Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4780 and Pf1N1B4_4781 are separated by 56 nucleotides Pf1N1B4_4781 and Pf1N1B4_4782 are separated by 160 nucleotides Pf1N1B4_4782 and Pf1N1B4_4783 are separated by 157 nucleotides
Pf1N1B4_4780 - COGs COG3558, at 5,281,745 to 5,282,221
_4780
Pf1N1B4_4781 - Transcriptional regulator, TetR family, at 5,282,278 to 5,282,898
_4781
Pf1N1B4_4782 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 5,283,059 to 5,284,402
_4782
Pf1N1B4_4783 - Transcriptional regulator, AraC family, at 5,284,560 to 5,285,576
_4783
Position (kb) 5283
5284
5285 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5282.216 kb on - strand at 5282.222 kb on - strand at 5282.222 kb on - strand at 5282.224 kb on - strand at 5282.243 kb on - strand at 5282.267 kb on + strand at 5282.710 kb on - strand, within Pf1N1B4_4781 at 5282.991 kb on - strand at 5283.017 kb on + strand at 5283.017 kb on + strand at 5283.018 kb on - strand at 5283.059 kb on - strand at 5283.059 kb on - strand at 5283.091 kb on + strand at 5283.092 kb on - strand at 5283.200 kb on + strand at 5283.200 kb on + strand, within Pf1N1B4_4782 at 5283.200 kb on + strand at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.216 kb on - strand, within Pf1N1B4_4782 at 5283.722 kb on + strand, within Pf1N1B4_4782 at 5283.723 kb on - strand, within Pf1N1B4_4782 at 5284.010 kb on + strand, within Pf1N1B4_4782 at 5284.112 kb on + strand, within Pf1N1B4_4782 at 5284.113 kb on - strand, within Pf1N1B4_4782 at 5284.113 kb on - strand, within Pf1N1B4_4782 at 5284.475 kb on + strand at 5284.519 kb on + strand at 5284.519 kb on + strand at 5284.520 kb on - strand at 5284.714 kb on - strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.975 kb on - strand at 5284.984 kb on - strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,282,216 - +0.6 5,282,222 - +0.3 5,282,222 - +0.4 5,282,224 - -0.6 5,282,243 - +0.7 5,282,267 + -2.6 5,282,710 - Pf1N1B4_4781 0.70 -2.0 5,282,991 - -1.0 5,283,017 + -0.7 5,283,017 + -0.3 5,283,018 - -2.3 5,283,059 - -2.6 5,283,059 - +0.6 5,283,091 + -0.2 5,283,092 - -0.3 5,283,200 + +1.0 5,283,200 + Pf1N1B4_4782 0.10 -1.3 5,283,200 + +1.2 5,283,201 - Pf1N1B4_4782 0.11 -1.3 5,283,201 - Pf1N1B4_4782 0.11 -1.0 5,283,201 - Pf1N1B4_4782 0.11 -2.8 5,283,201 - Pf1N1B4_4782 0.11 -0.4 5,283,201 - Pf1N1B4_4782 0.11 -1.2 5,283,201 - -1.6 5,283,201 - Pf1N1B4_4782 0.11 -2.3 5,283,216 - Pf1N1B4_4782 0.12 +1.0 5,283,722 + Pf1N1B4_4782 0.49 -1.2 5,283,723 - Pf1N1B4_4782 0.49 +0.0 5,284,010 + Pf1N1B4_4782 0.71 -1.0 5,284,112 + Pf1N1B4_4782 0.78 +0.0 5,284,113 - Pf1N1B4_4782 0.78 -1.3 5,284,113 - Pf1N1B4_4782 0.78 +0.2 5,284,475 + -0.2 5,284,519 + +1.2 5,284,519 + -0.8 5,284,520 - -1.0 5,284,714 - Pf1N1B4_4783 0.15 +1.0 5,284,974 + Pf1N1B4_4783 0.41 -0.6 5,284,974 + Pf1N1B4_4783 0.41 -0.2 5,284,975 - -1.0 5,284,984 - Pf1N1B4_4783 0.42 -0.5 5,285,385 + Pf1N1B4_4783 0.81 +0.1 5,285,385 + Pf1N1B4_4783 0.81 -0.7 5,285,385 + Pf1N1B4_4783 0.81 -0.0
Or see this region's nucleotide sequence