Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4943 and Pf1N1B4_4944 are separated by 6 nucleotides Pf1N1B4_4944 and Pf1N1B4_4945 are separated by 114 nucleotides Pf1N1B4_4945 and Pf1N1B4_4946 are separated by 78 nucleotides Pf1N1B4_4946 and Pf1N1B4_4947 are separated by 306 nucleotides
Pf1N1B4_4943 - Oxidoreductase, short chain dehydrogenase/reductase family, at 5,479,228 to 5,480,019
_4943
Pf1N1B4_4944 - hypothetical protein, at 5,480,026 to 5,480,151
_4944
Pf1N1B4_4945 - hypothetical protein, at 5,480,266 to 5,480,439
_4945
Pf1N1B4_4946 - putative aminotransferase, at 5,480,518 to 5,481,672
_4946
Pf1N1B4_4947 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 5,481,979 to 5,485,044
_4947
Position (kb)
5480
5481
5482 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5479.985 kb on + strand at 5480.206 kb on + strand at 5480.206 kb on + strand at 5480.206 kb on + strand at 5480.224 kb on + strand at 5480.225 kb on - strand at 5480.282 kb on + strand at 5480.283 kb on - strand at 5480.332 kb on + strand, within Pf1N1B4_4945 at 5480.332 kb on + strand, within Pf1N1B4_4945 at 5480.333 kb on - strand, within Pf1N1B4_4945 at 5480.333 kb on - strand at 5480.469 kb on + strand at 5480.541 kb on + strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.723 kb on + strand, within Pf1N1B4_4946 at 5480.978 kb on + strand, within Pf1N1B4_4946 at 5480.979 kb on - strand, within Pf1N1B4_4946 at 5480.979 kb on - strand, within Pf1N1B4_4946 at 5481.052 kb on + strand, within Pf1N1B4_4946 at 5481.053 kb on - strand, within Pf1N1B4_4946 at 5481.053 kb on - strand, within Pf1N1B4_4946 at 5481.245 kb on + strand at 5481.246 kb on - strand, within Pf1N1B4_4946 at 5481.446 kb on + strand, within Pf1N1B4_4946 at 5481.901 kb on + strand at 5482.165 kb on + strand at 5482.166 kb on - strand at 5482.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,479,985 + +0.4 5,480,206 + -1.9 5,480,206 + -0.3 5,480,206 + -0.0 5,480,224 + -1.0 5,480,225 - +2.3 5,480,282 + -0.5 5,480,283 - +0.8 5,480,332 + Pf1N1B4_4945 0.38 -1.6 5,480,332 + Pf1N1B4_4945 0.38 -1.8 5,480,333 - Pf1N1B4_4945 0.39 -1.2 5,480,333 - +1.0 5,480,469 + -2.2 5,480,541 + +1.0 5,480,542 - -1.0 5,480,542 - -1.0 5,480,542 - -1.0 5,480,542 - +1.3 5,480,542 - -1.0 5,480,723 + Pf1N1B4_4946 0.18 -1.2 5,480,978 + Pf1N1B4_4946 0.40 -0.2 5,480,979 - Pf1N1B4_4946 0.40 -0.0 5,480,979 - Pf1N1B4_4946 0.40 -1.6 5,481,052 + Pf1N1B4_4946 0.46 +0.4 5,481,053 - Pf1N1B4_4946 0.46 -1.0 5,481,053 - Pf1N1B4_4946 0.46 -2.2 5,481,245 + +1.0 5,481,246 - Pf1N1B4_4946 0.63 -1.6 5,481,446 + Pf1N1B4_4946 0.80 -1.2 5,481,901 + +0.6 5,482,165 + -0.0 5,482,166 - +0.5 5,482,166 - -0.3
Or see this region's nucleotide sequence