Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4106

Experiment: Gentamicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4104 and Pf1N1B4_4105 are separated by 47 nucleotidesPf1N1B4_4105 and Pf1N1B4_4106 are separated by 93 nucleotidesPf1N1B4_4106 and Pf1N1B4_4107 are separated by 240 nucleotidesPf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotides Pf1N1B4_4104 - Probable transmembrane protein, at 4,552,366 to 4,552,851 _4104 Pf1N1B4_4105 - hypothetical protein, at 4,552,899 to 4,553,366 _4105 Pf1N1B4_4106 - Mesenchymal stem cell protein DSCD75, at 4,553,460 to 4,554,032 _4106 Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905 _4107 Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718 _4108 Position (kb) 4553 4554 4555Strain fitness (log2 ratio) -2 -1 0 1at 4552.567 kb on + strand, within Pf1N1B4_4104at 4552.567 kb on + strand, within Pf1N1B4_4104at 4552.568 kb on - strand, within Pf1N1B4_4104at 4552.568 kb on - strand, within Pf1N1B4_4104at 4552.765 kb on - strand, within Pf1N1B4_4104at 4553.212 kb on + strand, within Pf1N1B4_4105at 4553.212 kb on + strand, within Pf1N1B4_4105at 4553.213 kb on - strandat 4553.213 kb on - strand, within Pf1N1B4_4105at 4553.386 kb on - strandat 4553.436 kb on + strandat 4553.440 kb on - strandat 4553.440 kb on - strandat 4553.446 kb on + strandat 4553.525 kb on + strand, within Pf1N1B4_4106at 4553.549 kb on + strand, within Pf1N1B4_4106at 4553.550 kb on - strand, within Pf1N1B4_4106at 4553.550 kb on - strandat 4553.730 kb on + strand, within Pf1N1B4_4106at 4553.731 kb on - strandat 4553.739 kb on - strandat 4553.871 kb on + strand, within Pf1N1B4_4106at 4553.872 kb on - strand, within Pf1N1B4_4106at 4553.872 kb on - strand, within Pf1N1B4_4106at 4553.883 kb on - strandat 4553.890 kb on + strand, within Pf1N1B4_4106at 4554.053 kb on - strandat 4554.053 kb on - strandat 4554.053 kb on - strandat 4554.057 kb on - strandat 4554.057 kb on - strandat 4554.091 kb on - strandat 4554.420 kb on + strand, within Pf1N1B4_4107at 4554.738 kb on + strand, within Pf1N1B4_4107at 4554.738 kb on + strand, within Pf1N1B4_4107at 4554.738 kb on + strand, within Pf1N1B4_4107at 4554.903 kb on + strandat 4554.935 kb on + strandat 4554.935 kb on + strandat 4554.974 kb on + strandat 4554.986 kb on + strandat 4554.986 kb on + strandat 4554.986 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml
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4,552,567 + Pf1N1B4_4104 0.41 +0.9
4,552,567 + Pf1N1B4_4104 0.41 -0.3
4,552,568 - Pf1N1B4_4104 0.42 +0.3
4,552,568 - Pf1N1B4_4104 0.42 +0.0
4,552,765 - Pf1N1B4_4104 0.82 -2.3
4,553,212 + Pf1N1B4_4105 0.67 +1.3
4,553,212 + Pf1N1B4_4105 0.67 +0.7
4,553,213 - +0.3
4,553,213 - Pf1N1B4_4105 0.67 -0.5
4,553,386 - -1.3
4,553,436 + -2.3
4,553,440 - +0.3
4,553,440 - -0.7
4,553,446 + +1.2
4,553,525 + Pf1N1B4_4106 0.11 -0.6
4,553,549 + Pf1N1B4_4106 0.16 -1.2
4,553,550 - Pf1N1B4_4106 0.16 +1.0
4,553,550 - -0.7
4,553,730 + Pf1N1B4_4106 0.47 -0.3
4,553,731 - +1.3
4,553,739 - -2.1
4,553,871 + Pf1N1B4_4106 0.72 -1.3
4,553,872 - Pf1N1B4_4106 0.72 -1.3
4,553,872 - Pf1N1B4_4106 0.72 -2.5
4,553,883 - -1.0
4,553,890 + Pf1N1B4_4106 0.75 -1.5
4,554,053 - +0.1
4,554,053 - -0.3
4,554,053 - -0.1
4,554,057 - +0.7
4,554,057 - -1.7
4,554,091 - +0.1
4,554,420 + Pf1N1B4_4107 0.23 -0.4
4,554,738 + Pf1N1B4_4107 0.73 -1.1
4,554,738 + Pf1N1B4_4107 0.73 +0.6
4,554,738 + Pf1N1B4_4107 0.73 +1.1
4,554,903 + -1.5
4,554,935 + -1.3
4,554,935 + -0.5
4,554,974 + -0.7
4,554,986 + +1.3
4,554,986 + +0.3
4,554,986 + -0.7

Or see this region's nucleotide sequence