protein, at 81,858 to 82,340
SMa0149
SMa0150: SMa0150 - malonyl-CoA synthase, at 82,374 to 83,900
SMa0150
Position (kb) 80
81
82 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.378 kb on + strand, within SMa0146 at 80.379 kb on - strand, within SMa0146 at 80.464 kb on + strand, within SMa0146 at 80.465 kb on - strand, within SMa0146 at 80.465 kb on - strand, within SMa0146 at 80.540 kb on + strand, within SMa0146 at 80.541 kb on - strand, within SMa0146 at 80.658 kb on + strand, within SMa0146 at 80.658 kb on + strand, within SMa0146 at 80.855 kb on - strand at 80.942 kb on + strand at 80.943 kb on - strand at 80.943 kb on - strand at 80.943 kb on - strand at 81.101 kb on + strand, within SMa0148 at 81.110 kb on + strand, within SMa0148 at 81.111 kb on - strand, within SMa0148 at 81.179 kb on - strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.298 kb on - strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.669 kb on - strand at 81.669 kb on - strand at 81.723 kb on + strand at 81.724 kb on - strand at 81.724 kb on - strand at 81.775 kb on + strand at 81.775 kb on + strand at 81.776 kb on - strand at 81.786 kb on + strand at 81.786 kb on + strand at 81.788 kb on + strand at 81.790 kb on + strand at 81.790 kb on + strand at 81.791 kb on - strand at 81.791 kb on - strand at 81.791 kb on - strand at 81.822 kb on + strand at 81.823 kb on - strand at 81.823 kb on - strand at 82.081 kb on - strand, within SMa0149 at 82.139 kb on - strand, within SMa0149 at 82.245 kb on + strand, within SMa0149 at 82.245 kb on + strand, within SMa0149 at 82.246 kb on - strand, within SMa0149 at 82.246 kb on - strand, within SMa0149 at 82.339 kb on - strand at 82.411 kb on + strand at 82.411 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.426 kb on - strand at 82.451 kb on + strand at 82.451 kb on + strand at 82.452 kb on - strand at 82.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 80,287 + +0.5 80,287 + -1.3 80,287 + +0.4 80,287 + -1.2 80,287 + +0.3 80,287 + +0.4 80,287 + -1.2 80,287 + +1.2 80,378 + SMa0146 0.16 +1.0 80,379 - SMa0146 0.16 +0.6 80,464 + SMa0146 0.32 +0.5 80,465 - SMa0146 0.32 -1.4 80,465 - SMa0146 0.32 +1.0 80,540 + SMa0146 0.47 +0.2 80,541 - SMa0146 0.47 +0.6 80,658 + SMa0146 0.69 -0.5 80,658 + SMa0146 0.69 -0.2 80,855 - -0.7 80,942 + +0.5 80,943 - -0.2 80,943 - +0.0 80,943 - -0.3 81,101 + SMa0148 0.20 -1.2 81,110 + SMa0148 0.21 -1.0 81,111 - SMa0148 0.21 +0.2 81,179 - SMa0148 0.31 -0.3 81,189 + SMa0148 0.32 +0.4 81,189 + SMa0148 0.32 -1.8 81,189 + SMa0148 0.32 +0.5 81,189 + SMa0148 0.32 +0.1 81,189 + SMa0148 0.32 +0.5 81,190 - SMa0148 0.32 +0.2 81,190 - SMa0148 0.32 -3.4 81,190 - SMa0148 0.32 -0.8 81,190 - SMa0148 0.32 -1.8 81,190 - SMa0148 0.32 -0.5 81,190 - SMa0148 0.32 +0.4 81,190 - SMa0148 0.32 -0.4 81,190 - SMa0148 0.32 -0.3 81,298 - SMa0148 0.47 -1.2 81,472 + SMa0148 0.72 -3.6 81,472 + SMa0148 0.72 +2.2 81,472 + SMa0148 0.72 -2.4 81,472 + SMa0148 0.72 +0.1 81,669 - -0.3 81,669 - -0.8 81,723 + -2.3 81,724 - -2.0 81,724 - +0.5 81,775 + -0.5 81,775 + -0.4 81,776 - -1.0 81,786 + -2.0 81,786 + -1.6 81,788 + +0.6 81,790 + +0.0 81,790 + -2.6 81,791 - -0.6 81,791 - -1.1 81,791 - -0.9 81,822 + +1.5 81,823 - +0.1 81,823 - +0.1 82,081 - SMa0149 0.46 -0.4 82,139 - SMa0149 0.58 -0.3 82,245 + SMa0149 0.80 -1.2 82,245 + SMa0149 0.80 +1.2 82,246 - SMa0149 0.80 +0.5 82,246 - SMa0149 0.80 -0.6 82,339 - -0.3 82,411 + -1.0 82,411 + +0.4 82,425 + -3.9 82,425 + -0.1 82,426 - -0.2 82,451 + +1.7 82,451 + -2.7 82,452 - -2.4 82,498 + -1.0
Or see this region's nucleotide sequence