Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4349 and Pf1N1B4_4350 are separated by 89 nucleotides Pf1N1B4_4350 and Pf1N1B4_4351 overlap by 4 nucleotides Pf1N1B4_4351 and Pf1N1B4_4352 overlap by 4 nucleotides
Pf1N1B4_4349 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 4,805,007 to 4,806,104
_4349
Pf1N1B4_4350 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 4,806,194 to 4,807,078
_4350
Pf1N1B4_4351 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 4,807,075 to 4,807,989
_4351
Pf1N1B4_4352 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 4,807,986 to 4,809,128
_4352
Position (kb)
4806
4807
4808 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4805.242 kb on + strand at 4805.390 kb on - strand, within Pf1N1B4_4349 at 4805.434 kb on + strand, within Pf1N1B4_4349 at 4805.435 kb on - strand, within Pf1N1B4_4349 at 4805.435 kb on - strand, within Pf1N1B4_4349 at 4805.453 kb on - strand, within Pf1N1B4_4349 at 4805.720 kb on - strand, within Pf1N1B4_4349 at 4805.720 kb on - strand, within Pf1N1B4_4349 at 4805.824 kb on + strand at 4805.825 kb on - strand, within Pf1N1B4_4349 at 4805.924 kb on + strand, within Pf1N1B4_4349 at 4805.942 kb on - strand at 4805.998 kb on + strand at 4806.066 kb on + strand at 4806.067 kb on - strand at 4806.127 kb on + strand at 4806.128 kb on - strand at 4806.139 kb on + strand at 4806.139 kb on + strand at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.797 kb on - strand, within Pf1N1B4_4350 at 4806.874 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4807.024 kb on - strand at 4807.024 kb on - strand at 4807.028 kb on - strand at 4807.445 kb on + strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.664 kb on + strand, within Pf1N1B4_4351 at 4807.665 kb on - strand, within Pf1N1B4_4351 at 4807.665 kb on - strand at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.773 kb on - strand, within Pf1N1B4_4351 at 4807.820 kb on + strand, within Pf1N1B4_4351 at 4807.832 kb on + strand, within Pf1N1B4_4351 at 4807.841 kb on - strand at 4808.029 kb on + strand at 4808.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 4,805,242 + -0.8 4,805,390 - Pf1N1B4_4349 0.35 +0.5 4,805,434 + Pf1N1B4_4349 0.39 -0.3 4,805,435 - Pf1N1B4_4349 0.39 +0.2 4,805,435 - Pf1N1B4_4349 0.39 +0.3 4,805,453 - Pf1N1B4_4349 0.41 -0.2 4,805,720 - Pf1N1B4_4349 0.65 +0.5 4,805,720 - Pf1N1B4_4349 0.65 -1.4 4,805,824 + -1.4 4,805,825 - Pf1N1B4_4349 0.74 -0.4 4,805,924 + Pf1N1B4_4349 0.84 +0.2 4,805,942 - -0.8 4,805,998 + +0.2 4,806,066 + -0.5 4,806,067 - -0.8 4,806,127 + -1.4 4,806,128 - -2.1 4,806,139 + -0.1 4,806,139 + -0.8 4,806,633 + Pf1N1B4_4350 0.50 +0.2 4,806,633 + Pf1N1B4_4350 0.50 -0.6 4,806,633 + Pf1N1B4_4350 0.50 -1.8 4,806,633 + Pf1N1B4_4350 0.50 -0.4 4,806,633 + Pf1N1B4_4350 0.50 -0.9 4,806,634 - Pf1N1B4_4350 0.50 -0.2 4,806,634 - Pf1N1B4_4350 0.50 +0.5 4,806,634 - Pf1N1B4_4350 0.50 +0.2 4,806,634 - Pf1N1B4_4350 0.50 -0.7 4,806,634 - Pf1N1B4_4350 0.50 +0.5 4,806,797 - Pf1N1B4_4350 0.68 -1.7 4,806,874 - Pf1N1B4_4350 0.77 -0.4 4,806,940 - Pf1N1B4_4350 0.84 -2.6 4,806,940 - Pf1N1B4_4350 0.84 +1.4 4,807,024 - -1.2 4,807,024 - -0.8 4,807,028 - +2.0 4,807,445 + Pf1N1B4_4351 0.40 +1.6 4,807,446 - Pf1N1B4_4351 0.41 +0.8 4,807,446 - Pf1N1B4_4351 0.41 +0.7 4,807,452 - Pf1N1B4_4351 0.41 -0.3 4,807,452 - Pf1N1B4_4351 0.41 +0.9 4,807,467 - Pf1N1B4_4351 0.43 -0.0 4,807,467 - Pf1N1B4_4351 0.43 -1.6 4,807,467 - Pf1N1B4_4351 0.43 +0.5 4,807,664 + Pf1N1B4_4351 0.64 +0.1 4,807,665 - Pf1N1B4_4351 0.64 -1.4 4,807,665 - +0.2 4,807,745 + Pf1N1B4_4351 0.73 -1.8 4,807,745 + Pf1N1B4_4351 0.73 +1.2 4,807,746 - Pf1N1B4_4351 0.73 -1.8 4,807,746 - Pf1N1B4_4351 0.73 -2.4 4,807,746 - Pf1N1B4_4351 0.73 +3.1 4,807,746 - Pf1N1B4_4351 0.73 +1.3 4,807,773 - Pf1N1B4_4351 0.76 +0.0 4,807,820 + Pf1N1B4_4351 0.81 -0.0 4,807,832 + Pf1N1B4_4351 0.83 +0.4 4,807,841 - -0.4 4,808,029 + +0.9 4,808,030 - +1.2
Or see this region's nucleotide sequence