Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_615 and Pf1N1B4_616 are separated by 69 nucleotides Pf1N1B4_616 and Pf1N1B4_617 are separated by 191 nucleotides
Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472
_615
Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552
_616
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Position (kb) 712
713
714 Strain fitness (log2 ratio) -2
-1
0
1
2 at 711.756 kb on + strand, within Pf1N1B4_615 at 711.757 kb on - strand, within Pf1N1B4_615 at 712.344 kb on + strand at 712.344 kb on + strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.467 kb on + strand at 712.468 kb on - strand at 712.608 kb on + strand at 712.609 kb on - strand at 712.647 kb on + strand, within Pf1N1B4_616 at 712.648 kb on - strand, within Pf1N1B4_616 at 712.648 kb on - strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 713.550 kb on + strand at 713.575 kb on + strand at 713.576 kb on - strand at 713.744 kb on - strand at 713.933 kb on + strand, within Pf1N1B4_617 at 713.996 kb on + strand at 714.126 kb on - strand, within Pf1N1B4_617 at 714.126 kb on - strand at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 711,756 + Pf1N1B4_615 0.43 -0.1 711,757 - Pf1N1B4_615 0.43 +1.1 712,344 + -0.4 712,344 + Pf1N1B4_615 0.90 -1.7 712,345 - Pf1N1B4_615 0.90 +0.6 712,345 - Pf1N1B4_615 0.90 -2.5 712,345 - Pf1N1B4_615 0.90 +0.4 712,467 + -0.1 712,468 - -0.3 712,608 + +0.7 712,609 - +2.0 712,647 + Pf1N1B4_616 0.10 +0.4 712,648 - Pf1N1B4_616 0.10 -1.7 712,648 - Pf1N1B4_616 0.10 -0.9 712,770 + Pf1N1B4_616 0.23 -0.3 712,770 + Pf1N1B4_616 0.23 -2.2 712,771 - Pf1N1B4_616 0.23 -0.8 712,771 - Pf1N1B4_616 0.23 +0.7 713,550 + -0.9 713,575 + +0.3 713,576 - -0.1 713,744 - -1.3 713,933 + Pf1N1B4_617 0.20 -1.9 713,996 + -0.3 714,126 - Pf1N1B4_617 0.41 -1.3 714,126 - -0.9 714,315 - Pf1N1B4_617 0.61 -1.2 714,315 - Pf1N1B4_617 0.61 -0.2
Or see this region's nucleotide sequence