Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_411 and Pf1N1B4_412 are separated by 122 nucleotides Pf1N1B4_412 and Pf1N1B4_413 are separated by 270 nucleotides
Pf1N1B4_411 - ABC transporter for D-galactose/L-arabinose, substrate-binding component (from data) , at 480,413 to 481,417
_411
Pf1N1B4_412 - L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (from data) , at 481,540 to 482,358
_412
Pf1N1B4_413 - L-arabinolactonase (EC 3.1.1.15) (from data) , at 482,629 to 483,573
_413
Position (kb)
481
482
483 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 480.549 kb on + strand, within Pf1N1B4_411 at 480.549 kb on + strand, within Pf1N1B4_411 at 480.886 kb on + strand, within Pf1N1B4_411 at 480.906 kb on + strand, within Pf1N1B4_411 at 480.907 kb on - strand, within Pf1N1B4_411 at 480.907 kb on - strand, within Pf1N1B4_411 at 480.924 kb on + strand, within Pf1N1B4_411 at 480.924 kb on + strand, within Pf1N1B4_411 at 480.925 kb on - strand at 480.925 kb on - strand, within Pf1N1B4_411 at 480.925 kb on - strand, within Pf1N1B4_411 at 480.925 kb on - strand, within Pf1N1B4_411 at 480.925 kb on - strand, within Pf1N1B4_411 at 481.345 kb on + strand at 481.345 kb on + strand at 481.345 kb on + strand at 481.346 kb on - strand at 481.346 kb on - strand at 481.346 kb on - strand at 481.346 kb on - strand at 481.346 kb on - strand at 481.346 kb on - strand at 481.346 kb on - strand at 481.417 kb on + strand at 481.418 kb on - strand at 481.418 kb on - strand at 481.450 kb on + strand at 481.537 kb on + strand at 481.550 kb on + strand at 481.550 kb on + strand at 481.550 kb on + strand at 481.550 kb on + strand at 481.551 kb on - strand at 481.593 kb on + strand at 481.796 kb on + strand, within Pf1N1B4_412 at 481.796 kb on + strand, within Pf1N1B4_412 at 482.087 kb on - strand, within Pf1N1B4_412 at 482.123 kb on - strand, within Pf1N1B4_412 at 482.505 kb on - strand at 482.538 kb on - strand at 482.538 kb on - strand at 482.570 kb on + strand at 482.572 kb on + strand at 482.573 kb on - strand at 482.600 kb on + strand at 482.600 kb on + strand at 482.601 kb on - strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.669 kb on - strand at 482.669 kb on - strand at 482.732 kb on + strand, within Pf1N1B4_413 at 482.732 kb on + strand, within Pf1N1B4_413 at 482.732 kb on + strand, within Pf1N1B4_413 at 482.732 kb on + strand, within Pf1N1B4_413 at 482.733 kb on - strand, within Pf1N1B4_413 at 482.810 kb on + strand, within Pf1N1B4_413 at 482.811 kb on - strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand at 482.863 kb on + strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.930 kb on + strand, within Pf1N1B4_413 at 482.930 kb on + strand, within Pf1N1B4_413 at 482.930 kb on + strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 483.002 kb on + strand, within Pf1N1B4_413 at 483.003 kb on - strand, within Pf1N1B4_413 at 483.049 kb on + strand, within Pf1N1B4_413 at 483.049 kb on + strand, within Pf1N1B4_413 at 483.050 kb on - strand, within Pf1N1B4_413 at 483.058 kb on + strand, within Pf1N1B4_413 at 483.058 kb on + strand, within Pf1N1B4_413 at 483.059 kb on - strand, within Pf1N1B4_413 at 483.059 kb on - strand, within Pf1N1B4_413 at 483.059 kb on - strand, within Pf1N1B4_413 at 483.061 kb on + strand, within Pf1N1B4_413 at 483.124 kb on + strand, within Pf1N1B4_413
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 480,549 + Pf1N1B4_411 0.14 -1.5 480,549 + Pf1N1B4_411 0.14 -0.9 480,886 + Pf1N1B4_411 0.47 +0.5 480,906 + Pf1N1B4_411 0.49 +0.1 480,907 - Pf1N1B4_411 0.49 -3.2 480,907 - Pf1N1B4_411 0.49 +0.1 480,924 + Pf1N1B4_411 0.51 +0.2 480,924 + Pf1N1B4_411 0.51 -1.1 480,925 - +0.5 480,925 - Pf1N1B4_411 0.51 -0.8 480,925 - Pf1N1B4_411 0.51 -0.3 480,925 - Pf1N1B4_411 0.51 -0.1 480,925 - Pf1N1B4_411 0.51 -0.3 481,345 + +0.5 481,345 + +1.8 481,345 + -0.0 481,346 - -0.5 481,346 - +0.9 481,346 - -0.1 481,346 - -0.5 481,346 - +1.3 481,346 - +1.7 481,346 - +0.5 481,417 + +0.6 481,418 - -1.3 481,418 - -0.0 481,450 + -0.8 481,537 + -1.6 481,550 + -0.2 481,550 + -2.0 481,550 + +0.2 481,550 + -1.2 481,551 - +0.0 481,593 + -0.7 481,796 + Pf1N1B4_412 0.31 -0.7 481,796 + Pf1N1B4_412 0.31 -1.2 482,087 - Pf1N1B4_412 0.67 -1.2 482,123 - Pf1N1B4_412 0.71 +0.5 482,505 - +1.0 482,538 - +0.5 482,538 - +1.0 482,570 + +2.5 482,572 + +0.5 482,573 - -0.1 482,600 + -0.9 482,600 + +0.5 482,601 - +0.0 482,668 + +0.9 482,668 + -1.8 482,668 + -1.5 482,668 + +1.0 482,668 + -0.3 482,668 + +1.0 482,668 + -0.0 482,669 - +0.9 482,669 - -0.0 482,732 + Pf1N1B4_413 0.11 +1.2 482,732 + Pf1N1B4_413 0.11 -2.8 482,732 + Pf1N1B4_413 0.11 -1.1 482,732 + Pf1N1B4_413 0.11 +0.8 482,733 - Pf1N1B4_413 0.11 -0.5 482,810 + Pf1N1B4_413 0.19 -0.1 482,811 - Pf1N1B4_413 0.19 +0.2 482,863 + Pf1N1B4_413 0.25 -0.2 482,863 + Pf1N1B4_413 0.25 -1.1 482,863 + Pf1N1B4_413 0.25 -0.7 482,863 + Pf1N1B4_413 0.25 -0.6 482,863 + Pf1N1B4_413 0.25 -1.5 482,863 + Pf1N1B4_413 0.25 -0.1 482,863 + +1.5 482,863 + Pf1N1B4_413 0.25 -0.1 482,864 - Pf1N1B4_413 0.25 -2.5 482,864 - Pf1N1B4_413 0.25 +0.3 482,864 - Pf1N1B4_413 0.25 -0.4 482,864 - Pf1N1B4_413 0.25 +0.2 482,864 - Pf1N1B4_413 0.25 +0.8 482,864 - Pf1N1B4_413 0.25 -1.0 482,864 - Pf1N1B4_413 0.25 -0.7 482,864 - Pf1N1B4_413 0.25 +1.5 482,864 - Pf1N1B4_413 0.25 +0.2 482,867 + Pf1N1B4_413 0.25 -1.0 482,867 + Pf1N1B4_413 0.25 +0.2 482,867 + Pf1N1B4_413 0.25 -0.9 482,867 + Pf1N1B4_413 0.25 -3.1 482,868 - Pf1N1B4_413 0.25 -0.5 482,868 - Pf1N1B4_413 0.25 -0.5 482,868 - Pf1N1B4_413 0.25 +0.2 482,868 - Pf1N1B4_413 0.25 +1.5 482,868 - Pf1N1B4_413 0.25 +0.5 482,868 - Pf1N1B4_413 0.25 -0.1 482,868 - Pf1N1B4_413 0.25 -0.8 482,930 + Pf1N1B4_413 0.32 +0.5 482,930 + Pf1N1B4_413 0.32 -0.3 482,930 + Pf1N1B4_413 0.32 -2.5 482,931 - Pf1N1B4_413 0.32 -0.1 482,931 - Pf1N1B4_413 0.32 -2.2 482,931 - Pf1N1B4_413 0.32 -0.2 482,931 - Pf1N1B4_413 0.32 -1.1 482,931 - Pf1N1B4_413 0.32 -0.7 482,931 - Pf1N1B4_413 0.32 +0.2 483,002 + Pf1N1B4_413 0.39 -0.2 483,003 - Pf1N1B4_413 0.40 +1.4 483,049 + Pf1N1B4_413 0.44 +0.0 483,049 + Pf1N1B4_413 0.44 -2.5 483,050 - Pf1N1B4_413 0.45 -0.3 483,058 + Pf1N1B4_413 0.45 -0.3 483,058 + Pf1N1B4_413 0.45 +0.7 483,059 - Pf1N1B4_413 0.46 -0.8 483,059 - Pf1N1B4_413 0.46 +0.1 483,059 - Pf1N1B4_413 0.46 +0.9 483,061 + Pf1N1B4_413 0.46 +0.5 483,124 + Pf1N1B4_413 0.52 +0.5
Or see this region's nucleotide sequence