Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4999 and Pf1N1B4_5000 are separated by 169 nucleotides Pf1N1B4_5000 and Pf1N1B4_5001 are separated by 331 nucleotides
Pf1N1B4_4999 - Sugar diacid utilization regulator SdaR, at 5,548,023 to 5,549,153
_4999
Pf1N1B4_5000 - D-glycerate transporter (predicted), at 5,549,323 to 5,550,633
_5000
Pf1N1B4_5001 - Oxidoreductase, aldo/keto reductase family, at 5,550,965 to 5,551,978
_5001
Position (kb) 5549
5550
5551 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5548.470 kb on - strand, within Pf1N1B4_4999 at 5548.849 kb on + strand, within Pf1N1B4_4999 at 5548.849 kb on + strand at 5548.849 kb on + strand, within Pf1N1B4_4999 at 5548.849 kb on + strand at 5548.850 kb on - strand, within Pf1N1B4_4999 at 5549.304 kb on - strand at 5549.304 kb on - strand at 5549.434 kb on + strand at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.544 kb on - strand, within Pf1N1B4_5000 at 5549.544 kb on - strand, within Pf1N1B4_5000 at 5549.935 kb on + strand, within Pf1N1B4_5000 at 5549.956 kb on + strand, within Pf1N1B4_5000 at 5550.234 kb on + strand, within Pf1N1B4_5000 at 5550.234 kb on + strand, within Pf1N1B4_5000 at 5550.235 kb on - strand at 5550.235 kb on - strand, within Pf1N1B4_5000 at 5550.235 kb on - strand, within Pf1N1B4_5000 at 5550.563 kb on - strand at 5550.563 kb on - strand at 5550.632 kb on - strand at 5550.632 kb on - strand at 5550.672 kb on + strand at 5550.687 kb on + strand at 5550.687 kb on + strand at 5550.687 kb on + strand at 5550.688 kb on - strand at 5550.688 kb on - strand at 5550.688 kb on - strand at 5550.688 kb on - strand at 5550.696 kb on + strand at 5550.925 kb on + strand at 5550.926 kb on - strand at 5551.222 kb on + strand, within Pf1N1B4_5001 at 5551.313 kb on + strand, within Pf1N1B4_5001 at 5551.313 kb on + strand, within Pf1N1B4_5001 at 5551.313 kb on + strand, within Pf1N1B4_5001 at 5551.314 kb on - strand, within Pf1N1B4_5001 at 5551.314 kb on - strand, within Pf1N1B4_5001 at 5551.316 kb on + strand, within Pf1N1B4_5001 at 5551.317 kb on - strand, within Pf1N1B4_5001 at 5551.499 kb on + strand, within Pf1N1B4_5001 at 5551.500 kb on - strand, within Pf1N1B4_5001 at 5551.575 kb on - strand, within Pf1N1B4_5001 at 5551.610 kb on + strand, within Pf1N1B4_5001
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,548,470 - Pf1N1B4_4999 0.40 +0.4 5,548,849 + Pf1N1B4_4999 0.73 -0.8 5,548,849 + +1.8 5,548,849 + Pf1N1B4_4999 0.73 +0.3 5,548,849 + -1.0 5,548,850 - Pf1N1B4_4999 0.73 -0.5 5,549,304 - +1.0 5,549,304 - -0.8 5,549,434 + -0.7 5,549,543 + Pf1N1B4_5000 0.17 -1.0 5,549,543 + Pf1N1B4_5000 0.17 +0.3 5,549,543 + Pf1N1B4_5000 0.17 -1.8 5,549,543 + Pf1N1B4_5000 0.17 -2.0 5,549,544 - Pf1N1B4_5000 0.17 -1.6 5,549,544 - Pf1N1B4_5000 0.17 +1.0 5,549,935 + Pf1N1B4_5000 0.47 -2.0 5,549,956 + Pf1N1B4_5000 0.48 -1.8 5,550,234 + Pf1N1B4_5000 0.69 -1.0 5,550,234 + Pf1N1B4_5000 0.69 -0.0 5,550,235 - +3.0 5,550,235 - Pf1N1B4_5000 0.70 -2.6 5,550,235 - Pf1N1B4_5000 0.70 -2.0 5,550,563 - -2.8 5,550,563 - +0.2 5,550,632 - -2.8 5,550,632 - +0.5 5,550,672 + +0.1 5,550,687 + +1.2 5,550,687 + +0.4 5,550,687 + +0.5 5,550,688 - +0.9 5,550,688 - +0.3 5,550,688 - -2.6 5,550,688 - -1.6 5,550,696 + -0.0 5,550,925 + -1.0 5,550,926 - -1.7 5,551,222 + Pf1N1B4_5001 0.25 +0.8 5,551,313 + Pf1N1B4_5001 0.34 -0.4 5,551,313 + Pf1N1B4_5001 0.34 +0.4 5,551,313 + Pf1N1B4_5001 0.34 +1.0 5,551,314 - Pf1N1B4_5001 0.34 +2.0 5,551,314 - Pf1N1B4_5001 0.34 -0.2 5,551,316 + Pf1N1B4_5001 0.35 +0.1 5,551,317 - Pf1N1B4_5001 0.35 +1.0 5,551,499 + Pf1N1B4_5001 0.53 +0.4 5,551,500 - Pf1N1B4_5001 0.53 +0.1 5,551,575 - Pf1N1B4_5001 0.60 +1.8 5,551,610 + Pf1N1B4_5001 0.64 -2.0
Or see this region's nucleotide sequence