Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4732 and Pf1N1B4_4733 are separated by 157 nucleotides Pf1N1B4_4733 and Pf1N1B4_4734 are separated by 32 nucleotides Pf1N1B4_4734 and Pf1N1B4_4735 are separated by 62 nucleotides
Pf1N1B4_4732 - Fap amyloid fiber secretin, at 5,229,825 to 5,230,994
_4732
Pf1N1B4_4733 - Fap system putative outer membrane protein, at 5,231,152 to 5,231,889
_4733
Pf1N1B4_4734 - Fap protein with C39 domain, at 5,231,922 to 5,232,602
_4734
Pf1N1B4_4735 - Fap amyloid fibril major component, at 5,232,665 to 5,233,954
_4735
Position (kb)
5231
5232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5230.177 kb on + strand at 5230.177 kb on + strand at 5230.177 kb on + strand, within Pf1N1B4_4732 at 5230.183 kb on + strand, within Pf1N1B4_4732 at 5230.184 kb on - strand at 5230.184 kb on - strand, within Pf1N1B4_4732 at 5230.411 kb on + strand, within Pf1N1B4_4732 at 5230.507 kb on + strand, within Pf1N1B4_4732 at 5230.507 kb on + strand at 5230.687 kb on + strand, within Pf1N1B4_4732 at 5230.688 kb on - strand, within Pf1N1B4_4732 at 5230.696 kb on + strand, within Pf1N1B4_4732 at 5230.696 kb on + strand, within Pf1N1B4_4732 at 5230.756 kb on + strand, within Pf1N1B4_4732 at 5230.947 kb on + strand at 5231.008 kb on - strand at 5231.038 kb on + strand at 5231.038 kb on + strand at 5231.038 kb on + strand at 5231.039 kb on - strand at 5231.039 kb on - strand at 5231.039 kb on - strand at 5231.078 kb on + strand at 5231.078 kb on + strand at 5231.127 kb on + strand at 5231.128 kb on - strand at 5231.148 kb on + strand at 5231.148 kb on + strand at 5231.148 kb on + strand at 5231.371 kb on - strand, within Pf1N1B4_4733 at 5231.504 kb on + strand at 5231.514 kb on + strand, within Pf1N1B4_4733 at 5231.643 kb on - strand, within Pf1N1B4_4733 at 5231.643 kb on - strand, within Pf1N1B4_4733 at 5231.643 kb on - strand, within Pf1N1B4_4733 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5232.145 kb on + strand at 5232.145 kb on + strand, within Pf1N1B4_4734 at 5232.146 kb on - strand, within Pf1N1B4_4734 at 5232.213 kb on + strand, within Pf1N1B4_4734 at 5232.214 kb on - strand, within Pf1N1B4_4734 at 5232.397 kb on + strand, within Pf1N1B4_4734 at 5232.411 kb on + strand at 5232.476 kb on + strand, within Pf1N1B4_4734 at 5232.487 kb on - strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.572 kb on - strand at 5232.572 kb on - strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.831 kb on + strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.867 kb on + strand at 5232.868 kb on - strand, within Pf1N1B4_4735
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,230,177 + +0.7 5,230,177 + -1.3 5,230,177 + Pf1N1B4_4732 0.30 -0.4 5,230,183 + Pf1N1B4_4732 0.31 +0.4 5,230,184 - -0.0 5,230,184 - Pf1N1B4_4732 0.31 +0.5 5,230,411 + Pf1N1B4_4732 0.50 -0.2 5,230,507 + Pf1N1B4_4732 0.58 +0.2 5,230,507 + -2.3 5,230,687 + Pf1N1B4_4732 0.74 -1.0 5,230,688 - Pf1N1B4_4732 0.74 -1.1 5,230,696 + Pf1N1B4_4732 0.74 -0.2 5,230,696 + Pf1N1B4_4732 0.74 +0.3 5,230,756 + Pf1N1B4_4732 0.80 -0.9 5,230,947 + -0.0 5,231,008 - +0.2 5,231,038 + +2.0 5,231,038 + -0.6 5,231,038 + -0.1 5,231,039 - +0.1 5,231,039 - +1.3 5,231,039 - -0.2 5,231,078 + +1.7 5,231,078 + -0.5 5,231,127 + -0.7 5,231,128 - -0.7 5,231,148 + -0.6 5,231,148 + +0.5 5,231,148 + +1.8 5,231,371 - Pf1N1B4_4733 0.30 +2.0 5,231,504 + -0.0 5,231,514 + Pf1N1B4_4733 0.49 -0.5 5,231,643 - Pf1N1B4_4733 0.67 -1.4 5,231,643 - Pf1N1B4_4733 0.67 +0.6 5,231,643 - Pf1N1B4_4733 0.67 -1.7 5,231,698 + Pf1N1B4_4733 0.74 -1.8 5,231,698 + Pf1N1B4_4733 0.74 -1.3 5,232,145 + -0.0 5,232,145 + Pf1N1B4_4734 0.33 -1.0 5,232,146 - Pf1N1B4_4734 0.33 -0.9 5,232,213 + Pf1N1B4_4734 0.43 -0.0 5,232,214 - Pf1N1B4_4734 0.43 +2.0 5,232,397 + Pf1N1B4_4734 0.70 +0.6 5,232,411 + -1.0 5,232,476 + Pf1N1B4_4734 0.81 -0.4 5,232,487 - Pf1N1B4_4734 0.83 -0.3 5,232,505 + Pf1N1B4_4734 0.86 -1.6 5,232,505 + Pf1N1B4_4734 0.86 -2.3 5,232,572 - -0.7 5,232,572 - +1.0 5,232,831 + Pf1N1B4_4735 0.13 +1.0 5,232,831 + +1.6 5,232,831 + Pf1N1B4_4735 0.13 -2.3 5,232,832 - Pf1N1B4_4735 0.13 -0.4 5,232,832 - Pf1N1B4_4735 0.13 +0.7 5,232,832 - Pf1N1B4_4735 0.13 -0.2 5,232,867 + -1.0 5,232,868 - Pf1N1B4_4735 0.16 -1.1
Or see this region's nucleotide sequence