Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_415 and Pf1N1B4_416 are separated by 57 nucleotides Pf1N1B4_416 and Pf1N1B4_417 are separated by 16 nucleotides Pf1N1B4_417 and Pf1N1B4_418 are separated by 216 nucleotides
Pf1N1B4_415 - Lipase precursor (EC 3.1.1.3), at 484,593 to 485,471
_415
Pf1N1B4_416 - FIG003620: Proteophosphoglycan precursor (Fragment), at 485,529 to 486,089
_416
Pf1N1B4_417 - lipoprotein, putative, at 486,106 to 486,711
_417
Pf1N1B4_418 - Molybdenum cofactor biosynthesis protein MoaA, at 486,928 to 487,896
_418
Position (kb)
485
486
487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 484.588 kb on + strand at 484.588 kb on + strand at 484.589 kb on - strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.593 kb on - strand at 484.617 kb on - strand at 484.624 kb on - strand at 484.624 kb on - strand at 484.680 kb on + strand at 484.870 kb on - strand, within Pf1N1B4_415 at 484.874 kb on - strand at 484.996 kb on + strand, within Pf1N1B4_415 at 485.124 kb on + strand, within Pf1N1B4_415 at 485.124 kb on + strand, within Pf1N1B4_415 at 485.125 kb on - strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand at 485.185 kb on - strand, within Pf1N1B4_415 at 485.185 kb on - strand, within Pf1N1B4_415 at 485.185 kb on - strand, within Pf1N1B4_415 at 485.187 kb on + strand at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.191 kb on - strand at 485.209 kb on + strand, within Pf1N1B4_415 at 485.258 kb on + strand, within Pf1N1B4_415 at 485.259 kb on - strand, within Pf1N1B4_415 at 485.259 kb on - strand, within Pf1N1B4_415 at 485.280 kb on + strand, within Pf1N1B4_415 at 485.280 kb on + strand, within Pf1N1B4_415 at 485.280 kb on + strand at 485.319 kb on + strand, within Pf1N1B4_415 at 485.430 kb on + strand at 485.431 kb on - strand at 485.460 kb on - strand at 485.460 kb on - strand at 485.469 kb on + strand at 485.470 kb on - strand at 485.630 kb on - strand, within Pf1N1B4_416 at 485.630 kb on - strand, within Pf1N1B4_416 at 485.630 kb on - strand, within Pf1N1B4_416 at 485.630 kb on - strand, within Pf1N1B4_416 at 485.680 kb on + strand, within Pf1N1B4_416 at 485.681 kb on - strand, within Pf1N1B4_416 at 485.724 kb on - strand, within Pf1N1B4_416 at 485.724 kb on - strand, within Pf1N1B4_416 at 485.878 kb on + strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand at 485.953 kb on + strand, within Pf1N1B4_416 at 485.953 kb on + strand, within Pf1N1B4_416 at 485.954 kb on - strand, within Pf1N1B4_416 at 485.954 kb on - strand, within Pf1N1B4_416 at 485.954 kb on - strand, within Pf1N1B4_416 at 485.956 kb on + strand at 485.956 kb on + strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 486.107 kb on + strand at 486.108 kb on - strand at 486.141 kb on - strand at 486.196 kb on + strand, within Pf1N1B4_417 at 486.216 kb on + strand, within Pf1N1B4_417 at 486.216 kb on + strand, within Pf1N1B4_417 at 486.216 kb on + strand, within Pf1N1B4_417 at 486.217 kb on - strand, within Pf1N1B4_417 at 486.226 kb on - strand, within Pf1N1B4_417 at 486.236 kb on + strand, within Pf1N1B4_417 at 486.365 kb on + strand, within Pf1N1B4_417 at 486.365 kb on + strand, within Pf1N1B4_417 at 486.365 kb on + strand at 486.365 kb on + strand, within Pf1N1B4_417 at 486.374 kb on + strand, within Pf1N1B4_417 at 486.455 kb on + strand, within Pf1N1B4_417 at 486.455 kb on + strand, within Pf1N1B4_417 at 486.456 kb on - strand, within Pf1N1B4_417 at 486.495 kb on + strand, within Pf1N1B4_417 at 486.495 kb on + strand, within Pf1N1B4_417 at 486.496 kb on - strand, within Pf1N1B4_417 at 486.496 kb on - strand, within Pf1N1B4_417 at 486.545 kb on + strand, within Pf1N1B4_417 at 486.545 kb on + strand, within Pf1N1B4_417 at 486.545 kb on + strand, within Pf1N1B4_417 at 486.545 kb on + strand, within Pf1N1B4_417 at 486.549 kb on + strand, within Pf1N1B4_417 at 486.550 kb on - strand, within Pf1N1B4_417 at 486.576 kb on + strand, within Pf1N1B4_417 at 486.714 kb on + strand at 486.715 kb on - strand at 486.715 kb on - strand at 486.783 kb on + strand at 486.784 kb on - strand at 486.784 kb on - strand at 486.784 kb on - strand at 486.940 kb on - strand at 486.940 kb on - strand at 487.045 kb on - strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 484,588 + -0.3 484,588 + +0.3 484,589 - +1.4 484,592 + +1.5 484,592 + -0.2 484,592 + +0.9 484,592 + +0.4 484,592 + -0.3 484,593 - +0.5 484,617 - +0.3 484,624 - -1.0 484,624 - -0.5 484,680 + -0.5 484,870 - Pf1N1B4_415 0.32 -0.4 484,874 - +0.5 484,996 + Pf1N1B4_415 0.46 -1.0 485,124 + Pf1N1B4_415 0.60 -3.0 485,124 + Pf1N1B4_415 0.60 +0.9 485,125 - Pf1N1B4_415 0.61 -0.2 485,184 + Pf1N1B4_415 0.67 -0.7 485,184 + +2.3 485,184 + Pf1N1B4_415 0.67 +0.9 485,184 + Pf1N1B4_415 0.67 -1.3 485,184 + Pf1N1B4_415 0.67 -0.6 485,184 + Pf1N1B4_415 0.67 +1.0 485,184 + Pf1N1B4_415 0.67 +0.3 485,184 + -1.5 485,185 - Pf1N1B4_415 0.67 -1.5 485,185 - Pf1N1B4_415 0.67 -0.5 485,185 - Pf1N1B4_415 0.67 +0.8 485,187 + -1.8 485,187 + Pf1N1B4_415 0.68 -1.8 485,187 + Pf1N1B4_415 0.68 +0.5 485,187 + Pf1N1B4_415 0.68 +1.0 485,187 + Pf1N1B4_415 0.68 +1.8 485,188 - Pf1N1B4_415 0.68 -1.0 485,188 - Pf1N1B4_415 0.68 -0.2 485,188 - Pf1N1B4_415 0.68 -2.5 485,188 - Pf1N1B4_415 0.68 +1.5 485,188 - Pf1N1B4_415 0.68 -1.0 485,188 - Pf1N1B4_415 0.68 -0.6 485,190 + Pf1N1B4_415 0.68 -1.8 485,190 + Pf1N1B4_415 0.68 -0.7 485,190 + Pf1N1B4_415 0.68 -1.8 485,190 + Pf1N1B4_415 0.68 -0.2 485,191 - +1.1 485,209 + Pf1N1B4_415 0.70 -0.8 485,258 + Pf1N1B4_415 0.76 +1.3 485,259 - Pf1N1B4_415 0.76 +0.5 485,259 - Pf1N1B4_415 0.76 +0.8 485,280 + Pf1N1B4_415 0.78 +0.3 485,280 + Pf1N1B4_415 0.78 +1.2 485,280 + -1.3 485,319 + Pf1N1B4_415 0.83 -0.0 485,430 + -0.5 485,431 - -1.0 485,460 - +0.9 485,460 - +1.9 485,469 + -1.0 485,470 - -1.5 485,630 - Pf1N1B4_416 0.18 -1.9 485,630 - Pf1N1B4_416 0.18 -1.5 485,630 - Pf1N1B4_416 0.18 -0.7 485,630 - Pf1N1B4_416 0.18 -0.1 485,680 + Pf1N1B4_416 0.27 +0.5 485,681 - Pf1N1B4_416 0.27 -2.5 485,724 - Pf1N1B4_416 0.35 -2.3 485,724 - Pf1N1B4_416 0.35 -0.5 485,878 + Pf1N1B4_416 0.62 -3.4 485,879 - Pf1N1B4_416 0.62 -0.5 485,879 - Pf1N1B4_416 0.62 +1.3 485,879 - Pf1N1B4_416 0.62 -2.5 485,879 - Pf1N1B4_416 0.62 +0.5 485,879 - -0.5 485,953 + Pf1N1B4_416 0.76 -0.7 485,953 + Pf1N1B4_416 0.76 +0.6 485,954 - Pf1N1B4_416 0.76 -2.5 485,954 - Pf1N1B4_416 0.76 -1.8 485,954 - Pf1N1B4_416 0.76 -1.5 485,956 + +0.5 485,956 + Pf1N1B4_416 0.76 -0.5 485,957 - Pf1N1B4_416 0.76 -2.0 485,957 - Pf1N1B4_416 0.76 -1.0 485,957 - Pf1N1B4_416 0.76 -0.6 485,957 - Pf1N1B4_416 0.76 -2.0 486,107 + -0.5 486,108 - +0.5 486,141 - +0.0 486,196 + Pf1N1B4_417 0.15 +0.2 486,216 + Pf1N1B4_417 0.18 -1.8 486,216 + Pf1N1B4_417 0.18 -1.5 486,216 + Pf1N1B4_417 0.18 -0.0 486,217 - Pf1N1B4_417 0.18 -1.5 486,226 - Pf1N1B4_417 0.20 -0.7 486,236 + Pf1N1B4_417 0.21 +0.7 486,365 + Pf1N1B4_417 0.43 -1.0 486,365 + Pf1N1B4_417 0.43 -0.7 486,365 + -1.0 486,365 + Pf1N1B4_417 0.43 -0.2 486,374 + Pf1N1B4_417 0.44 +0.5 486,455 + Pf1N1B4_417 0.58 -1.3 486,455 + Pf1N1B4_417 0.58 +0.5 486,456 - Pf1N1B4_417 0.58 -0.9 486,495 + Pf1N1B4_417 0.64 +0.5 486,495 + Pf1N1B4_417 0.64 +0.8 486,496 - Pf1N1B4_417 0.64 -1.1 486,496 - Pf1N1B4_417 0.64 -0.5 486,545 + Pf1N1B4_417 0.72 -1.5 486,545 + Pf1N1B4_417 0.72 +0.5 486,545 + Pf1N1B4_417 0.72 +1.3 486,545 + Pf1N1B4_417 0.72 +1.3 486,549 + Pf1N1B4_417 0.73 -1.7 486,550 - Pf1N1B4_417 0.73 -1.0 486,576 + Pf1N1B4_417 0.78 -0.7 486,714 + -1.0 486,715 - +0.2 486,715 - +0.5 486,783 + +0.5 486,784 - -0.5 486,784 - -0.2 486,784 - +0.3 486,940 - +1.3 486,940 - -0.9 487,045 - Pf1N1B4_418 0.12 +0.2 487,064 + Pf1N1B4_418 0.14 -0.5 487,064 + Pf1N1B4_418 0.14 +1.3 487,064 + Pf1N1B4_418 0.14 -0.6 487,064 + Pf1N1B4_418 0.14 +2.5 487,065 - Pf1N1B4_418 0.14 -1.0 487,065 - Pf1N1B4_418 0.14 -0.7 487,065 - Pf1N1B4_418 0.14 -2.5 487,065 - Pf1N1B4_418 0.14 +1.3 487,065 - Pf1N1B4_418 0.14 +2.1 487,065 - Pf1N1B4_418 0.14 +0.5
Or see this region's nucleotide sequence