Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4229 and Pf1N1B4_4230 are separated by 148 nucleotides
Pf1N1B4_4229 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 4,684,339 to 4,687,173
_4229
Pf1N1B4_4230 - Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7), at 4,687,322 to 4,689,583
_4230
Position (kb)
4687
4688
4689
4690 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4686.596 kb on - strand, within Pf1N1B4_4229 at 4686.702 kb on - strand, within Pf1N1B4_4229 at 4686.752 kb on - strand, within Pf1N1B4_4229 at 4686.900 kb on + strand at 4686.900 kb on + strand at 4686.901 kb on - strand at 4687.127 kb on + strand at 4687.127 kb on + strand at 4687.128 kb on - strand at 4687.128 kb on - strand at 4687.171 kb on + strand at 4687.171 kb on + strand at 4687.172 kb on - strand at 4687.176 kb on + strand at 4687.176 kb on + strand at 4687.177 kb on - strand at 4687.312 kb on - strand at 4687.312 kb on - strand at 4687.413 kb on + strand at 4687.413 kb on + strand at 4687.413 kb on + strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.484 kb on - strand at 4687.484 kb on - strand at 4687.758 kb on + strand, within Pf1N1B4_4230 at 4687.763 kb on - strand, within Pf1N1B4_4230 at 4687.763 kb on - strand, within Pf1N1B4_4230 at 4687.792 kb on - strand, within Pf1N1B4_4230 at 4687.988 kb on + strand, within Pf1N1B4_4230 at 4687.988 kb on + strand, within Pf1N1B4_4230 at 4688.544 kb on + strand at 4688.544 kb on + strand, within Pf1N1B4_4230 at 4688.853 kb on + strand, within Pf1N1B4_4230 at 4689.036 kb on + strand, within Pf1N1B4_4230 at 4689.121 kb on + strand, within Pf1N1B4_4230 at 4689.165 kb on + strand, within Pf1N1B4_4230 at 4689.291 kb on + strand, within Pf1N1B4_4230 at 4689.323 kb on - strand, within Pf1N1B4_4230 at 4689.439 kb on - strand at 4689.439 kb on - strand at 4689.445 kb on - strand at 4689.738 kb on - strand at 4689.739 kb on + strand at 4689.740 kb on - strand at 4689.764 kb on - strand at 4689.789 kb on + strand at 4689.842 kb on + strand at 4689.857 kb on + strand at 4689.858 kb on - strand at 4689.858 kb on - strand at 4689.860 kb on + strand at 4689.860 kb on + strand at 4689.861 kb on - strand at 4689.861 kb on - strand at 4689.870 kb on - strand at 4689.870 kb on - strand at 4689.883 kb on - strand at 4689.959 kb on - strand at 4690.076 kb on + strand at 4690.076 kb on + strand at 4690.076 kb on + strand at 4690.077 kb on - strand at 4690.077 kb on - strand at 4690.077 kb on - strand at 4690.137 kb on + strand at 4690.138 kb on - strand at 4690.138 kb on - strand at 4690.138 kb on - strand at 4690.196 kb on + strand at 4690.196 kb on + strand at 4690.196 kb on + strand at 4690.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 4,686,596 - Pf1N1B4_4229 0.80 +0.8 4,686,702 - Pf1N1B4_4229 0.83 -0.0 4,686,752 - Pf1N1B4_4229 0.85 -1.0 4,686,900 + +0.8 4,686,900 + -0.1 4,686,901 - -0.4 4,687,127 + +0.2 4,687,127 + -0.5 4,687,128 - +0.6 4,687,128 - -1.2 4,687,171 + +0.8 4,687,171 + +0.8 4,687,172 - -2.8 4,687,176 + -1.4 4,687,176 + +1.6 4,687,177 - -1.4 4,687,312 - -0.0 4,687,312 - -0.2 4,687,413 + -0.8 4,687,413 + +2.0 4,687,413 + +0.8 4,687,414 - +2.5 4,687,414 - +0.7 4,687,414 - +1.5 4,687,414 - +0.3 4,687,414 - -0.5 4,687,414 - -0.1 4,687,414 - -1.3 4,687,484 - -1.4 4,687,484 - -1.6 4,687,758 + Pf1N1B4_4230 0.19 -1.1 4,687,763 - Pf1N1B4_4230 0.19 +3.0 4,687,763 - Pf1N1B4_4230 0.19 -0.1 4,687,792 - Pf1N1B4_4230 0.21 +0.7 4,687,988 + Pf1N1B4_4230 0.29 +0.2 4,687,988 + Pf1N1B4_4230 0.29 +0.5 4,688,544 + +0.2 4,688,544 + Pf1N1B4_4230 0.54 +0.4 4,688,853 + Pf1N1B4_4230 0.68 +1.2 4,689,036 + Pf1N1B4_4230 0.76 -0.0 4,689,121 + Pf1N1B4_4230 0.80 -0.2 4,689,165 + Pf1N1B4_4230 0.81 +0.8 4,689,291 + Pf1N1B4_4230 0.87 +0.3 4,689,323 - Pf1N1B4_4230 0.88 -0.5 4,689,439 - +1.6 4,689,439 - +0.2 4,689,445 - -0.4 4,689,738 - -0.2 4,689,739 + +0.9 4,689,740 - +0.9 4,689,764 - -0.0 4,689,789 + -1.0 4,689,842 + -0.0 4,689,857 + +0.2 4,689,858 - -2.6 4,689,858 - +0.2 4,689,860 + -1.0 4,689,860 + -0.1 4,689,861 - -0.4 4,689,861 - +0.6 4,689,870 - -3.6 4,689,870 - -0.8 4,689,883 - -0.6 4,689,959 - -0.0 4,690,076 + -0.2 4,690,076 + +0.2 4,690,076 + -2.1 4,690,077 - -0.8 4,690,077 - +2.5 4,690,077 - -2.1 4,690,137 + -2.2 4,690,138 - -0.2 4,690,138 - +1.6 4,690,138 - +0.1 4,690,196 + +0.4 4,690,196 + -0.1 4,690,196 + -2.4 4,690,357 + +0.2
Or see this region's nucleotide sequence