Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4667 and Pf1N1B4_4668 overlap by 4 nucleotides Pf1N1B4_4668 and Pf1N1B4_4669 overlap by 10 nucleotides
Pf1N1B4_4667 - Acriflavin resistance plasma membrane protein, at 5,146,584 to 5,148,125
_4667
Pf1N1B4_4668 - Heavy metal RND efflux outer membrane protein, CzcC family, at 5,148,122 to 5,148,688
_4668
Pf1N1B4_4669 - Heavy metal RND efflux outer membrane protein, CzcC family, at 5,148,679 to 5,149,602
_4669
Position (kb) 5148
5149 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5147.248 kb on - strand, within Pf1N1B4_4667 at 5147.619 kb on + strand at 5147.620 kb on - strand, within Pf1N1B4_4667 at 5147.620 kb on - strand, within Pf1N1B4_4667 at 5147.715 kb on + strand, within Pf1N1B4_4667 at 5147.716 kb on - strand, within Pf1N1B4_4667 at 5147.716 kb on - strand, within Pf1N1B4_4667 at 5148.025 kb on - strand at 5148.239 kb on + strand, within Pf1N1B4_4668 at 5148.272 kb on + strand, within Pf1N1B4_4668 at 5148.273 kb on - strand, within Pf1N1B4_4668 at 5148.273 kb on - strand, within Pf1N1B4_4668 at 5148.273 kb on - strand, within Pf1N1B4_4668 at 5148.345 kb on - strand, within Pf1N1B4_4668 at 5148.570 kb on - strand, within Pf1N1B4_4668 at 5148.715 kb on + strand at 5148.716 kb on - strand at 5148.716 kb on - strand at 5148.929 kb on + strand, within Pf1N1B4_4669 at 5149.105 kb on + strand, within Pf1N1B4_4669 at 5149.143 kb on + strand, within Pf1N1B4_4669 at 5149.287 kb on + strand at 5149.334 kb on - strand, within Pf1N1B4_4669 at 5149.334 kb on - strand, within Pf1N1B4_4669
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,147,248 - Pf1N1B4_4667 0.43 +0.6 5,147,619 + +4.2 5,147,620 - Pf1N1B4_4667 0.67 +1.6 5,147,620 - Pf1N1B4_4667 0.67 -2.0 5,147,715 + Pf1N1B4_4667 0.73 -0.5 5,147,716 - Pf1N1B4_4667 0.73 -1.3 5,147,716 - Pf1N1B4_4667 0.73 -1.2 5,148,025 - -0.4 5,148,239 + Pf1N1B4_4668 0.21 -0.5 5,148,272 + Pf1N1B4_4668 0.26 -0.9 5,148,273 - Pf1N1B4_4668 0.27 -1.2 5,148,273 - Pf1N1B4_4668 0.27 -2.5 5,148,273 - Pf1N1B4_4668 0.27 -1.0 5,148,345 - Pf1N1B4_4668 0.39 -1.5 5,148,570 - Pf1N1B4_4668 0.79 -0.6 5,148,715 + -1.8 5,148,716 - -1.0 5,148,716 - -0.1 5,148,929 + Pf1N1B4_4669 0.27 -0.8 5,149,105 + Pf1N1B4_4669 0.46 -1.2 5,149,143 + Pf1N1B4_4669 0.50 -0.7 5,149,287 + -0.4 5,149,334 - Pf1N1B4_4669 0.71 +0.0 5,149,334 - Pf1N1B4_4669 0.71 -2.0
Or see this region's nucleotide sequence