Experiment: Paraquat dichloride 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21122 and SM_b21123 are separated by 14 nucleotides SM_b21123 and SM_b21124 are separated by 16 nucleotides SM_b21124 and SM_b21125 overlap by 4 nucleotides SM_b21125 and SM_b21126 overlap by 4 nucleotides
SM_b21122: SM_b21122 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (from data) , at 760,760 to 762,367
_b21122
SM_b21123: SM_b21123 - translation initiation inhibitor, at 762,382 to 762,765
_b21123
SM_b21124: SM_b21124 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 762,782 to 764,770
_b21124
SM_b21125: SM_b21125 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) (from data) , at 764,767 to 765,636
_b21125
SM_b21126: SM_b21126 - 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) (from data) , at 765,633 to 766,418
_b21126
Position (kb)
762
763
764
765 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 761.912 kb on + strand, within SM_b21122 at 762.023 kb on + strand, within SM_b21122 at 762.210 kb on + strand at 762.211 kb on - strand at 762.236 kb on + strand at 762.237 kb on - strand at 762.237 kb on - strand at 762.497 kb on + strand, within SM_b21123 at 762.670 kb on + strand, within SM_b21123 at 762.670 kb on + strand, within SM_b21123 at 762.671 kb on - strand, within SM_b21123 at 762.763 kb on + strand at 762.763 kb on + strand at 762.764 kb on - strand at 762.782 kb on - strand at 762.782 kb on - strand at 762.959 kb on + strand at 762.977 kb on - strand at 763.083 kb on + strand, within SM_b21124 at 763.083 kb on + strand, within SM_b21124 at 763.083 kb on + strand, within SM_b21124 at 763.084 kb on - strand, within SM_b21124 at 763.084 kb on - strand, within SM_b21124 at 763.441 kb on + strand, within SM_b21124 at 763.497 kb on + strand, within SM_b21124 at 763.583 kb on + strand, within SM_b21124 at 763.649 kb on + strand, within SM_b21124 at 763.745 kb on - strand, within SM_b21124 at 763.777 kb on + strand, within SM_b21124 at 763.778 kb on - strand, within SM_b21124 at 763.815 kb on - strand, within SM_b21124 at 763.815 kb on - strand, within SM_b21124 at 763.921 kb on + strand, within SM_b21124 at 763.937 kb on + strand, within SM_b21124 at 763.937 kb on + strand, within SM_b21124 at 763.965 kb on + strand, within SM_b21124 at 763.965 kb on + strand, within SM_b21124 at 763.966 kb on - strand, within SM_b21124 at 764.029 kb on - strand, within SM_b21124 at 764.105 kb on - strand, within SM_b21124 at 765.021 kb on + strand, within SM_b21125 at 765.021 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.194 kb on - strand, within SM_b21125 at 765.221 kb on - strand, within SM_b21125 at 765.221 kb on - strand, within SM_b21125 at 765.287 kb on - strand, within SM_b21125 at 765.402 kb on + strand, within SM_b21125 at 765.402 kb on + strand, within SM_b21125 at 765.403 kb on - strand, within SM_b21125 at 765.496 kb on + strand, within SM_b21125 at 765.526 kb on - strand, within SM_b21125
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.2 mg/ml remove 761,912 + SM_b21122 0.72 -1.3 762,023 + SM_b21122 0.79 +0.4 762,210 + +0.7 762,211 - +0.1 762,236 + +1.2 762,237 - -1.2 762,237 - +0.4 762,497 + SM_b21123 0.30 -1.6 762,670 + SM_b21123 0.75 +0.4 762,670 + SM_b21123 0.75 -0.3 762,671 - SM_b21123 0.75 -0.2 762,763 + -0.3 762,763 + -0.0 762,764 - -0.2 762,782 - +0.6 762,782 - +0.4 762,959 + +0.1 762,977 - -1.0 763,083 + SM_b21124 0.15 -2.8 763,083 + SM_b21124 0.15 +0.5 763,083 + SM_b21124 0.15 -1.6 763,084 - SM_b21124 0.15 -0.7 763,084 - SM_b21124 0.15 +1.0 763,441 + SM_b21124 0.33 +0.7 763,497 + SM_b21124 0.36 +0.1 763,583 + SM_b21124 0.40 +0.6 763,649 + SM_b21124 0.44 +0.1 763,745 - SM_b21124 0.48 -0.9 763,777 + SM_b21124 0.50 -1.6 763,778 - SM_b21124 0.50 +0.4 763,815 - SM_b21124 0.52 -1.1 763,815 - SM_b21124 0.52 -0.6 763,921 + SM_b21124 0.57 +0.3 763,937 + SM_b21124 0.58 -0.4 763,937 + SM_b21124 0.58 +0.3 763,965 + SM_b21124 0.59 +0.4 763,965 + SM_b21124 0.59 -1.6 763,966 - SM_b21124 0.60 -0.6 764,029 - SM_b21124 0.63 +3.3 764,105 - SM_b21124 0.67 +1.0 765,021 + SM_b21125 0.29 +0.7 765,021 + SM_b21125 0.29 -0.6 765,193 + SM_b21125 0.49 +1.0 765,193 + SM_b21125 0.49 -1.1 765,193 + SM_b21125 0.49 -0.4 765,194 - SM_b21125 0.49 -0.6 765,221 - SM_b21125 0.52 -1.6 765,221 - SM_b21125 0.52 -1.4 765,287 - SM_b21125 0.60 -1.0 765,402 + SM_b21125 0.73 -0.3 765,402 + SM_b21125 0.73 +0.7 765,403 - SM_b21125 0.73 -0.3 765,496 + SM_b21125 0.84 -1.0 765,526 - SM_b21125 0.87 +0.1
Or see this region's nucleotide sequence