Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5891 and Pf1N1B4_5892 overlap by 34 nucleotides Pf1N1B4_5892 and Pf1N1B4_5893 overlap by 4 nucleotides Pf1N1B4_5893 and Pf1N1B4_5894 overlap by 8 nucleotides
Pf1N1B4_5891 - FIG00961753: hypothetical protein, at 334,853 to 336,067
_5891
Pf1N1B4_5892 - FIG00961753: hypothetical protein, at 336,034 to 336,216
_5892
Pf1N1B4_5893 - Mycofactocin system glycosyltransferase, at 336,213 to 337,190
_5893
Pf1N1B4_5894 - Glycosyltransferase, at 337,183 to 338,319
_5894
Position (kb)
336
337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 335.177 kb on + strand, within Pf1N1B4_5891 at 335.299 kb on + strand at 335.682 kb on - strand, within Pf1N1B4_5891 at 335.779 kb on + strand at 335.928 kb on + strand at 335.928 kb on + strand, within Pf1N1B4_5891 at 335.929 kb on - strand, within Pf1N1B4_5891 at 335.929 kb on - strand, within Pf1N1B4_5891 at 335.929 kb on - strand, within Pf1N1B4_5891 at 335.932 kb on + strand, within Pf1N1B4_5891 at 335.932 kb on + strand, within Pf1N1B4_5891 at 336.004 kb on + strand at 336.004 kb on + strand at 336.004 kb on + strand at 336.004 kb on + strand at 336.005 kb on - strand at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.139 kb on + strand, within Pf1N1B4_5892 at 336.252 kb on + strand at 336.252 kb on + strand at 336.279 kb on - strand at 337.015 kb on + strand, within Pf1N1B4_5893 at 337.016 kb on - strand, within Pf1N1B4_5893 at 337.078 kb on + strand, within Pf1N1B4_5893 at 337.079 kb on - strand at 337.079 kb on - strand, within Pf1N1B4_5893
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 335,177 + Pf1N1B4_5891 0.27 -1.1 335,299 + +1.2 335,682 - Pf1N1B4_5891 0.68 -0.8 335,779 + -2.3 335,928 + -1.8 335,928 + Pf1N1B4_5891 0.88 -0.3 335,929 - Pf1N1B4_5891 0.89 +1.8 335,929 - Pf1N1B4_5891 0.89 +1.1 335,929 - Pf1N1B4_5891 0.89 +0.1 335,932 + Pf1N1B4_5891 0.89 -0.8 335,932 + Pf1N1B4_5891 0.89 -0.5 336,004 + -0.5 336,004 + -0.1 336,004 + +2.1 336,004 + +1.2 336,005 - -0.8 336,088 + Pf1N1B4_5892 0.30 +0.2 336,088 + Pf1N1B4_5892 0.30 -0.2 336,088 + Pf1N1B4_5892 0.30 -1.3 336,088 + Pf1N1B4_5892 0.30 -2.6 336,088 + -1.3 336,088 + Pf1N1B4_5892 0.30 -0.4 336,088 + Pf1N1B4_5892 0.30 -3.3 336,089 - Pf1N1B4_5892 0.30 -1.1 336,089 - Pf1N1B4_5892 0.30 +1.0 336,089 - Pf1N1B4_5892 0.30 -2.3 336,089 - Pf1N1B4_5892 0.30 -1.3 336,139 + Pf1N1B4_5892 0.57 -1.1 336,252 + -2.8 336,252 + -1.9 336,279 - +0.5 337,015 + Pf1N1B4_5893 0.82 -0.1 337,016 - Pf1N1B4_5893 0.82 +0.1 337,078 + Pf1N1B4_5893 0.88 -0.2 337,079 - +0.2 337,079 - Pf1N1B4_5893 0.89 +0.2
Or see this region's nucleotide sequence