Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_693 and Pf1N1B4_694 are separated by 293 nucleotides Pf1N1B4_694 and Pf1N1B4_695 are separated by 218 nucleotides
Pf1N1B4_693 - ABC spermidine/putrescine transporter, inner membrane subunit, at 791,111 to 791,917
_693
Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314
_694
Pf1N1B4_695 - Aldehyde dehydrogenase (EC 1.2.1.3), at 793,533 to 794,981
_695
Position (kb) 792
793
794 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 791.433 kb on + strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.712 kb on + strand, within Pf1N1B4_693 at 791.712 kb on + strand, within Pf1N1B4_693 at 791.713 kb on - strand, within Pf1N1B4_693 at 791.793 kb on + strand at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand at 791.817 kb on + strand, within Pf1N1B4_693 at 791.877 kb on - strand at 792.113 kb on + strand at 792.113 kb on + strand at 792.187 kb on + strand at 792.187 kb on + strand at 792.188 kb on - strand at 792.188 kb on - strand at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand at 792.380 kb on + strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.441 kb on - strand, within Pf1N1B4_694 at 792.441 kb on - strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.501 kb on - strand, within Pf1N1B4_694 at 792.539 kb on + strand, within Pf1N1B4_694 at 792.540 kb on - strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.582 kb on - strand, within Pf1N1B4_694 at 792.666 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.855 kb on - strand, within Pf1N1B4_694 at 793.025 kb on + strand, within Pf1N1B4_694 at 793.109 kb on + strand, within Pf1N1B4_694 at 793.110 kb on - strand, within Pf1N1B4_694 at 793.110 kb on - strand, within Pf1N1B4_694 at 793.337 kb on - strand at 793.337 kb on - strand at 793.337 kb on - strand at 793.340 kb on + strand at 793.340 kb on + strand at 793.340 kb on + strand at 793.341 kb on - strand at 793.341 kb on - strand at 793.801 kb on - strand, within Pf1N1B4_695 at 793.914 kb on - strand, within Pf1N1B4_695 at 794.196 kb on + strand, within Pf1N1B4_695
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 791,433 + Pf1N1B4_693 0.40 -0.7 791,530 - Pf1N1B4_693 0.52 +0.6 791,530 - Pf1N1B4_693 0.52 -0.5 791,530 - Pf1N1B4_693 0.52 -0.7 791,530 - Pf1N1B4_693 0.52 -0.3 791,712 + Pf1N1B4_693 0.74 -0.5 791,712 + Pf1N1B4_693 0.74 -0.4 791,713 - Pf1N1B4_693 0.75 -0.3 791,793 + +0.7 791,797 - Pf1N1B4_693 0.85 -0.0 791,797 - Pf1N1B4_693 0.85 +0.7 791,797 - Pf1N1B4_693 0.85 -1.3 791,797 - Pf1N1B4_693 0.85 -0.1 791,797 - Pf1N1B4_693 0.85 -0.9 791,797 - +1.3 791,817 + Pf1N1B4_693 0.87 -1.9 791,877 - -0.0 792,113 + -0.9 792,113 + -1.1 792,187 + -0.2 792,187 + +0.2 792,188 - +0.7 792,188 - +0.7 792,323 + Pf1N1B4_694 0.10 +0.5 792,323 + Pf1N1B4_694 0.10 -0.5 792,323 + Pf1N1B4_694 0.10 +1.3 792,323 + Pf1N1B4_694 0.10 -0.9 792,324 - Pf1N1B4_694 0.10 +2.3 792,324 - Pf1N1B4_694 0.10 -0.3 792,324 - Pf1N1B4_694 0.10 +1.7 792,324 - Pf1N1B4_694 0.10 +0.7 792,324 - Pf1N1B4_694 0.10 +0.0 792,380 + Pf1N1B4_694 0.15 -1.1 792,380 + Pf1N1B4_694 0.15 -1.7 792,380 + Pf1N1B4_694 0.15 -1.9 792,380 + Pf1N1B4_694 0.15 -0.9 792,380 + -1.3 792,380 + Pf1N1B4_694 0.15 -0.1 792,381 - Pf1N1B4_694 0.15 -0.3 792,381 - Pf1N1B4_694 0.15 +0.1 792,381 - Pf1N1B4_694 0.15 +0.5 792,441 - Pf1N1B4_694 0.21 -1.5 792,441 - Pf1N1B4_694 0.21 -2.8 792,500 + Pf1N1B4_694 0.26 +1.1 792,500 + Pf1N1B4_694 0.26 -0.2 792,500 + Pf1N1B4_694 0.26 -1.2 792,501 - Pf1N1B4_694 0.26 +0.7 792,539 + Pf1N1B4_694 0.30 -0.7 792,540 - Pf1N1B4_694 0.30 -0.8 792,581 + Pf1N1B4_694 0.34 +2.5 792,581 + Pf1N1B4_694 0.34 -2.1 792,582 - Pf1N1B4_694 0.34 +0.7 792,666 + Pf1N1B4_694 0.41 -0.9 792,818 + Pf1N1B4_694 0.55 -0.1 792,818 + Pf1N1B4_694 0.55 -0.3 792,818 + Pf1N1B4_694 0.55 +0.2 792,818 + Pf1N1B4_694 0.55 +1.1 792,819 - Pf1N1B4_694 0.55 -0.2 792,819 - Pf1N1B4_694 0.55 -1.2 792,855 - Pf1N1B4_694 0.58 -0.1 793,025 + Pf1N1B4_694 0.74 -0.3 793,109 + Pf1N1B4_694 0.81 +0.3 793,110 - Pf1N1B4_694 0.81 +0.9 793,110 - Pf1N1B4_694 0.81 -2.9 793,337 - +0.9 793,337 - -1.3 793,337 - +0.1 793,340 + -0.4 793,340 + -1.0 793,340 + +1.3 793,341 - -0.4 793,341 - -0.6 793,801 - Pf1N1B4_695 0.18 -0.3 793,914 - Pf1N1B4_695 0.26 -1.1 794,196 + Pf1N1B4_695 0.46 -0.9
Or see this region's nucleotide sequence