Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5582 and Pf1N1B4_5583 are separated by 104 nucleotides Pf1N1B4_5583 and Pf1N1B4_5584 are separated by 11 nucleotides Pf1N1B4_5584 and Pf1N1B4_5585 are separated by 103 nucleotides
Pf1N1B4_5582 - Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25), at 2,599 to 5,022
_5582
Pf1N1B4_5583 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 5,127 to 5,732
_5583
Pf1N1B4_5584 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 5,744 to 6,463
_5584
Pf1N1B4_5585 - LysR family transcriptional regulator YdcI, at 6,567 to 7,496
_5585
Position (kb) 5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4.351 kb on + strand, within Pf1N1B4_5582 at 4.351 kb on + strand, within Pf1N1B4_5582 at 4.351 kb on + strand, within Pf1N1B4_5582 at 4.419 kb on + strand, within Pf1N1B4_5582 at 4.447 kb on - strand, within Pf1N1B4_5582 at 4.710 kb on + strand, within Pf1N1B4_5582 at 4.822 kb on + strand at 4.822 kb on + strand at 4.822 kb on + strand at 4.823 kb on - strand at 4.825 kb on + strand at 4.825 kb on + strand at 4.825 kb on + strand at 4.909 kb on + strand at 4.909 kb on + strand at 4.909 kb on + strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.936 kb on + strand at 4.937 kb on - strand at 4.937 kb on - strand at 4.937 kb on - strand at 4.987 kb on + strand at 4.987 kb on + strand at 4.988 kb on - strand at 5.166 kb on - strand at 5.166 kb on - strand at 5.166 kb on - strand at 5.166 kb on - strand at 5.170 kb on - strand at 5.170 kb on - strand at 5.170 kb on - strand at 5.563 kb on + strand at 5.564 kb on - strand, within Pf1N1B4_5583 at 5.564 kb on - strand, within Pf1N1B4_5583 at 5.564 kb on - strand, within Pf1N1B4_5583 at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.683 kb on + strand at 5.684 kb on - strand at 5.684 kb on - strand at 5.925 kb on + strand, within Pf1N1B4_5584 at 6.051 kb on + strand, within Pf1N1B4_5584 at 6.054 kb on + strand, within Pf1N1B4_5584 at 6.054 kb on + strand, within Pf1N1B4_5584 at 6.054 kb on + strand, within Pf1N1B4_5584 at 6.179 kb on - strand, within Pf1N1B4_5584 at 6.390 kb on + strand, within Pf1N1B4_5584 at 6.421 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 4,351 + Pf1N1B4_5582 0.72 -1.4 4,351 + Pf1N1B4_5582 0.72 +0.6 4,351 + Pf1N1B4_5582 0.72 -2.4 4,419 + Pf1N1B4_5582 0.75 +0.5 4,447 - Pf1N1B4_5582 0.76 -2.0 4,710 + Pf1N1B4_5582 0.87 +0.2 4,822 + -1.1 4,822 + +1.2 4,822 + -0.4 4,823 - +1.4 4,825 + -0.4 4,825 + -0.2 4,825 + +1.2 4,909 + -1.4 4,909 + -1.5 4,909 + -0.7 4,910 - +0.8 4,910 - +0.4 4,910 - -0.1 4,910 - +1.2 4,910 - +0.2 4,910 - +0.7 4,910 - +0.2 4,910 - +0.4 4,936 + -0.0 4,937 - -0.3 4,937 - -0.7 4,937 - -0.2 4,987 + -1.4 4,987 + +0.8 4,988 - -0.8 5,166 - +0.2 5,166 - -1.4 5,166 - +0.1 5,166 - +1.3 5,170 - +0.2 5,170 - +0.9 5,170 - -2.8 5,563 + -2.4 5,564 - Pf1N1B4_5583 0.72 -0.5 5,564 - Pf1N1B4_5583 0.72 -1.1 5,564 - Pf1N1B4_5583 0.72 -0.8 5,679 + +1.8 5,679 + +0.4 5,679 + -1.8 5,679 + +0.5 5,679 + -1.4 5,679 + -0.7 5,679 + +0.9 5,679 + +0.8 5,680 - +0.2 5,680 - +1.8 5,680 - +1.3 5,680 - +0.6 5,680 - +0.9 5,680 - -0.4 5,680 - +0.6 5,680 - -0.1 5,680 - +0.8 5,680 - -0.3 5,680 - -0.1 5,680 - +0.7 5,683 + -1.2 5,684 - -0.1 5,684 - -1.4 5,925 + Pf1N1B4_5584 0.25 -1.4 6,051 + Pf1N1B4_5584 0.43 -0.4 6,054 + Pf1N1B4_5584 0.43 -1.4 6,054 + Pf1N1B4_5584 0.43 +0.2 6,054 + Pf1N1B4_5584 0.43 +0.2 6,179 - Pf1N1B4_5584 0.60 -0.8 6,390 + Pf1N1B4_5584 0.90 +0.2 6,421 + +1.1
Or see this region's nucleotide sequence