Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotides Pf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides Pf1N1B4_5502 and Pf1N1B4_5503 are separated by 499 nucleotides
Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862
_5500
Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413
_5501
Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542
_5502
Pf1N1B4_5503 - hypothetical protein, at 253,042 to 253,266
_5503
Position (kb) 251
252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.737 kb on - strand, within Pf1N1B4_5500 at 250.757 kb on - strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.771 kb on - strand at 250.805 kb on - strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.870 kb on + strand at 250.870 kb on + strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.977 kb on - strand at 250.977 kb on - strand at 251.024 kb on + strand at 251.024 kb on + strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.068 kb on - strand at 251.260 kb on + strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.279 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.411 kb on + strand at 251.411 kb on + strand at 251.412 kb on - strand at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.534 kb on + strand, within Pf1N1B4_5502 at 251.590 kb on + strand at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.740 kb on - strand, within Pf1N1B4_5502 at 251.798 kb on + strand, within Pf1N1B4_5502 at 251.994 kb on + strand, within Pf1N1B4_5502 at 251.995 kb on - strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.115 kb on - strand, within Pf1N1B4_5502 at 252.143 kb on + strand, within Pf1N1B4_5502 at 252.144 kb on - strand, within Pf1N1B4_5502 at 252.144 kb on - strand at 252.144 kb on - strand, within Pf1N1B4_5502 at 252.179 kb on + strand, within Pf1N1B4_5502 at 252.180 kb on - strand, within Pf1N1B4_5502 at 252.185 kb on + strand, within Pf1N1B4_5502 at 252.239 kb on + strand, within Pf1N1B4_5502 at 252.274 kb on + strand, within Pf1N1B4_5502 at 252.275 kb on - strand, within Pf1N1B4_5502 at 252.382 kb on + strand, within Pf1N1B4_5502 at 252.383 kb on - strand, within Pf1N1B4_5502 at 252.419 kb on + strand, within Pf1N1B4_5502 at 252.420 kb on - strand, within Pf1N1B4_5502 at 252.420 kb on - strand, within Pf1N1B4_5502 at 252.429 kb on + strand at 252.429 kb on + strand at 252.429 kb on + strand at 252.430 kb on - strand at 252.430 kb on - strand at 252.435 kb on + strand at 252.435 kb on + strand at 252.435 kb on + strand at 252.436 kb on - strand at 252.553 kb on + strand at 252.553 kb on + strand at 252.553 kb on + strand at 252.553 kb on + strand at 252.554 kb on - strand at 252.554 kb on - strand at 252.589 kb on + strand at 252.589 kb on + strand at 252.590 kb on - strand at 252.590 kb on - strand at 252.590 kb on - strand at 252.590 kb on - strand at 252.652 kb on - strand at 252.661 kb on + strand at 252.732 kb on + strand at 252.732 kb on + strand at 252.732 kb on + strand at 252.807 kb on - strand at 252.821 kb on + strand at 252.836 kb on - strand at 252.902 kb on + strand at 252.936 kb on + strand at 252.936 kb on + strand at 252.936 kb on + strand at 252.944 kb on - strand at 252.946 kb on + strand at 252.993 kb on + strand at 252.993 kb on + strand at 252.994 kb on - strand at 252.995 kb on + strand at 252.995 kb on + strand at 252.995 kb on + strand at 252.995 kb on + strand at 252.996 kb on - strand at 252.996 kb on - strand at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.130 kb on - strand, within Pf1N1B4_5503 at 253.130 kb on - strand, within Pf1N1B4_5503 at 253.267 kb on - strand at 253.315 kb on - strand at 253.315 kb on - strand at 253.380 kb on - strand at 253.380 kb on - strand at 253.426 kb on + strand at 253.427 kb on - strand at 253.427 kb on - strand at 253.441 kb on - strand at 253.473 kb on + strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.477 kb on - strand at 253.477 kb on - strand at 253.492 kb on - strand at 253.494 kb on + strand at 253.495 kb on - strand at 253.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 250,429 + Pf1N1B4_5500 0.56 +1.5 250,429 + Pf1N1B4_5500 0.56 -0.6 250,430 - Pf1N1B4_5500 0.56 +0.4 250,430 - Pf1N1B4_5500 0.56 +0.9 250,430 - Pf1N1B4_5500 0.56 +0.3 250,430 - Pf1N1B4_5500 0.56 -0.0 250,689 - Pf1N1B4_5500 0.82 -0.1 250,689 - Pf1N1B4_5500 0.82 +0.4 250,737 - Pf1N1B4_5500 0.87 -0.9 250,757 - -0.9 250,758 + Pf1N1B4_5500 0.89 -1.3 250,758 + -0.3 250,758 + Pf1N1B4_5500 0.89 -0.3 250,758 + Pf1N1B4_5500 0.89 -1.6 250,759 - Pf1N1B4_5500 0.89 +1.7 250,759 - Pf1N1B4_5500 0.89 -0.3 250,759 - Pf1N1B4_5500 0.89 +0.4 250,759 - Pf1N1B4_5500 0.89 +2.1 250,759 - Pf1N1B4_5500 0.89 -0.4 250,759 - Pf1N1B4_5500 0.89 +0.1 250,759 - Pf1N1B4_5500 0.89 -0.4 250,759 - Pf1N1B4_5500 0.89 -0.7 250,771 - -1.6 250,805 - +1.6 250,807 + -2.1 250,807 + -1.9 250,807 + -1.3 250,870 + +1.4 250,870 + -0.3 250,871 - +0.1 250,871 - -1.3 250,871 - -1.0 250,976 + +1.1 250,976 + -0.5 250,976 + +0.7 250,977 - -0.6 250,977 - +0.5 251,024 + +1.0 251,024 + +1.7 251,025 - +0.7 251,025 - -0.3 251,025 - +0.6 251,025 - -1.1 251,025 - -0.3 251,025 - -0.3 251,067 + -0.3 251,067 + -1.3 251,067 + -0.3 251,068 - +0.7 251,260 + Pf1N1B4_5501 0.55 +0.4 251,261 - Pf1N1B4_5501 0.55 +0.1 251,261 - Pf1N1B4_5501 0.55 -0.9 251,279 + Pf1N1B4_5501 0.61 -0.0 251,302 + Pf1N1B4_5501 0.67 -1.1 251,302 + Pf1N1B4_5501 0.67 +0.1 251,302 + Pf1N1B4_5501 0.67 -0.2 251,302 + Pf1N1B4_5501 0.67 +0.7 251,303 - Pf1N1B4_5501 0.68 -0.3 251,303 - Pf1N1B4_5501 0.68 -0.3 251,303 - Pf1N1B4_5501 0.68 -0.9 251,411 + +0.7 251,411 + -0.3 251,412 - +0.2 251,517 + Pf1N1B4_5502 0.12 +0.3 251,517 + Pf1N1B4_5502 0.12 -0.3 251,517 + Pf1N1B4_5502 0.12 +1.3 251,517 + Pf1N1B4_5502 0.12 -0.6 251,517 + Pf1N1B4_5502 0.12 +0.5 251,517 + Pf1N1B4_5502 0.12 +1.1 251,518 - Pf1N1B4_5502 0.12 +1.7 251,518 - Pf1N1B4_5502 0.12 -0.4 251,534 + Pf1N1B4_5502 0.13 -0.5 251,590 + -0.6 251,739 + Pf1N1B4_5502 0.31 -0.9 251,739 + Pf1N1B4_5502 0.31 -0.3 251,739 + Pf1N1B4_5502 0.31 +0.9 251,740 - Pf1N1B4_5502 0.31 +0.7 251,798 + Pf1N1B4_5502 0.36 -0.9 251,994 + Pf1N1B4_5502 0.53 -0.3 251,995 - Pf1N1B4_5502 0.53 -0.9 252,043 + Pf1N1B4_5502 0.57 -0.3 252,043 + Pf1N1B4_5502 0.57 +0.2 252,043 + Pf1N1B4_5502 0.57 +0.7 252,043 + Pf1N1B4_5502 0.57 +1.3 252,043 + Pf1N1B4_5502 0.57 -1.6 252,044 - Pf1N1B4_5502 0.57 -0.1 252,044 - Pf1N1B4_5502 0.57 +1.6 252,044 - +0.7 252,044 - Pf1N1B4_5502 0.57 -1.7 252,044 - Pf1N1B4_5502 0.57 -0.6 252,044 - Pf1N1B4_5502 0.57 +1.0 252,044 - Pf1N1B4_5502 0.57 -0.9 252,044 - Pf1N1B4_5502 0.57 -0.3 252,044 - Pf1N1B4_5502 0.57 +1.5 252,115 - Pf1N1B4_5502 0.63 +0.7 252,143 + Pf1N1B4_5502 0.66 +0.5 252,144 - Pf1N1B4_5502 0.66 -0.7 252,144 - -0.9 252,144 - Pf1N1B4_5502 0.66 +1.0 252,179 + Pf1N1B4_5502 0.69 +0.9 252,180 - Pf1N1B4_5502 0.69 -0.4 252,185 + Pf1N1B4_5502 0.69 +1.0 252,239 + Pf1N1B4_5502 0.74 +1.0 252,274 + Pf1N1B4_5502 0.77 -0.0 252,275 - Pf1N1B4_5502 0.77 +0.7 252,382 + Pf1N1B4_5502 0.86 +0.5 252,383 - Pf1N1B4_5502 0.86 +1.5 252,419 + Pf1N1B4_5502 0.89 -0.2 252,420 - Pf1N1B4_5502 0.89 -0.1 252,420 - Pf1N1B4_5502 0.89 -0.2 252,429 + +0.1 252,429 + +0.1 252,429 + -2.4 252,430 - -1.4 252,430 - -1.5 252,435 + -0.9 252,435 + -0.1 252,435 + -1.1 252,436 - +0.1 252,553 + +1.1 252,553 + +0.1 252,553 + -0.9 252,553 + -1.6 252,554 - +0.3 252,554 - +0.2 252,589 + -1.1 252,589 + +0.7 252,590 - +0.1 252,590 - -2.6 252,590 - +1.5 252,590 - +0.9 252,652 - +0.7 252,661 + +1.1 252,732 + +0.4 252,732 + -3.1 252,732 + -2.6 252,807 - -0.3 252,821 + +0.5 252,836 - -1.3 252,902 + -1.6 252,936 + -3.0 252,936 + -0.7 252,936 + -1.1 252,944 - -0.9 252,946 + -2.4 252,993 + -1.3 252,993 + -3.1 252,994 - -0.6 252,995 + -3.2 252,995 + -2.9 252,995 + +0.1 252,995 + -2.1 252,996 - -0.9 252,996 - -2.7 253,123 + Pf1N1B4_5503 0.36 +0.3 253,123 + Pf1N1B4_5503 0.36 +1.4 253,123 + Pf1N1B4_5503 0.36 +1.4 253,123 + Pf1N1B4_5503 0.36 +1.2 253,123 + Pf1N1B4_5503 0.36 +1.6 253,124 - Pf1N1B4_5503 0.36 -1.3 253,124 - Pf1N1B4_5503 0.36 -0.5 253,124 - Pf1N1B4_5503 0.36 -0.3 253,124 - Pf1N1B4_5503 0.36 -2.3 253,124 - Pf1N1B4_5503 0.36 +0.3 253,124 - Pf1N1B4_5503 0.36 +0.1 253,124 - Pf1N1B4_5503 0.36 +1.3 253,124 - Pf1N1B4_5503 0.36 -1.1 253,124 - Pf1N1B4_5503 0.36 -1.6 253,129 + Pf1N1B4_5503 0.39 +0.8 253,129 + Pf1N1B4_5503 0.39 +0.1 253,129 + Pf1N1B4_5503 0.39 -0.3 253,129 + Pf1N1B4_5503 0.39 +1.0 253,129 + Pf1N1B4_5503 0.39 -0.6 253,130 - Pf1N1B4_5503 0.39 -0.4 253,130 - Pf1N1B4_5503 0.39 -1.1 253,267 - +0.3 253,315 - +0.1 253,315 - +0.3 253,380 - -0.0 253,380 - -0.6 253,426 + -0.7 253,427 - -0.3 253,427 - -0.6 253,441 - +0.1 253,473 + +0.1 253,474 - -1.3 253,474 - +1.1 253,474 - -0.4 253,474 - -0.8 253,474 - -0.1 253,474 - +0.7 253,477 - -2.4 253,477 - +0.3 253,492 - -2.1 253,494 + -1.6 253,495 - +0.5 253,518 + +1.5
Or see this region's nucleotide sequence