Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5645

Experiment: Gentamicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5643 and Pf1N1B4_5644 are separated by 9 nucleotidesPf1N1B4_5644 and Pf1N1B4_5645 are separated by 52 nucleotidesPf1N1B4_5645 and Pf1N1B4_5646 overlap by 6 nucleotidesPf1N1B4_5646 and Pf1N1B4_5647 are separated by 197 nucleotidesPf1N1B4_5647 and Pf1N1B4_5648 are separated by 133 nucleotides Pf1N1B4_5643 - Amino acid ABC transporter, permease protein, at 72,904 to 73,566 _5643 Pf1N1B4_5644 - amino acid ABC transporter, permease protein, at 73,576 to 74,238 _5644 Pf1N1B4_5645 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 74,291 to 75,136 _5645 Pf1N1B4_5646 - hypothetical protein, at 75,131 to 75,280 _5646 Pf1N1B4_5647 - hypothetical protein, at 75,478 to 75,927 _5647 Pf1N1B4_5648 - transcriptional regulator, MerR family, at 76,061 to 76,465 _5648 Position (kb) 74 75 76Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 73.332 kb on - strand, within Pf1N1B4_5643at 73.332 kb on - strand, within Pf1N1B4_5643at 73.352 kb on - strand, within Pf1N1B4_5643at 73.562 kb on + strandat 73.563 kb on - strandat 73.563 kb on - strandat 73.646 kb on + strand, within Pf1N1B4_5644at 73.647 kb on - strand, within Pf1N1B4_5644at 73.647 kb on - strand, within Pf1N1B4_5644at 73.713 kb on - strand, within Pf1N1B4_5644at 73.715 kb on + strand, within Pf1N1B4_5644at 73.716 kb on - strand, within Pf1N1B4_5644at 73.716 kb on - strand, within Pf1N1B4_5644at 73.829 kb on + strand, within Pf1N1B4_5644at 73.829 kb on + strand, within Pf1N1B4_5644at 73.829 kb on + strand, within Pf1N1B4_5644at 73.830 kb on - strand, within Pf1N1B4_5644at 73.940 kb on + strand, within Pf1N1B4_5644at 73.991 kb on - strand, within Pf1N1B4_5644at 74.009 kb on + strand, within Pf1N1B4_5644at 74.010 kb on - strand, within Pf1N1B4_5644at 74.060 kb on + strand, within Pf1N1B4_5644at 74.060 kb on + strandat 74.060 kb on + strand, within Pf1N1B4_5644at 74.231 kb on + strandat 74.232 kb on - strandat 74.232 kb on - strandat 74.248 kb on + strandat 74.248 kb on + strandat 74.249 kb on - strandat 74.249 kb on - strandat 74.367 kb on + strandat 74.461 kb on - strand, within Pf1N1B4_5645at 74.461 kb on - strandat 74.523 kb on + strand, within Pf1N1B4_5645at 74.524 kb on - strandat 74.584 kb on - strand, within Pf1N1B4_5645at 74.656 kb on - strand, within Pf1N1B4_5645at 74.721 kb on + strand, within Pf1N1B4_5645at 74.722 kb on - strand, within Pf1N1B4_5645at 74.722 kb on - strand, within Pf1N1B4_5645at 74.999 kb on - strand, within Pf1N1B4_5645at 75.188 kb on - strand, within Pf1N1B4_5646at 75.577 kb on + strand, within Pf1N1B4_5647at 75.625 kb on + strand, within Pf1N1B4_5647at 75.625 kb on + strand, within Pf1N1B4_5647at 75.625 kb on + strand, within Pf1N1B4_5647at 75.626 kb on - strand, within Pf1N1B4_5647at 75.627 kb on + strand, within Pf1N1B4_5647at 75.627 kb on + strand, within Pf1N1B4_5647at 75.627 kb on + strand, within Pf1N1B4_5647at 75.627 kb on + strand, within Pf1N1B4_5647at 75.627 kb on + strand, within Pf1N1B4_5647at 75.647 kb on - strand, within Pf1N1B4_5647at 75.712 kb on + strand, within Pf1N1B4_5647at 75.869 kb on + strand, within Pf1N1B4_5647at 75.869 kb on + strand, within Pf1N1B4_5647at 76.062 kb on + strandat 76.062 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml
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73,332 - Pf1N1B4_5643 0.65 -1.6
73,332 - Pf1N1B4_5643 0.65 +0.2
73,352 - Pf1N1B4_5643 0.68 -1.4
73,562 + -1.3
73,563 - -1.6
73,563 - +0.7
73,646 + Pf1N1B4_5644 0.11 -0.2
73,647 - Pf1N1B4_5644 0.11 +0.2
73,647 - Pf1N1B4_5644 0.11 +0.2
73,713 - Pf1N1B4_5644 0.21 +0.9
73,715 + Pf1N1B4_5644 0.21 +0.8
73,716 - Pf1N1B4_5644 0.21 +1.2
73,716 - Pf1N1B4_5644 0.21 +1.9
73,829 + Pf1N1B4_5644 0.38 +0.0
73,829 + Pf1N1B4_5644 0.38 +2.8
73,829 + Pf1N1B4_5644 0.38 +0.2
73,830 - Pf1N1B4_5644 0.38 +2.0
73,940 + Pf1N1B4_5644 0.55 -0.8
73,991 - Pf1N1B4_5644 0.63 -1.1
74,009 + Pf1N1B4_5644 0.65 -0.4
74,010 - Pf1N1B4_5644 0.65 -2.0
74,060 + Pf1N1B4_5644 0.73 +0.5
74,060 + -1.4
74,060 + Pf1N1B4_5644 0.73 +0.7
74,231 + +0.0
74,232 - -1.1
74,232 - -1.1
74,248 + +0.3
74,248 + -0.2
74,249 - +0.1
74,249 - -1.0
74,367 + +0.2
74,461 - Pf1N1B4_5645 0.20 -1.6
74,461 - -1.1
74,523 + Pf1N1B4_5645 0.27 -1.7
74,524 - -1.8
74,584 - Pf1N1B4_5645 0.35 -3.4
74,656 - Pf1N1B4_5645 0.43 +0.8
74,721 + Pf1N1B4_5645 0.51 +0.2
74,722 - Pf1N1B4_5645 0.51 -1.1
74,722 - Pf1N1B4_5645 0.51 +1.0
74,999 - Pf1N1B4_5645 0.84 -0.2
75,188 - Pf1N1B4_5646 0.38 -1.8
75,577 + Pf1N1B4_5647 0.22 -0.8
75,625 + Pf1N1B4_5647 0.33 -0.1
75,625 + Pf1N1B4_5647 0.33 -1.8
75,625 + Pf1N1B4_5647 0.33 +0.8
75,626 - Pf1N1B4_5647 0.33 -0.3
75,627 + Pf1N1B4_5647 0.33 +0.8
75,627 + Pf1N1B4_5647 0.33 +1.2
75,627 + Pf1N1B4_5647 0.33 +0.6
75,627 + Pf1N1B4_5647 0.33 -0.6
75,627 + Pf1N1B4_5647 0.33 -2.1
75,647 - Pf1N1B4_5647 0.38 +0.2
75,712 + Pf1N1B4_5647 0.52 +1.0
75,869 + Pf1N1B4_5647 0.87 -2.6
75,869 + Pf1N1B4_5647 0.87 -0.1
76,062 + -0.7
76,062 + -0.6

Or see this region's nucleotide sequence