Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4902 and Pf1N1B4_4903 are separated by 14 nucleotides Pf1N1B4_4903 and Pf1N1B4_4904 are separated by 210 nucleotides
Pf1N1B4_4902 - ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE, at 5,425,176 to 5,426,525
_4902
Pf1N1B4_4903 - ABC-type protease exporter, outer membrane component PrtF/AprF, at 5,426,540 to 5,427,892
_4903
Pf1N1B4_4904 - Serine protease homologue, at 5,428,103 to 5,431,066
_4904
Position (kb)
5426
5427
5428 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5425.831 kb on - strand, within Pf1N1B4_4902 at 5425.974 kb on + strand, within Pf1N1B4_4902 at 5426.472 kb on + strand at 5426.540 kb on - strand at 5426.540 kb on - strand at 5426.545 kb on + strand at 5426.545 kb on + strand at 5426.546 kb on - strand at 5426.739 kb on - strand, within Pf1N1B4_4903 at 5426.776 kb on + strand, within Pf1N1B4_4903 at 5426.966 kb on + strand at 5426.967 kb on - strand at 5427.111 kb on - strand, within Pf1N1B4_4903 at 5427.266 kb on + strand, within Pf1N1B4_4903 at 5427.435 kb on - strand, within Pf1N1B4_4903 at 5427.435 kb on - strand, within Pf1N1B4_4903 at 5427.629 kb on - strand, within Pf1N1B4_4903 at 5427.629 kb on - strand, within Pf1N1B4_4903 at 5427.629 kb on - strand, within Pf1N1B4_4903 at 5427.629 kb on - strand, within Pf1N1B4_4903 at 5427.815 kb on + strand at 5427.816 kb on - strand at 5427.816 kb on - strand at 5427.816 kb on - strand at 5427.816 kb on - strand at 5427.819 kb on - strand at 5427.819 kb on - strand at 5428.070 kb on + strand at 5428.070 kb on + strand at 5428.071 kb on - strand at 5428.071 kb on - strand at 5428.071 kb on - strand at 5428.116 kb on + strand at 5428.117 kb on - strand at 5428.117 kb on - strand at 5428.147 kb on - strand at 5428.190 kb on + strand at 5428.287 kb on - strand at 5428.287 kb on - strand at 5428.287 kb on - strand at 5428.404 kb on - strand, within Pf1N1B4_4904 at 5428.404 kb on - strand, within Pf1N1B4_4904 at 5428.404 kb on - strand, within Pf1N1B4_4904 at 5428.527 kb on - strand, within Pf1N1B4_4904 at 5428.542 kb on - strand, within Pf1N1B4_4904 at 5428.568 kb on - strand, within Pf1N1B4_4904 at 5428.616 kb on + strand at 5428.617 kb on - strand, within Pf1N1B4_4904 at 5428.847 kb on + strand, within Pf1N1B4_4904
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,425,831 - Pf1N1B4_4902 0.49 -1.6 5,425,974 + Pf1N1B4_4902 0.59 +0.4 5,426,472 + +1.0 5,426,540 - -1.0 5,426,540 - -0.6 5,426,545 + +1.2 5,426,545 + -1.4 5,426,546 - -0.3 5,426,739 - Pf1N1B4_4903 0.15 -1.6 5,426,776 + Pf1N1B4_4903 0.17 +0.4 5,426,966 + -0.0 5,426,967 - -1.0 5,427,111 - Pf1N1B4_4903 0.42 +0.4 5,427,266 + Pf1N1B4_4903 0.54 -1.5 5,427,435 - Pf1N1B4_4903 0.66 -1.2 5,427,435 - Pf1N1B4_4903 0.66 -0.7 5,427,629 - Pf1N1B4_4903 0.80 -0.0 5,427,629 - Pf1N1B4_4903 0.80 -1.3 5,427,629 - Pf1N1B4_4903 0.80 +0.4 5,427,629 - Pf1N1B4_4903 0.80 -0.8 5,427,815 + +1.0 5,427,816 - +0.8 5,427,816 - -0.6 5,427,816 - -0.4 5,427,816 - -0.5 5,427,819 - +0.8 5,427,819 - +0.4 5,428,070 + +0.5 5,428,070 + -0.0 5,428,071 - +2.3 5,428,071 - +0.0 5,428,071 - +0.8 5,428,116 + -0.5 5,428,117 - +1.4 5,428,117 - -0.8 5,428,147 - -1.6 5,428,190 + -0.3 5,428,287 - -0.0 5,428,287 - -2.4 5,428,287 - +0.4 5,428,404 - Pf1N1B4_4904 0.10 -2.0 5,428,404 - Pf1N1B4_4904 0.10 -0.9 5,428,404 - Pf1N1B4_4904 0.10 +0.8 5,428,527 - Pf1N1B4_4904 0.14 -1.0 5,428,542 - Pf1N1B4_4904 0.15 +0.2 5,428,568 - Pf1N1B4_4904 0.16 +0.5 5,428,616 + -0.0 5,428,617 - Pf1N1B4_4904 0.17 -1.0 5,428,847 + Pf1N1B4_4904 0.25 -0.6
Or see this region's nucleotide sequence