Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5642 and Pf1N1B4_5643 overlap by 4 nucleotides Pf1N1B4_5643 and Pf1N1B4_5644 are separated by 9 nucleotides Pf1N1B4_5644 and Pf1N1B4_5645 are separated by 52 nucleotides Pf1N1B4_5645 and Pf1N1B4_5646 overlap by 6 nucleotides
Pf1N1B4_5642 - ABC-type polar amino acid transport system, ATPase component, at 72,116 to 72,907
_5642
Pf1N1B4_5643 - Amino acid ABC transporter, permease protein, at 72,904 to 73,566
_5643
Pf1N1B4_5644 - amino acid ABC transporter, permease protein, at 73,576 to 74,238
_5644
Pf1N1B4_5645 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 74,291 to 75,136
_5645
Pf1N1B4_5646 - hypothetical protein, at 75,131 to 75,280
_5646
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 72.584 kb on - strand, within Pf1N1B4_5642 at 72.956 kb on + strand at 72.956 kb on + strand at 73.332 kb on - strand, within Pf1N1B4_5643 at 73.332 kb on - strand, within Pf1N1B4_5643 at 73.352 kb on - strand, within Pf1N1B4_5643 at 73.562 kb on + strand at 73.563 kb on - strand at 73.563 kb on - strand at 73.646 kb on + strand, within Pf1N1B4_5644 at 73.647 kb on - strand, within Pf1N1B4_5644 at 73.647 kb on - strand, within Pf1N1B4_5644 at 73.713 kb on - strand, within Pf1N1B4_5644 at 73.715 kb on + strand, within Pf1N1B4_5644 at 73.716 kb on - strand, within Pf1N1B4_5644 at 73.716 kb on - strand, within Pf1N1B4_5644 at 73.829 kb on + strand, within Pf1N1B4_5644 at 73.829 kb on + strand, within Pf1N1B4_5644 at 73.829 kb on + strand, within Pf1N1B4_5644 at 73.830 kb on - strand, within Pf1N1B4_5644 at 73.940 kb on + strand, within Pf1N1B4_5644 at 73.991 kb on - strand, within Pf1N1B4_5644 at 74.009 kb on + strand, within Pf1N1B4_5644 at 74.010 kb on - strand, within Pf1N1B4_5644 at 74.060 kb on + strand, within Pf1N1B4_5644 at 74.060 kb on + strand at 74.060 kb on + strand, within Pf1N1B4_5644 at 74.231 kb on + strand at 74.232 kb on - strand at 74.232 kb on - strand at 74.248 kb on + strand at 74.248 kb on + strand at 74.249 kb on - strand at 74.249 kb on - strand at 74.367 kb on + strand at 74.461 kb on - strand, within Pf1N1B4_5645 at 74.461 kb on - strand at 74.523 kb on + strand, within Pf1N1B4_5645 at 74.524 kb on - strand at 74.584 kb on - strand, within Pf1N1B4_5645 at 74.656 kb on - strand, within Pf1N1B4_5645 at 74.721 kb on + strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.999 kb on - strand, within Pf1N1B4_5645 at 75.188 kb on - strand, within Pf1N1B4_5646
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 72,584 - Pf1N1B4_5642 0.59 -1.4 72,956 + -0.8 72,956 + +0.5 73,332 - Pf1N1B4_5643 0.65 -1.6 73,332 - Pf1N1B4_5643 0.65 +0.2 73,352 - Pf1N1B4_5643 0.68 -1.4 73,562 + -1.3 73,563 - -1.6 73,563 - +0.7 73,646 + Pf1N1B4_5644 0.11 -0.2 73,647 - Pf1N1B4_5644 0.11 +0.2 73,647 - Pf1N1B4_5644 0.11 +0.2 73,713 - Pf1N1B4_5644 0.21 +0.9 73,715 + Pf1N1B4_5644 0.21 +0.8 73,716 - Pf1N1B4_5644 0.21 +1.2 73,716 - Pf1N1B4_5644 0.21 +1.9 73,829 + Pf1N1B4_5644 0.38 +0.0 73,829 + Pf1N1B4_5644 0.38 +2.8 73,829 + Pf1N1B4_5644 0.38 +0.2 73,830 - Pf1N1B4_5644 0.38 +2.0 73,940 + Pf1N1B4_5644 0.55 -0.8 73,991 - Pf1N1B4_5644 0.63 -1.1 74,009 + Pf1N1B4_5644 0.65 -0.4 74,010 - Pf1N1B4_5644 0.65 -2.0 74,060 + Pf1N1B4_5644 0.73 +0.5 74,060 + -1.4 74,060 + Pf1N1B4_5644 0.73 +0.7 74,231 + +0.0 74,232 - -1.1 74,232 - -1.1 74,248 + +0.3 74,248 + -0.2 74,249 - +0.1 74,249 - -1.0 74,367 + +0.2 74,461 - Pf1N1B4_5645 0.20 -1.6 74,461 - -1.1 74,523 + Pf1N1B4_5645 0.27 -1.7 74,524 - -1.8 74,584 - Pf1N1B4_5645 0.35 -3.4 74,656 - Pf1N1B4_5645 0.43 +0.8 74,721 + Pf1N1B4_5645 0.51 +0.2 74,722 - Pf1N1B4_5645 0.51 -1.1 74,722 - Pf1N1B4_5645 0.51 +1.0 74,999 - Pf1N1B4_5645 0.84 -0.2 75,188 - Pf1N1B4_5646 0.38 -1.8
Or see this region's nucleotide sequence