Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotides Pf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotides Pf1N1B4_68 and Pf1N1B4_69 are separated by 293 nucleotides
Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353
_66
Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186
_67
Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535
_68
Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542
_69
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.271 kb on + strand, within Pf1N1B4_66 at 69.330 kb on + strand at 69.330 kb on + strand at 69.395 kb on + strand at 69.432 kb on + strand at 69.432 kb on + strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.473 kb on + strand at 69.474 kb on - strand at 69.474 kb on - strand at 69.476 kb on - strand at 69.476 kb on - strand at 69.639 kb on - strand, within Pf1N1B4_67 at 69.729 kb on + strand, within Pf1N1B4_67 at 69.798 kb on - strand, within Pf1N1B4_67 at 70.129 kb on + strand at 70.160 kb on + strand at 70.161 kb on - strand at 70.534 kb on + strand, within Pf1N1B4_68 at 70.596 kb on - strand, within Pf1N1B4_68 at 71.044 kb on + strand, within Pf1N1B4_68 at 71.044 kb on + strand, within Pf1N1B4_68 at 71.045 kb on - strand, within Pf1N1B4_68 at 71.045 kb on - strand, within Pf1N1B4_68 at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.567 kb on - strand at 71.569 kb on + strand at 71.569 kb on + strand at 71.570 kb on - strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.862 kb on - strand at 71.871 kb on + strand at 71.872 kb on - strand at 72.028 kb on - strand, within Pf1N1B4_69 at 72.028 kb on - strand, within Pf1N1B4_69 at 72.139 kb on + strand, within Pf1N1B4_69 at 72.140 kb on - strand, within Pf1N1B4_69 at 72.303 kb on + strand, within Pf1N1B4_69 at 72.304 kb on - strand, within Pf1N1B4_69
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 69,207 - Pf1N1B4_66 0.73 +0.1 69,207 - Pf1N1B4_66 0.73 -0.5 69,271 + Pf1N1B4_66 0.85 -1.2 69,330 + -0.0 69,330 + +1.2 69,395 + +1.1 69,432 + -0.0 69,432 + -0.7 69,433 - -1.0 69,433 - +0.7 69,433 - -0.0 69,433 - -0.3 69,473 + -0.3 69,474 - -1.9 69,474 - -0.6 69,476 - -1.0 69,476 - +0.4 69,639 - Pf1N1B4_67 0.20 +0.6 69,729 + Pf1N1B4_67 0.33 -1.5 69,798 - Pf1N1B4_67 0.43 -0.2 70,129 + -1.2 70,160 + -1.6 70,161 - +1.4 70,534 + Pf1N1B4_68 0.26 -1.2 70,596 - Pf1N1B4_68 0.30 -2.6 71,044 + Pf1N1B4_68 0.64 -2.2 71,044 + Pf1N1B4_68 0.64 -0.1 71,045 - Pf1N1B4_68 0.64 -1.2 71,045 - Pf1N1B4_68 0.64 -1.2 71,566 + -1.2 71,566 + +0.1 71,566 + +0.4 71,566 + +0.4 71,566 + -0.1 71,566 + -0.6 71,566 + -2.4 71,567 - +0.6 71,569 + -0.3 71,569 + +0.4 71,570 - +0.0 71,722 + +0.5 71,722 + -2.4 71,722 + -0.0 71,722 + +0.2 71,862 - -0.5 71,871 + -0.0 71,872 - -0.6 72,028 - Pf1N1B4_69 0.28 -1.4 72,028 - Pf1N1B4_69 0.28 -0.2 72,139 + Pf1N1B4_69 0.43 +1.0 72,140 - Pf1N1B4_69 0.44 -2.4 72,303 + Pf1N1B4_69 0.66 -0.2 72,304 - Pf1N1B4_69 0.67 -0.6
Or see this region's nucleotide sequence