Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5679 and Pf1N1B4_5680 overlap by 4 nucleotides Pf1N1B4_5680 and Pf1N1B4_5681 are separated by 7 nucleotides Pf1N1B4_5681 and Pf1N1B4_5682 overlap by 1 nucleotides
Pf1N1B4_5679 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 116,795 to 119,872
_5679
Pf1N1B4_5680 - DNA polymerase-like protein PA0670, at 119,869 to 121,284
_5680
Pf1N1B4_5681 - RecA/RadA recombinase, at 121,292 to 121,909
_5681
Pf1N1B4_5682 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 121,909 to 122,526
_5682
Position (kb) 119
120
121
122 Strain fitness (log2 ratio) -2
-1
0
1
2 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.557 kb on - strand, within Pf1N1B4_5679 at 119.667 kb on - strand at 119.960 kb on + strand at 119.961 kb on - strand at 119.964 kb on - strand at 120.098 kb on - strand at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.256 kb on + strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.616 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.676 kb on - strand, within Pf1N1B4_5680 at 120.818 kb on - strand at 120.818 kb on - strand, within Pf1N1B4_5680 at 121.071 kb on - strand, within Pf1N1B4_5680 at 121.986 kb on - strand, within Pf1N1B4_5682 at 122.002 kb on + strand at 122.134 kb on - strand, within Pf1N1B4_5682 at 122.174 kb on + strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 119,418 - Pf1N1B4_5679 0.85 -0.9 119,418 - Pf1N1B4_5679 0.85 -1.4 119,418 - Pf1N1B4_5679 0.85 -2.1 119,557 - Pf1N1B4_5679 0.90 +1.9 119,667 - -0.9 119,960 + +1.0 119,961 - +0.2 119,964 - -0.4 120,098 - -0.8 120,098 - Pf1N1B4_5680 0.16 -0.7 120,098 - Pf1N1B4_5680 0.16 -2.1 120,256 + Pf1N1B4_5680 0.27 -1.4 120,594 - Pf1N1B4_5680 0.51 -1.4 120,594 - Pf1N1B4_5680 0.51 -2.1 120,616 + Pf1N1B4_5680 0.53 -1.2 120,675 + Pf1N1B4_5680 0.57 +0.2 120,675 + Pf1N1B4_5680 0.57 -1.4 120,676 - Pf1N1B4_5680 0.57 -1.0 120,818 - -1.4 120,818 - Pf1N1B4_5680 0.67 -0.4 121,071 - Pf1N1B4_5680 0.85 +0.2 121,986 - Pf1N1B4_5682 0.12 -0.1 122,002 + +2.2 122,134 - Pf1N1B4_5682 0.36 -1.0 122,174 + Pf1N1B4_5682 0.43 +0.8 122,175 - Pf1N1B4_5682 0.43 -1.4 122,175 - Pf1N1B4_5682 0.43 -0.4
Or see this region's nucleotide sequence