Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4091 and Pf1N1B4_4092 are separated by 196 nucleotides Pf1N1B4_4092 and Pf1N1B4_4093 are separated by 159 nucleotides
Pf1N1B4_4091 - Probable signal peptide protein, at 4,539,825 to 4,540,565
_4091
Pf1N1B4_4092 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 4,540,762 to 4,542,174
_4092
Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987
_4093
Position (kb)
4540
4541
4542
4543 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4539.808 kb on - strand at 4539.808 kb on - strand at 4539.824 kb on + strand at 4539.825 kb on - strand at 4539.825 kb on - strand at 4539.825 kb on - strand at 4539.832 kb on + strand at 4539.832 kb on + strand at 4539.862 kb on + strand at 4539.862 kb on + strand at 4539.862 kb on + strand at 4539.863 kb on - strand at 4539.863 kb on - strand at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.102 kb on - strand, within Pf1N1B4_4091 at 4540.102 kb on - strand, within Pf1N1B4_4091 at 4540.144 kb on - strand, within Pf1N1B4_4091 at 4540.152 kb on + strand, within Pf1N1B4_4091 at 4540.153 kb on - strand, within Pf1N1B4_4091 at 4540.285 kb on - strand, within Pf1N1B4_4091 at 4540.291 kb on - strand at 4540.291 kb on - strand, within Pf1N1B4_4091 at 4540.291 kb on - strand, within Pf1N1B4_4091 at 4540.291 kb on - strand, within Pf1N1B4_4091 at 4540.299 kb on + strand, within Pf1N1B4_4091 at 4540.404 kb on + strand at 4540.405 kb on - strand, within Pf1N1B4_4091 at 4540.405 kb on - strand, within Pf1N1B4_4091 at 4540.405 kb on - strand, within Pf1N1B4_4091 at 4540.464 kb on + strand, within Pf1N1B4_4091 at 4540.464 kb on + strand, within Pf1N1B4_4091 at 4540.563 kb on + strand at 4540.563 kb on + strand at 4540.563 kb on + strand at 4540.564 kb on - strand at 4540.590 kb on + strand at 4540.591 kb on - strand at 4540.760 kb on + strand at 4540.822 kb on + strand at 4540.822 kb on + strand at 4540.823 kb on - strand at 4541.071 kb on + strand at 4541.297 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.444 kb on - strand, within Pf1N1B4_4092 at 4541.630 kb on - strand, within Pf1N1B4_4092 at 4541.630 kb on - strand at 4541.833 kb on + strand at 4541.834 kb on - strand, within Pf1N1B4_4092 at 4541.894 kb on - strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.897 kb on - strand, within Pf1N1B4_4092 at 4541.897 kb on - strand, within Pf1N1B4_4092 at 4541.969 kb on + strand, within Pf1N1B4_4092 at 4541.969 kb on + strand, within Pf1N1B4_4092 at 4541.986 kb on + strand, within Pf1N1B4_4092 at 4541.986 kb on + strand, within Pf1N1B4_4092 at 4542.072 kb on + strand at 4542.112 kb on + strand at 4542.115 kb on + strand at 4542.240 kb on + strand at 4542.240 kb on + strand at 4542.243 kb on - strand at 4542.307 kb on + strand at 4542.320 kb on + strand at 4542.320 kb on + strand at 4542.484 kb on + strand, within Pf1N1B4_4093 at 4542.656 kb on - strand, within Pf1N1B4_4093 at 4542.712 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.929 kb on - strand at 4542.958 kb on + strand at 4542.959 kb on - strand at 4543.021 kb on + strand at 4543.167 kb on + strand at 4543.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 4,539,808 - +0.9 4,539,808 - +1.1 4,539,824 + +0.1 4,539,825 - +0.3 4,539,825 - -1.0 4,539,825 - +0.6 4,539,832 + -1.3 4,539,832 + -0.9 4,539,862 + +0.9 4,539,862 + +1.4 4,539,862 + -2.5 4,539,863 - +0.3 4,539,863 - -1.5 4,540,024 - Pf1N1B4_4091 0.27 -0.7 4,540,024 - Pf1N1B4_4091 0.27 +2.3 4,540,024 - Pf1N1B4_4091 0.27 -0.0 4,540,024 - Pf1N1B4_4091 0.27 +0.1 4,540,102 - Pf1N1B4_4091 0.37 +0.5 4,540,102 - Pf1N1B4_4091 0.37 +0.6 4,540,144 - Pf1N1B4_4091 0.43 +1.0 4,540,152 + Pf1N1B4_4091 0.44 +0.9 4,540,153 - Pf1N1B4_4091 0.44 -0.3 4,540,285 - Pf1N1B4_4091 0.62 -0.7 4,540,291 - -0.1 4,540,291 - Pf1N1B4_4091 0.63 +0.6 4,540,291 - Pf1N1B4_4091 0.63 -2.5 4,540,291 - Pf1N1B4_4091 0.63 -1.0 4,540,299 + Pf1N1B4_4091 0.64 +1.7 4,540,404 + -0.7 4,540,405 - Pf1N1B4_4091 0.78 +0.3 4,540,405 - Pf1N1B4_4091 0.78 -0.7 4,540,405 - Pf1N1B4_4091 0.78 -0.0 4,540,464 + Pf1N1B4_4091 0.86 +1.2 4,540,464 + Pf1N1B4_4091 0.86 -0.3 4,540,563 + +0.7 4,540,563 + +0.3 4,540,563 + -0.1 4,540,564 - +0.0 4,540,590 + +0.3 4,540,591 - -0.8 4,540,760 + +1.3 4,540,822 + +0.4 4,540,822 + -1.0 4,540,823 - +0.6 4,541,071 + -0.7 4,541,297 - Pf1N1B4_4092 0.38 +0.5 4,541,423 - Pf1N1B4_4092 0.47 -1.1 4,541,423 - Pf1N1B4_4092 0.47 -1.3 4,541,423 - Pf1N1B4_4092 0.47 -0.3 4,541,423 - +1.6 4,541,423 - Pf1N1B4_4092 0.47 +1.9 4,541,444 - Pf1N1B4_4092 0.48 -0.1 4,541,630 - Pf1N1B4_4092 0.61 -0.8 4,541,630 - +0.3 4,541,833 + -0.7 4,541,834 - Pf1N1B4_4092 0.76 +0.8 4,541,894 - Pf1N1B4_4092 0.80 -0.9 4,541,896 + Pf1N1B4_4092 0.80 +1.9 4,541,896 + Pf1N1B4_4092 0.80 +0.3 4,541,896 + Pf1N1B4_4092 0.80 +1.9 4,541,897 - Pf1N1B4_4092 0.80 +1.3 4,541,897 - Pf1N1B4_4092 0.80 +0.1 4,541,969 + Pf1N1B4_4092 0.85 -2.0 4,541,969 + Pf1N1B4_4092 0.85 -0.8 4,541,986 + Pf1N1B4_4092 0.87 -1.1 4,541,986 + Pf1N1B4_4092 0.87 -0.7 4,542,072 + -2.7 4,542,112 + -2.0 4,542,115 + +0.5 4,542,240 + +0.3 4,542,240 + +0.1 4,542,243 - -2.0 4,542,307 + +1.1 4,542,320 + -0.1 4,542,320 + -0.3 4,542,484 + Pf1N1B4_4093 0.23 +0.1 4,542,656 - Pf1N1B4_4093 0.49 +0.9 4,542,712 - Pf1N1B4_4093 0.58 +1.5 4,542,770 - Pf1N1B4_4093 0.67 -0.3 4,542,770 - Pf1N1B4_4093 0.67 -1.1 4,542,929 - +0.3 4,542,958 + +1.3 4,542,959 - +0.3 4,543,021 + -0.7 4,543,167 + +0.6 4,543,167 + +0.3
Or see this region's nucleotide sequence