Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5438 and Pf1N1B4_5439 are separated by 829 nucleotides Pf1N1B4_5439 and Pf1N1B4_5440 are separated by 715 nucleotides
Pf1N1B4_5438 - hypothetical protein, at 183,761 to 184,024
_5438
Pf1N1B4_5439 - hypothetical protein, at 184,854 to 185,147
_5439
Pf1N1B4_5440 - hypothetical protein, at 185,863 to 185,985
_5440
Position (kb)
184
185
186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.927 kb on + strand, within Pf1N1B4_5438 at 183.928 kb on - strand, within Pf1N1B4_5438 at 183.995 kb on + strand, within Pf1N1B4_5438 at 183.995 kb on + strand, within Pf1N1B4_5438 at 183.995 kb on + strand, within Pf1N1B4_5438 at 183.996 kb on - strand, within Pf1N1B4_5438 at 183.996 kb on - strand, within Pf1N1B4_5438 at 183.996 kb on - strand, within Pf1N1B4_5438 at 184.119 kb on - strand at 184.119 kb on - strand at 184.161 kb on + strand at 184.162 kb on - strand at 184.162 kb on - strand at 184.162 kb on - strand at 184.174 kb on + strand at 184.340 kb on + strand at 184.340 kb on + strand at 184.341 kb on - strand at 184.341 kb on - strand at 184.357 kb on + strand at 184.357 kb on + strand at 184.358 kb on - strand at 184.506 kb on + strand at 184.507 kb on - strand at 184.507 kb on - strand at 184.557 kb on + strand at 184.558 kb on - strand at 184.558 kb on - strand at 184.558 kb on - strand at 184.679 kb on + strand at 184.719 kb on - strand at 184.719 kb on - strand at 184.739 kb on + strand at 184.739 kb on + strand at 184.740 kb on - strand at 184.889 kb on + strand, within Pf1N1B4_5439 at 184.918 kb on + strand, within Pf1N1B4_5439 at 184.918 kb on + strand, within Pf1N1B4_5439 at 185.006 kb on - strand, within Pf1N1B4_5439 at 185.006 kb on - strand, within Pf1N1B4_5439 at 185.019 kb on - strand, within Pf1N1B4_5439 at 185.076 kb on - strand, within Pf1N1B4_5439 at 185.144 kb on - strand at 185.144 kb on - strand at 185.144 kb on - strand at 185.144 kb on - strand at 185.168 kb on + strand at 185.168 kb on + strand at 185.168 kb on + strand at 185.169 kb on - strand at 185.223 kb on - strand at 185.223 kb on - strand at 185.223 kb on - strand at 185.234 kb on + strand at 185.235 kb on - strand at 185.249 kb on - strand at 185.249 kb on - strand at 185.252 kb on + strand at 185.252 kb on + strand at 185.252 kb on + strand at 185.253 kb on - strand at 185.253 kb on - strand at 185.291 kb on + strand at 185.292 kb on - strand at 185.326 kb on + strand at 185.327 kb on - strand at 185.327 kb on - strand at 185.422 kb on + strand at 185.422 kb on + strand at 185.422 kb on + strand at 185.422 kb on + strand at 185.423 kb on - strand at 185.423 kb on - strand at 185.423 kb on - strand at 185.423 kb on - strand at 185.427 kb on - strand at 185.427 kb on - strand at 185.454 kb on - strand at 185.454 kb on - strand at 185.491 kb on + strand at 185.491 kb on + strand at 185.491 kb on + strand at 185.512 kb on + strand at 185.517 kb on + strand at 185.517 kb on + strand at 185.517 kb on + strand at 185.517 kb on + strand at 185.517 kb on + strand at 185.517 kb on + strand at 185.517 kb on + strand at 185.518 kb on - strand at 185.518 kb on - strand at 185.518 kb on - strand at 185.518 kb on - strand at 185.518 kb on - strand at 185.518 kb on - strand at 185.518 kb on - strand at 185.523 kb on + strand at 185.523 kb on + strand at 185.523 kb on + strand at 185.524 kb on - strand at 185.524 kb on - strand at 185.524 kb on - strand at 185.524 kb on - strand at 185.524 kb on - strand at 185.683 kb on - strand at 185.729 kb on + strand at 185.729 kb on + strand at 185.729 kb on + strand at 185.777 kb on + strand at 185.777 kb on + strand at 185.777 kb on + strand at 185.777 kb on + strand at 185.777 kb on + strand at 185.778 kb on - strand at 185.778 kb on - strand at 185.778 kb on - strand at 185.778 kb on - strand at 185.814 kb on - strand at 186.017 kb on + strand at 186.017 kb on + strand at 186.018 kb on - strand at 186.018 kb on - strand at 186.042 kb on - strand at 186.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 183,927 + Pf1N1B4_5438 0.63 -0.0 183,927 + Pf1N1B4_5438 0.63 -2.1 183,927 + Pf1N1B4_5438 0.63 -0.0 183,927 + Pf1N1B4_5438 0.63 +0.3 183,927 + Pf1N1B4_5438 0.63 +1.0 183,927 + Pf1N1B4_5438 0.63 +0.8 183,927 + Pf1N1B4_5438 0.63 +1.7 183,928 - Pf1N1B4_5438 0.63 -1.4 183,995 + Pf1N1B4_5438 0.89 +0.7 183,995 + Pf1N1B4_5438 0.89 -1.2 183,995 + Pf1N1B4_5438 0.89 -1.9 183,996 - Pf1N1B4_5438 0.89 -1.1 183,996 - Pf1N1B4_5438 0.89 +1.3 183,996 - Pf1N1B4_5438 0.89 +1.2 184,119 - +0.5 184,119 - +0.1 184,161 + +0.8 184,162 - +0.6 184,162 - -1.4 184,162 - +0.7 184,174 + +1.5 184,340 + +0.7 184,340 + +1.1 184,341 - -0.6 184,341 - +0.1 184,357 + -0.7 184,357 + -0.5 184,358 - -0.3 184,506 + +0.6 184,507 - +0.1 184,507 - +0.7 184,557 + -0.9 184,558 - -1.7 184,558 - -0.2 184,558 - -1.5 184,679 + +0.7 184,719 - -0.3 184,719 - -0.7 184,739 + -0.4 184,739 + +0.5 184,740 - -1.4 184,889 + Pf1N1B4_5439 0.12 -1.0 184,918 + Pf1N1B4_5439 0.22 -0.6 184,918 + Pf1N1B4_5439 0.22 -0.3 185,006 - Pf1N1B4_5439 0.52 -1.9 185,006 - Pf1N1B4_5439 0.52 +0.4 185,019 - Pf1N1B4_5439 0.56 -1.9 185,076 - Pf1N1B4_5439 0.76 -2.2 185,144 - +0.7 185,144 - +1.1 185,144 - +1.6 185,144 - -0.5 185,168 + -0.3 185,168 + +0.7 185,168 + -0.0 185,169 - -1.6 185,223 - +0.3 185,223 - +0.2 185,223 - +1.1 185,234 + +0.1 185,235 - -0.6 185,249 - -0.5 185,249 - -0.3 185,252 + +0.5 185,252 + +0.9 185,252 + +1.3 185,253 - -0.6 185,253 - +1.5 185,291 + +0.7 185,292 - -1.3 185,326 + +0.1 185,327 - -0.3 185,327 - -0.7 185,422 + +0.7 185,422 + -0.6 185,422 + -0.3 185,422 + -0.1 185,423 - +0.6 185,423 - -2.3 185,423 - -0.8 185,423 - -0.7 185,427 - +1.1 185,427 - -1.3 185,454 - +0.3 185,454 - +0.7 185,491 + -1.3 185,491 + -1.6 185,491 + +0.1 185,512 + +0.1 185,517 + -2.9 185,517 + +1.7 185,517 + +1.0 185,517 + -1.1 185,517 + -0.0 185,517 + +0.3 185,517 + +0.0 185,518 - -0.6 185,518 - -0.3 185,518 - +1.1 185,518 - -0.9 185,518 - -0.5 185,518 - -0.1 185,518 - -1.6 185,523 + +1.0 185,523 + -0.4 185,523 + -0.6 185,524 - -0.6 185,524 - +0.5 185,524 - +0.1 185,524 - -1.4 185,524 - +0.3 185,683 - +0.2 185,729 + -0.0 185,729 + +0.9 185,729 + -1.6 185,777 + -0.6 185,777 + +0.7 185,777 + -0.1 185,777 + +0.1 185,777 + +1.7 185,778 - -0.3 185,778 - +0.1 185,778 - +0.1 185,778 - -0.3 185,814 - -0.6 186,017 + +0.5 186,017 + +1.3 186,018 - +1.7 186,018 - +1.8 186,042 - -1.9 186,042 - -0.1
Or see this region's nucleotide sequence