Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4104 and Pf1N1B4_4105 are separated by 47 nucleotides Pf1N1B4_4105 and Pf1N1B4_4106 are separated by 93 nucleotides Pf1N1B4_4106 and Pf1N1B4_4107 are separated by 240 nucleotides Pf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotides
Pf1N1B4_4104 - Probable transmembrane protein, at 4,552,366 to 4,552,851
_4104
Pf1N1B4_4105 - hypothetical protein, at 4,552,899 to 4,553,366
_4105
Pf1N1B4_4106 - Mesenchymal stem cell protein DSCD75, at 4,553,460 to 4,554,032
_4106
Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905
_4107
Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718
_4108
Position (kb)
4553
4554
4555 Strain fitness (log2 ratio)
-2
-1
0
1 at 4552.567 kb on + strand, within Pf1N1B4_4104 at 4552.567 kb on + strand, within Pf1N1B4_4104 at 4552.568 kb on - strand, within Pf1N1B4_4104 at 4552.568 kb on - strand, within Pf1N1B4_4104 at 4552.765 kb on - strand, within Pf1N1B4_4104 at 4553.212 kb on + strand, within Pf1N1B4_4105 at 4553.212 kb on + strand, within Pf1N1B4_4105 at 4553.213 kb on - strand at 4553.213 kb on - strand, within Pf1N1B4_4105 at 4553.386 kb on - strand at 4553.436 kb on + strand at 4553.440 kb on - strand at 4553.440 kb on - strand at 4553.446 kb on + strand at 4553.525 kb on + strand, within Pf1N1B4_4106 at 4553.549 kb on + strand, within Pf1N1B4_4106 at 4553.550 kb on - strand, within Pf1N1B4_4106 at 4553.550 kb on - strand at 4553.730 kb on + strand, within Pf1N1B4_4106 at 4553.731 kb on - strand at 4553.739 kb on - strand at 4553.871 kb on + strand, within Pf1N1B4_4106 at 4553.872 kb on - strand, within Pf1N1B4_4106 at 4553.872 kb on - strand, within Pf1N1B4_4106 at 4553.883 kb on - strand at 4553.890 kb on + strand, within Pf1N1B4_4106 at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.057 kb on - strand at 4554.057 kb on - strand at 4554.091 kb on - strand at 4554.420 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.903 kb on + strand at 4554.935 kb on + strand at 4554.935 kb on + strand at 4554.974 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 4,552,567 + Pf1N1B4_4104 0.41 +0.9 4,552,567 + Pf1N1B4_4104 0.41 -0.3 4,552,568 - Pf1N1B4_4104 0.42 +0.3 4,552,568 - Pf1N1B4_4104 0.42 +0.0 4,552,765 - Pf1N1B4_4104 0.82 -2.3 4,553,212 + Pf1N1B4_4105 0.67 +1.3 4,553,212 + Pf1N1B4_4105 0.67 +0.7 4,553,213 - +0.3 4,553,213 - Pf1N1B4_4105 0.67 -0.5 4,553,386 - -1.3 4,553,436 + -2.3 4,553,440 - +0.3 4,553,440 - -0.7 4,553,446 + +1.2 4,553,525 + Pf1N1B4_4106 0.11 -0.6 4,553,549 + Pf1N1B4_4106 0.16 -1.2 4,553,550 - Pf1N1B4_4106 0.16 +1.0 4,553,550 - -0.7 4,553,730 + Pf1N1B4_4106 0.47 -0.3 4,553,731 - +1.3 4,553,739 - -2.1 4,553,871 + Pf1N1B4_4106 0.72 -1.3 4,553,872 - Pf1N1B4_4106 0.72 -1.3 4,553,872 - Pf1N1B4_4106 0.72 -2.5 4,553,883 - -1.0 4,553,890 + Pf1N1B4_4106 0.75 -1.5 4,554,053 - +0.1 4,554,053 - -0.3 4,554,053 - -0.1 4,554,057 - +0.7 4,554,057 - -1.7 4,554,091 - +0.1 4,554,420 + Pf1N1B4_4107 0.23 -0.4 4,554,738 + Pf1N1B4_4107 0.73 -1.1 4,554,738 + Pf1N1B4_4107 0.73 +0.6 4,554,738 + Pf1N1B4_4107 0.73 +1.1 4,554,903 + -1.5 4,554,935 + -1.3 4,554,935 + -0.5 4,554,974 + -0.7 4,554,986 + +1.3 4,554,986 + +0.3 4,554,986 + -0.7
Or see this region's nucleotide sequence