Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4595 and Pf1N1B4_4596 are separated by 99 nucleotides Pf1N1B4_4596 and Pf1N1B4_4597 are separated by 65 nucleotides Pf1N1B4_4597 and Pf1N1B4_4598 are separated by 92 nucleotides
Pf1N1B4_4595 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,067,954 to 5,069,408
_4595
Pf1N1B4_4596 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 5,069,508 to 5,070,275
_4596
Pf1N1B4_4597 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 5,070,341 to 5,071,099
_4597
Pf1N1B4_4598 - Ribonuclease HI (EC 3.1.26.4), at 5,071,192 to 5,071,644
_4598
Position (kb)
5069
5070
5071 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5068.682 kb on - strand, within Pf1N1B4_4595 at 5068.891 kb on + strand, within Pf1N1B4_4595 at 5069.516 kb on + strand at 5069.785 kb on + strand, within Pf1N1B4_4596 at 5069.785 kb on + strand at 5069.917 kb on + strand, within Pf1N1B4_4596 at 5069.917 kb on + strand at 5069.917 kb on + strand, within Pf1N1B4_4596 at 5070.046 kb on + strand, within Pf1N1B4_4596 at 5070.046 kb on + strand, within Pf1N1B4_4596 at 5070.047 kb on - strand at 5070.047 kb on - strand at 5070.222 kb on + strand at 5070.222 kb on + strand at 5070.223 kb on - strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.236 kb on - strand at 5070.270 kb on + strand at 5070.974 kb on + strand, within Pf1N1B4_4597 at 5070.978 kb on + strand at 5071.003 kb on - strand at 5071.097 kb on + strand at 5071.100 kb on + strand at 5071.101 kb on - strand at 5071.109 kb on + strand at 5071.205 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 5,068,682 - Pf1N1B4_4595 0.50 +1.1 5,068,891 + Pf1N1B4_4595 0.64 +0.1 5,069,516 + +0.3 5,069,785 + Pf1N1B4_4596 0.36 -0.8 5,069,785 + -1.9 5,069,917 + Pf1N1B4_4596 0.53 -0.7 5,069,917 + +1.1 5,069,917 + Pf1N1B4_4596 0.53 +0.8 5,070,046 + Pf1N1B4_4596 0.70 -2.3 5,070,046 + Pf1N1B4_4596 0.70 -1.7 5,070,047 - -0.9 5,070,047 - +0.1 5,070,222 + +0.7 5,070,222 + -0.6 5,070,223 - +0.4 5,070,235 + -0.9 5,070,235 + -1.1 5,070,235 + +0.1 5,070,235 + +2.1 5,070,235 + +1.1 5,070,235 + +1.1 5,070,235 + +0.6 5,070,236 - +1.1 5,070,270 + -0.7 5,070,974 + Pf1N1B4_4597 0.83 +0.1 5,070,978 + -1.5 5,071,003 - -1.5 5,071,097 + -2.9 5,071,100 + +0.6 5,071,101 - -0.9 5,071,109 + +0.6 5,071,205 + +0.6
Or see this region's nucleotide sequence