Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_65 and Pf1N1B4_66 are separated by 61 nucleotides Pf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotides Pf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotides
Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755
_65
Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353
_66
Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186
_67
Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535
_68
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 67.838 kb on + strand at 67.838 kb on + strand at 67.839 kb on - strand at 67.839 kb on - strand at 67.923 kb on + strand at 67.923 kb on + strand at 67.923 kb on + strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.927 kb on - strand at 67.927 kb on - strand at 68.011 kb on - strand, within Pf1N1B4_65 at 68.011 kb on - strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.122 kb on - strand, within Pf1N1B4_65 at 68.122 kb on - strand, within Pf1N1B4_65 at 68.232 kb on - strand, within Pf1N1B4_65 at 68.245 kb on + strand, within Pf1N1B4_65 at 68.246 kb on - strand at 68.292 kb on + strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.484 kb on + strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.619 kb on + strand, within Pf1N1B4_65 at 68.619 kb on + strand, within Pf1N1B4_65 at 68.619 kb on + strand at 68.620 kb on - strand, within Pf1N1B4_65 at 68.620 kb on - strand, within Pf1N1B4_65 at 68.698 kb on - strand at 68.698 kb on - strand at 68.709 kb on + strand at 68.710 kb on - strand at 68.871 kb on - strand, within Pf1N1B4_66 at 69.038 kb on + strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand at 69.045 kb on - strand, within Pf1N1B4_66 at 69.129 kb on + strand, within Pf1N1B4_66 at 69.130 kb on - strand, within Pf1N1B4_66 at 69.130 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand at 69.150 kb on + strand, within Pf1N1B4_66 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.271 kb on + strand, within Pf1N1B4_66 at 69.330 kb on + strand at 69.330 kb on + strand at 69.395 kb on + strand at 69.432 kb on + strand at 69.432 kb on + strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.473 kb on + strand at 69.474 kb on - strand at 69.474 kb on - strand at 69.476 kb on - strand at 69.476 kb on - strand at 69.639 kb on - strand, within Pf1N1B4_67 at 69.729 kb on + strand, within Pf1N1B4_67 at 69.798 kb on - strand, within Pf1N1B4_67 at 70.129 kb on + strand at 70.160 kb on + strand at 70.161 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 67,838 + -1.2 67,838 + +0.1 67,839 - -0.6 67,839 - -1.2 67,923 + +0.6 67,923 + -1.2 67,923 + -1.3 67,924 - -1.6 67,924 - -0.7 67,924 - +1.2 67,924 - -0.9 67,924 - -1.8 67,924 - +1.1 67,924 - -1.3 67,924 - +0.4 67,927 - +0.4 67,927 - -0.1 68,011 - Pf1N1B4_65 0.10 +1.4 68,011 - Pf1N1B4_65 0.10 +0.6 68,019 + Pf1N1B4_65 0.11 +1.4 68,019 + Pf1N1B4_65 0.11 +2.7 68,019 + Pf1N1B4_65 0.11 +0.4 68,020 - Pf1N1B4_65 0.11 -1.6 68,020 - Pf1N1B4_65 0.11 +1.1 68,020 - Pf1N1B4_65 0.11 -1.1 68,020 - Pf1N1B4_65 0.11 -1.6 68,122 - Pf1N1B4_65 0.24 -1.9 68,122 - Pf1N1B4_65 0.24 +0.8 68,232 - Pf1N1B4_65 0.37 -1.2 68,245 + Pf1N1B4_65 0.39 +1.4 68,246 - +2.0 68,292 + Pf1N1B4_65 0.44 +1.4 68,293 - Pf1N1B4_65 0.44 -0.2 68,293 - Pf1N1B4_65 0.44 -1.4 68,293 - Pf1N1B4_65 0.44 -0.6 68,293 - Pf1N1B4_65 0.44 +1.3 68,293 - Pf1N1B4_65 0.44 +0.4 68,293 - Pf1N1B4_65 0.44 +0.4 68,484 + Pf1N1B4_65 0.67 +1.3 68,485 - Pf1N1B4_65 0.67 +1.0 68,485 - Pf1N1B4_65 0.67 +0.4 68,485 - Pf1N1B4_65 0.67 +0.5 68,619 + Pf1N1B4_65 0.84 +2.5 68,619 + Pf1N1B4_65 0.84 +1.4 68,619 + +2.0 68,620 - Pf1N1B4_65 0.84 +2.0 68,620 - Pf1N1B4_65 0.84 +1.0 68,698 - +2.0 68,698 - +0.8 68,709 + +1.0 68,710 - +1.8 68,871 - Pf1N1B4_66 0.10 -1.2 69,038 + Pf1N1B4_66 0.41 -0.8 69,045 - Pf1N1B4_66 0.42 +0.6 69,045 - Pf1N1B4_66 0.42 -1.2 69,045 - Pf1N1B4_66 0.42 -0.5 69,045 - Pf1N1B4_66 0.42 +0.8 69,045 - -1.6 69,045 - Pf1N1B4_66 0.42 -0.2 69,129 + Pf1N1B4_66 0.58 -0.9 69,130 - Pf1N1B4_66 0.58 +0.4 69,130 - Pf1N1B4_66 0.58 -0.3 69,141 - Pf1N1B4_66 0.60 -0.1 69,141 - Pf1N1B4_66 0.60 +0.2 69,141 - Pf1N1B4_66 0.60 -0.9 69,141 - Pf1N1B4_66 0.60 +2.4 69,141 - -1.2 69,150 + Pf1N1B4_66 0.62 -0.5 69,207 - Pf1N1B4_66 0.73 +0.1 69,207 - Pf1N1B4_66 0.73 -0.5 69,271 + Pf1N1B4_66 0.85 -1.2 69,330 + -0.0 69,330 + +1.2 69,395 + +1.1 69,432 + -0.0 69,432 + -0.7 69,433 - -1.0 69,433 - +0.7 69,433 - -0.0 69,433 - -0.3 69,473 + -0.3 69,474 - -1.9 69,474 - -0.6 69,476 - -1.0 69,476 - +0.4 69,639 - Pf1N1B4_67 0.20 +0.6 69,729 + Pf1N1B4_67 0.33 -1.5 69,798 - Pf1N1B4_67 0.43 -0.2 70,129 + -1.2 70,160 + -1.6 70,161 - +1.4
Or see this region's nucleotide sequence